CulGenex™

Molecular Grade Waters - Ultra Pure and DEPC Treated

Cat. no. CG480BX DEPC Treated, Molecular Grade Water 125ml Polycarbonate Bottle, 100ml 6 bottles/box
Cat. no. CG490BX Ultra Pure, Molecular Grade Water, 125ml Square PC Bottle, 100ml 6 bottles/box
Cat. no. CG492BX Ultra Pure, Molecular Grade Water, 1L Polycarbonate Bottle, 1000ml 10 bottles/box

INTENDED USE

Hardy Diagnostics CulGenex™ DEPC Treated, Molecular Grade Water is chemically treated for use in various applications in molecular biology, particularly for creating RNAse-free solutions or to rinse RNAses from labware. CulGenex™ Ultra Pure, Molecular Grade Water is suitable for use in molecular biology applications that require water free of DNAses, RNAses and proteases.

This product is not intended to be used for the diagnosis of human disease.

SUMMARY

Nucleases (RNAses and DNAses) and proteases are enzymes capable of degrading DNA, RNA and protein molecules. They are ubiquitous, environmentally stable and difficult to inactivate. The most common source of nuclease and protease contamination comes from skin and bacteria or mold that may be present on airborne dust particles or laboratory ware. The proper use of gloves and the use of DEPC Treated or Ultra Pure molecular grade waters can help eliminate nucleases and proteases on labware and in laboratory solutions.

Diethyl pyrocarbonate (DEPC) is an efficient, nonspecific RNAase inhibitor. It is typically used at a concentration of 0.1% to treat water used to prepare nuclease-free laboratory solutions before working with sensitive molecules such as RNA, or for PCR applications. DEPC reacts with amine, hydroxly and thiol groups of proteins, and inactivates nucleases and other enzymes by covalent modification.

Ultra Pure, Molecular Grade Water is DNAse, RNAse and protease-free. It is made without the use of toxic agents, thus eliminating any potential interference of enzymatic reactions, and is sterile-filtered. It is suitable for use in all molecular biology procedures that demand a high quality of water purity, including PCR applications.

The source water used to prepare Hardy Diagnostics CulGenex™ DEPC Treated and Ultra Pure Molecular Grade Waters is Type 1 >18 megohms*, deionized and processed by reverse osmosis. The water has been treated by UV light and filtered through a 0.1um filter. Hardy Diagnostics CulGenex™ DEPC Treated and Ultra Pure Molecular Grade Waters are ready to use. They are sold by the individual bottle or by the package.

*Refer to water report for specific testing specifications.

FORMULA

Ingredients per liter:*
CulGenex™ DEPC Treated, Molecular Grade Water (Cat. no. CG480BX):
Molecular Grade Water, >18 megohms 1000.0ml
Diethyl Pyrocarbonate (DEPC) 1.0ml

Final pH 6.0 +/- 1.0 at 25ºC.

Ingredients per liter:*
CulGenex™ Ultra Pure, Molecular Grade Water (Cat. no. CG490BX, CG492BX):
Molecular Grade Water, >18 megohms 1000.0ml

Final pH 6.0 +/- 1.0 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-30ºC. away from direct light. Product should not be used if there are any signs of deterioration, discoloration, contamination, or if the expiration date has passed.


PRECAUTIONS


PROCEDURE

Consult additional references for information on specific standard protocols. (1-5)

LIMITATIONS

DEPC is highly unstable in the presence of Tris buffers and decomposes rapidly into ethanol and CO 2 .

Trace amounts of DEPC will modify purine residues in RNA by carboxymethylation. Carboxymethylated RNA is translated with very low efficiency in cell-free systems. Yet, its ability to form DNA:RNA or RNA:RNA hybrids is not seriously affected unless a large fraction of the purine residues have been modified.


QUALITY CONTROL

Hardy Diagnostics tests CulGenex™ DEPC Treated, Molecular Grade Water and CulGenex™ Ultra Pure, Molecular Grade Water for sterility, endotoxin, nuclease activity, protease activity, pH, and fill volume.

USER QUALITY CONTROL


Physical Appearance

CulGenex™ DEPC Treated, Molecular Grade Water and CulGenex™ Ultra Pure, Molecular Grade Water should appear clear, and colorless.

REFERENCES

1. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl, Editors. 2010. Current Protocols in Molecular Biology . John Wiley and Sons, Inc. Malden, MA.

2. Bullock, G.R. and Petrusz, P. 1989. Techniques in Immunocytochemistry, Volumes 1, 2, 3 and 4, Academic Press, London.

3. Cseke, L.J., P.B. Kaufman, G.K. Podila, and C.J. Tsai. 2004. Handbook of Molecular and Cellular Methods in Biology and Medicine . CRC Press.Taylor & Francis LLC. Boca Raton, FL.

4. Sambrook and Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press. Woodbury, New York.

5. Walker, J.M. 1984. Methods in Molecular Biology . The Humana Press Inc. Clifton, NJ.


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