LB Broth Powder
|Cat. no. C6010||CulGenex™ LB Broth, 2L||50gm|
|Cat. no. C6011||CulGenex™ LB Broth||500gm|
|Cat. no. C6012||CulGenex™ LB Broth||2kg|
|Cat. no. C6013||CulGenex™ LB Broth||10kg|
|Cat. no. C6019||CulGenex™ LB Broth, 0.5L||6 pouches/pack|
Hardy Diagnostics CulGenex™ LB Broth is used for the maintenance and propagation of Escherichia coli used in molecular biology procedures.
This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.
LB, or "lysogeny broth", media formulations have been widely used for the cultivation of Escherichia coli since the 1950s, and have become an industry standard in molecular microbiology applications for the preparation of plasmid DNA and the growth of recombinant strains. (3,5-8) LB medium was originally formulated by Giuseppe Bertani and published in 1951 and has since been modified by Miller, Lennox and Luria: the formulations differ in the concentration of sodium chloride, which provides for greater selectivity. (3) LB Agar, Miller medium contains 10gm of sodium chloride; LB Agar, Lennox contains 5gm of sodium chloride; and Luria Agar, Miller contains 0.5gm of sodium chloride. (3,5-8) Low salt formulations, such as those by Lennox and Luria, are ideal for salt-sensitive applications.
Adapted by J.H. Miller, LB Agar is a nutritionally rich medium designed for the growth and culture of pure recombinant strains used in genomic testing. (8) Hardy Diagnostics CulGenex™ LB Agar media formulations are based on the original recipe by Miller and contain casein peptone and yeast extract for amino acids, vitamins and essential minerals. Sodium chloride provides sodium ions for transport and helps maintain osmotic balance.
|Gram weight per liter:||25.0gm/L|
Final pH 7.0 +/- 0.2 at 25ºC.
* Adjusted and/or supplemented as required to meet performance criteria.
STORAGE AND SHELF LIFE
Store the sealed container(s) containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Keep container tightly sealed. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original off-white to beige.
Store the prepared media at 2-8ºC.
METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA
1. Suspend 25.0gm of the dehydrated culture media in 1 liter of distilled or deionized water (12.5gm per 500ml).
2. Heat as needed to dissolve completely.
3. Dispense desired volume into container.
4. Autoclave at 121ºC. for 15 minutes.
PROCEDURE AND INTERPRETATION OF RESULTS
For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. CG51BX.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as autoclaves, incubators, etc., are not provided.
The following organism is routinely used for testing at Hardy Diagnostics:
|Test Organisms||Inoculation Method*||Incubation||Results|
ATCC ® 25922
User Quality Control
CulGenex™ LB Broth powder should appear homogeneous, free-flowing, and off-white to beige in color. The prepared media should appear clear to slightly opalescent, and light amber in color.
1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.
2. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl. 1994. Current Protocols in Molecular Biology . Vol. 1. Current Protocols, New York, N.Y.
3. Bertani, G. 1951. Studies on Lysogenesis: The Mode of Phage Liberation by Lysogenic Escherichia coli . J. Bacteriol. ; 62:293-300.
4. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.
5. Lennox, E.S. 1955. Transduction of Linked Genetic Characteristics of the Host by Bacteriophage P1. Virology ; 1:190.
6. Luria, S.E., and J.W. Burrous. 1957. Hybridization Between Escherichia coli and Shigella . J. Bacteriol. ; 74:461-476.
7. Luria, S.E., J.N. Adams, and R.C. Ting. 1960. Transduction of Lactose-Utilizing Ability Among Strain of E. coli and S. dysenteriae and the Properties of the Transducing Phage Particles. Virology ; 12:348-390.
8. Miller, J.H. 1972. Experiments in Molecular Genetics . Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
9. Sambrook and Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y.
ATCC is a registered trademark of the American Type Culture Collection.