CAMPYGEN™ COMPACT

Cat. no. CN020C CampyGen™ Compact 20 sachets/package
Cat. no. AN005C CampyGen™ Compact Sealing Clips 5 clips/box
Cat. no. AG020C Plastic Pouches 20 pouches/box

INTENDED USE

Oxoid CampyGen™ Compact for one or two petri dishes, is a simple system for generating microaerobic conditions, that is required for culturing microaerophilic organisms, such as Campylobacter spp.

SUMMARY

The system consists of a plastic pouch, sealing clip, and a paper gas generating sachet. The paper sachet contains ascorbic acid which reacts on contact with air to produce the microaerobic conditions for the growth of microaerophilic organisms. No catalyst is needed. The addition of water is not required. Potentially explosive hydrogen gas is not produced as with other older systems.

FORMULA

Ascorbic acid is the reactive component within each paper sachet.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-25ºC. Under these conditions, the CampyGen™ Compact sachets will retain their activity until the expiration date declared on the outer box and on the foil wrapped sachet. Products should not be used if there are any signs of deterioration (tears), or if the expiration date has passed. Protect from excessive heat and freezing.

PRECAUTIONS

PROCEDURE

Method of Use:

1. Place two inoculated media plates in the plastic pouch. Disposable plastic petri dishes should be of the vented variety to aid gas transfer between the interior and exterior of the plates. If only one plate is to be inoculated, an uninoculated plate should also be placed in the plastic pouch to prevent further activity as the volume of O 2 and CO 2 is critical.

2. Tear open a CampyGen™ Compact foil sachet at the tear-nick indicated. Remove the CampyGen™ Compact paper sachet from within. The sachet is activated upon exposure to air.

3. Immediately place the CampyGen™ Compact paper sachet in the plastic pouch with the plates.

Note: The CampyGen™ Compact paper sachet will become warm to the touch on exposure to air.

4. Expel excess air from the plastic pouch. Seal the plastic pouch immediately with the CampyGen™ Compact Sealing Clip (Cat. no. AN005C) or equivalent.

Note: The time taken between opening the foil sachet and sealing the plastic pouch should not exceed one minute. Extended exposure will result in loss of reactivity, and full anaerobic conditions may not be achieved in the pouch.

5. Incubate appropriately.

6. After the incubation period remove the plates or ID panel and examine for the presence of colonies or biochemical reaction. If the plates require reincubation then a fresh CampyGen™ Compact sachet must be used following steps 2-5 described above.

Note: The plates may be initially inspected through the transparent plastic pouch. If the bag is not opened, a fresh CampyGen™ Compact sachet is not required for reincubation.

7. After incubation, the exhausted CampyGen™ Compact paper sachet and plastic pouch should be sterilized and discarded with the non-hazardous laboratory waste.

LIMITATIONS

It is essential that the gas generating paper sachet be placed and sealed in the jar within one minute of its removal from the outer foil sachet. Extended exposure to air will result in loss of reactivity and full microaerophilic conditions may not be achieved.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, slides, staining supplies, microscopes, incinerators, incubators, seal bars or clips, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Reaction
Campylobacter jejuni
ATCC ® 33291
Growth

PHYSICAL APPEARANCE

CampyGen™ Compact should appear as white paper sachets individually packaged within a foil pouch.

CampyGen™ and AnaeroGen™ Compact

CampyGen™ Compact Gas Generating System
(Cat. no. CN020C).

REFERENCES

1.Van Horn, K.G., et al. 1997. Journal of Clinical Microbiology ; Vol. 35, No. 8, p. 2170-2173. American Society for Microbiology.

2. Anderson, N.L., et al. Cumitech 3A; Quality Control and Quality Assurance Practices in Clinical Microbiology, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

3. Jorgensen., et al. Manual of Clinical Microbiology , 6th ed. American Society for Microbiology, Washington, D.C.

4. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology , C.V. Mosby Company, St. Louis, MO.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook , Vol. I & II. American Society for Microbiology, Washington, D.C.

6. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology , J.B. Lippincott Company, Philadelphia, PA.

6. Package Insert, Oxoid Ltd., Basingstoke, Hampshire, RG24 8PW, England. November, 1997.

This document is provided for general product information only. It does not replace the manufacturer's product insert. Always refer to the actual product insert for procedural use and for most recent product information.


ATCC is a registered trademark of the American Type Culture Collection.
CampyGen is a trademark of Oxoid Ltd., Basingstoke, Hampshire, England.


IFU-10318[A]