COLUMBIA CNA AGAR
|Cat. no. A50||Columbia CNA Agar, 15x100mm Plate, 19ml||10 plates/bag|
|Cat. no. A50BX||Columbia CNA Agar, 15x100mm Plate, 19ml||100 plates/box|
|Cat. no. A129||Columbia CNA Agar, 86x128mm Omniplate, 30ml||10 plates/bag|
|Cat. no. GA50||
Columbia CNA Agar, 15x100mm Plate, 19ml
(reduced stacking ring)
|Cat. no. J52||
Columbia CNA / EMB Agar, Levine, 15x100mm Biplate,
|Cat. no. J62||
Columbia CNA / MacConkey Agar, 15x100mm Biplate,
|Cat. no. J66||
Bile Esculin Agar (BEA) with Azide / Columbia CNA Agar,
15x100mm Biplate, 10ml/10ml
|Cat. no. J116||Columbia CNA / BluEcoli™, 15x100mm Biplate, 10ml/10ml||10 plates/bag|
Hardy Diagnostics Columbia CNA Agar is recommended for use as a selective growth medium for the isolation and differentiation of gram-positive cocci from clinical and non-clinical specimens which contain mixed flora.
Cat. no. A129 is not intended to be used for the diagnosis of human disease.
Columbia Blood Agar was first described in 1966 by Ellner, Stoessel, Drakeford, and Vasi who incorporated animal derived peptone, enzymatic digests of casein, and defibrinated sheep blood into one medium. (3) It was found to be an improved form of Blood Agar, promoting both luxuriant and rapid growth, improved pigment production, typical colony morphology, and sharply defined hemolytic reactions. Ellner, et al., also described the use of nalidixic acid and colistin in Columbia Blood Agar. (3) Columbia CNA Agar was designed to suppress the growth of most gram-negative bacteria, including Klebsiella , Proteus , and Pseudomonas species from mixed flora specimens, thus isolating for gram-positive staphylococci and streptococci. (3)
Ingredients per liter of deionized water:*
|Pancreatic Digests of Casein||12.0gm|
|Peptic Digest of Animal Tissue||5.0gm|
Final pH 7.3 +/- 0.3 at 25ºC.
* Adjusted and/or supplemented as required to meet performance criteria.
STORAGE AND SHELF LIFE
Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), hemolysis, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.
For Cat. nos. A50, A50BX, GA50, J52, J62, J66, J116.
For Cat. no. A129.
Specimen Collection: Consult listed references for information on specimen collection. (1,2,4,6) Infectious material should be submitted directly to the laboratory without delay and protected from excessive heat and cold. If there is to be a delay in processing, the specimen should be inoculated onto an appropriate transport media and refrigerated until inoculation.
Method of Use: Allow the plates to warm to room temperature, and the agar surface to dry before inoculating. Inoculate and streak the specimen as soon as possible after collection. If the specimen to be cultured is on a swab, roll the swab over a small area of the agar surface. Streak for isolation with a sterile loop. Incubate plates aerobically, anaerobically, or under conditions of increased CO 2 (5-10%) at 35-37ºC. for 24 hours. Examine colonial morphology, characteristics, and hemolytic reactions.
INTERPRETATION OF RESULTS
Consult listed references for the identification of colony morphology and further biochemical tests required for identification. (1,2,4,6)
Some beta-hemolytic streptococci strains will develop green hemolytic zones on Columbia CNA Agar. It is recommended to subculture all such streptococci strains onto Blood Agar plates (Cat. no. A10) to verify this reaction.
For optimal performance, it is recommended to incubate plates under increased CO 2 or aerobic conditions. Atmosphere of incubation has been shown to influence hemolytic reactions of beta-hemolytic streptococci. (6)
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.
|Test Organisms||Inoculation Method*||Incubation||Results|
ATCC ® 19615
|A||24hr||35°C||CO 2 **||Growth; beta-hemolysis|
ATCC ® 6305
|A||24hr||35°C||CO 2 **||Growth; alpha-hemolysis|
ATCC ® 25923
|A||24hr||35°C||CO 2 **||Growth|
ATCC ® 12453
ATCC ® 27853
User Quality Control
** Atmosphere of incubation is enriched with 5-10% CO 2 .
Columbia CNA Agar should appear opaque, and cherry red in color.
Streptococcus pyogenes (ATCC ® 19615) colonies growing on Columbia CNA Agar (Cat. no. A50). Incubated in CO 2 for 24 hours at 35ºC.
Streptococcus pneumoniae (ATCC ® 6305) colonies growing on Columbia CNA Agar (Cat. no. A50). Incubated in CO 2 for 24 hours at 35ºC.
Staphylococcus aureus (ATCC ® 25923) colonies growing on Columbia CNA Agar (Cat. no. A50). Incubated aerobically for 24 hours at 35ºC.
Uninoculated plate of Columbia CNA Agar (Cat. no. A50).
1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.
2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.
3. Ellner, et al. 1966. Am. Journ. Clin. Path.; 45:502.
4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.
5. MacFaddin, J.F. Biochemical Tests for Identification of Medical Bacteria,, Lipincott Williams & Wilkins, Philadelphia, PA.
6. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.
7. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.
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