CVM TRANSPORT MEDIA

Cat. no. R96 CVM Transport, 15x120mm Polypropylene Centrifuge
Tube, 2ml
20 tubes/box

INTENDED USE

Hardy Diagnostics CVM Transport Media is provided in plastic centrifuge tubes and is used for the collection and transport of clinical specimens for the recovery of chlamydial, viral, and mycoplasmal agents including: Herpes Simplex Type I, Herpes Simplex Type II, Cytomegalovirus (CMV), Influenzae A, Influenzae B, Respiratory Syncytial Virus (RSV), Echovirus, Adenovirus, Chlamydia trachomatis , Chlamydia pneumoniae , Mycoplasma hominis , Mycoplasma pneumoniae and Ureaplasma urealyticum .

SUMMARY

CVM Transport consists of Modified Hank's Balanced Salt Solution supplemented with bovine serum albumin, sucrose, glutamic acid, and gelatin. A buffer is used to maintain a pH of 7.3 +/- 0.2. Phenol red is used as a pH indicator. Amphotericin B (4ug/ml), colistin (7.5ug/ml), and vancomycin (100ug/ml) are added to inhibit contaminants. The media contains cryoprotectorants to ensure viability of organisms during freezing and thawing.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), hemolysis, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat and freezing at temperatures above -70ºC.

PRECAUTIONS

PROCEDURE

Aseptically collect sample. Use swabs and shafts that are non-toxic to chlamydial, mycoplasmal, ureaplasmal, and viral agents, such as dacron or nylon flocked swabs on plastic shafts. Do not use cotton or calcium alginate swabs for collection of specimens.

After collection of specimen, remove cap from tube, insert swab. Submerge the tip of the swab into the media so the tip contacts the bottom of the tube. Break off the top third of the swab by bending the shaft against the rim of the tube. The shaft is scored to facilitate breaking. Re-cap the tube securely. Label the sample. Transport immediately to the lab at 2-30ºC. within 48 hours. Upon arrival to the lab, refrigerate at 2-8ºC. For long-term storage, freeze at -70ºC. Do not freeze at temperatures higher than -70ºC. For more information, consult listed references. (2-5)

More than one tube may be submitted if more than one type of culture is requested to ensure adequate specimen for culture set-ups.

All specimens should be shipped in compliance with all federal, state and hospital guidelines.

INTERPRETATION OF RESULTS

Consult listed references for isolation and identification procedures for specific organisms recovered from transport media. (2-5)

LIMITATIONS

Inoculate specimens as soon after collection as possible. This transport has been validated to maintain viability of organisms during transport at room temperature for 48 hours. If there is to be a delay in inoculating, store at 2-8ºC. For long-term storage of specimens for recovery of chlamydial, viral, and mycoplasmal agents, freeze at -70ºC. Freezing at higher than -70ºC. is not recommended.

Repeated freezing and thawing of frozen specimens may reduce the recovery of viable organisms.

Nylon flocked swabs, dacron or rayon tipped swabs are recommended. Calcium alginate or cotton swabs, as well as wooden stick swabs, should not be used.

Determinations for recovery of specific serotypes of C. trachomatis were not included in the study (see Performance Characteristics section).

Data represented in the Performance Characteristics section is for culture only. Performance has not been established for EIA testing. It is recommended that each laboratory establish performance characteristics of this product in conjunction with its chlamydial, viral, and mycoplasmal testing methods.

If culturing for cytomegalovirus and transport is at room temperature, the transport time should not be greater than 24 hours. If transported at 2-8ºC. the transport time should not be greater than 72 hours.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, incinerators, incubators, centrifuge, pasteur pipets, culture media and cell culture media, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

After inoculation to the CVM Transport Media, the organisms listed below are held at 2-30ºC., for the indicated time, and then subcultured to appropriate culture media.

Test Organisms Time Reaction
Mycoplasma pneumoniae
ATCC ® 15531
48hr Viability maintained
Herpes Simplex Type I
McIntyre
ATCC ® VR-539

48hr Viability maintained

Toxicity: A 0.3ml aliquot of the CVM Transport is inoculated into MRC-5 shell vials. The vials are incubated for 18-24 hours at 35ºC. Cells are examined microscopically for the presence of cytopathic effect (CPE).

User Quality Control

Physical Appearance

CVM Transport Media should appear clear, and light peach in color.

CVM Transport Media

CVM Transport Media (Cat. no. R96).

PERFORMANCE CHARACTERISTICS

CVM Transport was compared to a commercially available multimicrobe transport media. Both transports are intended for use in the transport and maintenance of specimens for the recovery of viral, chlamydial, mycoplasmal and ureaplasmal agents. The results for the thirteen organisms (wild-type strains) tested in CVM Transports were as follows:

Organism
(Wild-Type Strains:
one isolate/organism)

Inoculum
Concentration
Maximum Holding Time
(Percent of recovery was not determined)
2-8 o C. 15-30 o C.
Herpes Simplex Type I 10 -3 TCID50 96 hours 96 hours
Herpes Simplex Type II 10 -3 TCID50 96 hours 96 hours
Cytomegalovirus n/t 72 hours 24 hours
Influenzae A 10 -1 TCID50 72 hours 72 hours
Influenzae B 10 -1 TCID50 72 hours 48 hours
RSV 10 -1 TCID50 72 hours 72 hours
Echovirus 10 -1 TCID50 96 hours 96 hours
Adenovirus 10 -1 TCID50 96 hours 72 hours
Chlamydia pneumoniae * 10 -1 IFU 72 hours 48 hours
Chlamydia trachomatis 10 -1 IFU 72 hours 48 hours
Mycoplasma hominis 10 -1 CFU 96 hours 96 hours
Mycoplasma pneumoniae 10 -1 CFU 96 hours 72 hours
Ureaplasma urealyticum 10 -1 CFU 96 hours 96 hours

* Chlamydia pneumoniae ATCC ® CCL-23.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Versalovic, J., et al. Manual of Clinical Microbiology. American Society for Microbiology, Washington, D.C.

3. Tille, P.M., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Clyde, W.A., et al. 1984. Cumitech 19; Laboratory Diagnosis of Chlamydial and Mycoplasmal Infections . Coordinating ed., W.L. Drew. American Society for Microbiology, Washington, D.C.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

6. Daley, P., Castriciano, S., Chernesky, M., and Smieja, M. 2006. Comparison of Flocked and Rayon Swabs for Collection of Respiratory Epithelial Cells from Uninfected Volunteers and Symptomatic Patients. Journal of Clinical Microbiology, p. 2265-2267.

7. Castriciano, S., Petrich, A., Smieja, M., and Chernesky, M.A. 2007. Flocked Swabs and UTM-RT are Pre-analytical Tools Suitable for Rapid Antigen Kits, Direct Immunofluorescence, Culture and PCR Diagnostics Assay for Viral Infections. 17th European Congress of Clinical Microbiology and Infectious Diseases Munich, Germany.


ATCC is a registered trademark of the American Type Culture Collection.

121415hh