CELLULOSE AGAR, MODIFIED

Cat. no. W83 Cellulose Agar, Modified, 15x100mm Plate, 28ml 10 plates/bag

INTENDED USE

Hardy Diagnostics Cellulose Agar, Modified is recommended for use as a general all-purpose growth media for the cultivation of cellulose-utilizing fungi from environmental sources.

This product is not intended to be used for the diagnosis of human disease.

SUMMARY

The genus Chaetomium contains a wide variety of fungal species found worldwide on substrates containing cellulose, including paper and plant compost materials. Chaetomium spp. often thrive in the presence of other fungal species that digest cellulose and require a high water content (e.g. Stachybotrys, Trichoderma, Acremonium, among others). These fungi dissolve cellulose fibers found in cotton and paper, causing the materials to disintegrate. This process is accelerated under moist or damp conditions. Consequently, Chaetomium have been recovered from indoor environmental surfaces: bathrooms, kitchens, wood, wallboard, dry wall, carpet and window frames. They are commonly found in dwellings and buildings with chronic water intrusion and are considered to be an indication of indoor environmental mold infestation.

Recent medical evidence suggests that individuals exposed to Chaetomium may be predisposed to neurological damage of the myelin sheath, autoimmune disorders like multiple sclerosis and lupus, as well as certain types of cancer. Moreover, Chaetomium is the only mold known to cause permanent DNA damage and inhibit cellular replication. Some species of Chaetomium (e.g. C. globosum and C. atrobrenneum) have been implicated in fatal infectious mycoses. C. globosum has also been known to cause onychomycosis, peritonitis, and cutaneous lesions. Therefore, the genus Chaetomium may be considered a potential pathogen when present in indoor environmental samples.

Hardy Diagnostics Cellulose Agar, Modified is an enrichment medium designed for the cultivation of cellulose-utilizing fungi from environmental samples. Cellulose is added as a carbon source. L-aspargine is added as a nitrogen source. Yeast extract not only provides vitamins and co-factors required for growth, but also serves as an additional source of nitrogen and carbon.

FORMULA

Ingredients per liter of deionized water:*

Cellulose 20.0gm
Potassium Phosphate 1.0gm
Ammonium Sulfate 0.5gm
L-Asparagine 0.5gm
Potassium Chloride 0.5gm
Yeast Extract 0.5gm
Magnesium Sulfate 0.2gm
Calcium Chloride 0.1gm
Agar 15.0gm

Final pH 7.0 +/- 0.3 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

PROCEDURE

Inoculate Hardy Diagnostics Cellulose Agar, Modified as per the techniques and procedures established by laboratory policy. Incubate media for up to 14 days at 15-30ºC.

INTERPRETATION OF RESULTS

Chaetomium colonies are rapidly growing, cottony and white in color initially. Mature colonies become gray to olive in color. From the reverse, the color is tan to red or brown to black.

Stachybotrys produces cottony, rapidly growing colonies which mature in about 4 days. From both front and reverse, the color of the colony is white initially and turns to black by aging. Refer to standard mycology textbooks and other resources for complete inhibition.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, microscopes. stains, swabs, applicator sticks, other culture media, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Stachybotrys chartarum
ATCC® 9182
G up to 14 days 15-30°C Aerobic Growth; white sporulating colonies
Pseudomonas aeruginosa
ATCC® 27853
A up to 3 days 15-30°C Aerobic Partial to complete inhibition
Escherichia coli
ATCC® 25922
A up to 3 days 15-30°C Aerobic Partial to complete inhibition
Staphylococcus aureus
ATCC® 25923
A up to 3 days 15-30°C Aerobic Partial to complete inhibition

USER QUALITY CONTROL

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen, et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

5. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

6. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

ATCC is a registered trademark of the American Type Culture Collection.

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