CAMPY THIOGLYCOLLATE

Cat. no. K128 Campy Thioglycollate, 16x100mm Tube, 6ml 20 tubes/box

INTENDED USE

Hardy Diagnostics Campy Thioglycollate is a selective medium used to aid in the isolation of Campylobacter species from stool specimens.

SUMMARY

Campylobacter jejuni has been isolated as the cause of bacterial enteritis. Campy Thioglycollate medium is used to aid in the isolation of Campylobacter species from stool specimens. Campy Thioglycollate contains a battery of antimicrobics that inhibit most normal flora which may overgrow Campylobacter species on conventional collection media.

FORMULA

Ingredients per liter of deionized water:*

Pancreatic Digest of Casein 17.0gm
Glucose 6.0gm
Papaic Digest of Soybean Meal 3.0gm
Sodium Chloride 2.5gm
Sodium Thioglycollate 0.5gm
Sodium Sulfite 0.1gm
Vancomycin 0.01gm
Trimethoprim 5.0mg
Amphotericin B 2.0mg
Polymyxin B 2,500U
Agar 1.6gm

Final pH 7.3 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance standards.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat and freezing.

PRECAUTIONS

PROCEDURE

1. Inoculate Campy Thioglycollate directly with a clinical specimen suspected of containing Campylobacter species.

2. Incubate aerobically for 8 to 12 hours at 4ºC.

3. Place one or two drops of the inoculated broth onto plated media appropriate for the cultivation of Campylobacter spp. (i.e., Campy CVA, Cat. no. A40).

4. Incubate at appropriate temperature and atmosphere. Most Campylobacter require a microaerophilic atmosphere.

5. Examine at 48-72 hours for the presence of colonies typical of Campylobacter.

INTERPRETATION OF RESULTS

The growth of normal flora will be inhibited, improving the isolation of Campylobacter species.

LIMITATIONS

Campy Thioglycollate is not intended to be used as a medium for identification of Campylobacter species. Subculture to solid media is required for isolation. Additional biochemical tests are required for complete identification.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Campylobacter jejuni ***
ATCC ® 33291
A 8-12hr 4°C Aerobic Growth upon subculture
Proteus mirabilis ***
ATCC ® 12453
B 8-12hr 4°C Aerobic Partial to complete inhibition upon subculture
Staphylococcus aureus ***
ATCC ® 25923
B 8-12hr 4°C Aerobic Partial to complete inhibition upon subculture

*** Recommended QC strains for User Quality Control according to the CLSI document M22 when applicable.

Note: Campy Thioglycollate is inoculated with organism, incubated for 8-12 hours, then subcultured to a Campy CVA Agar plate, or similar Campy media. The Campy CVA Agar plate should show heavy growth of Campylobacter after 48-72 hours. P. mirabilis and S. aureus will show partial to complete inhibition.

User Quality Control

PHYSICAL APPEARANCE

Campy Thioglycollate should appear clear, and medium amber in color.

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Blaser, Martin, et al. 1978. Campylobacter enteritis associated with canine infection. The Lancet.; 2:979-980.


ATCC is a registered trademark of the American Type Culture Collection.

121415hh