CulGenex™

Phosphate Buffered Saline

Cat. no. CG182BX Phosphate Buffered Saline (PBS), 1X, pH 7.4, 1L Polycarbonate Bottle, 1000ml 10 bottles/box
Cat. no. CG202 Phosphate Buffered Saline (PBS), 10X, pH 7.4, 1L Polycarbonate Bottle, 1000ml 1 each
Cat. no. CG202BX Phosphate Buffered Saline (PBS), 10X, pH 7.4, 1L Polycarbonate Bottle, 1000ml 10 bottles/box
Cat. no. CG223 Phosphate Buffered Saline, Dulbecco's (DPBS), 10X,
pH 7.2-7.4, 1L Polycarbonate Bottle, 1000ml
1 each

INTENDED USE

Hardy Diagnostics CulGenex™ Phosphate Buffered Saline and Dulbecco's Phosphate Buffered Saline are recommended for use in various procedures used in biological and molecular research. (1-6)

This product is not intended to be used for the diagnosis of human disease.

SUMMARY

Commonly used in biological and biomedical research, Phosphate Buffered Saline (PBS) has many applications due to its isotonic nature. PBS can be used as a diluting agent in preparing decimal dilutions and as a rinsing agent for rinsing labware containing cells. Phosphate salts are nontoxic to living cells and have a high buffering capacity; pH maintenance is important in retaining cell viability and in the recovery and revitalization of injured or damaged cells during bacterial culture and analysis. Phosphate Buffered Saline contains sodium chloride, sodium phosphate, potassium phosphate and, in some formulations, potassium chloride, magnesium and calcium.

Dulbecco's Phosphate Buffered Saline (DPBS) is commonly used to provide a buffer system to maintain cell culture media in the physiological pH range of 7.0 to 7.6, where a chemically defined balanced salt solution is needed to maintain cell tonicity and viability in vitro . DPBS is also commonly used in cell enumerations as a diluting, irrigating or transporting fluid, and as a buffer in many chromotographic procedures. Hardy Diagnostics CulGenex™ DPBS buffer is available with or without the addition of calcium and magnesium ions.

Hardy Diagnostics CulGenex™ Phosphate Buffered Saline products are available by the bottle or a box of ten.

FORMULA

Ingredients per liter of molecular grade water:*
Phosphate Buffered Saline (PBS), 1X (Cat. no. CG182BX):
Sodium Chloride 9.0gm
Disodium Phosphate 0.795gm
Monopotassium Phosphate 0.144gm

Final pH 7.4 to 7.6 +/- 0.1 at 25°C.

Ingredients per liter of molecular grade water:*
Phosphate Buffered Saline (PBS), 10X (Cat. no. CG202, CG202BX):
Sodium Chloride 90.0gm
Disodium Phosphate 7.95gm
Monopotassium Phosphate 1.44gm

Final pH 7.2 to 7.4 +/- 0.1 at 25°C.

Ingredients per liter of molecular grade water:*
Phosphate Buffered Saline, Dulbecco's (DPBS), 10X (Cat. no. CG223):
Sodium Chloride 80.0gm
Disodium Phosphate 21.6gm
Potassium Chloride 2.0gm
Monopotassium Phosphate 2.0gm

Final pH 7.2 to 7.4 +/- 0.2 at 25°C.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-30°C away from direct light. Buffers should not be used if there are any signs of deterioration, discoloration, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.


PRECAUTIONS


PROCEDURE

General Dilution Guidelines:

1:10 Serial Dilutions
1. Using a sterile pipet, aliquot 10ml of test suspension to 90ml of PBS diluent. Mix thoroughly. This yields a 1:10 dilution.

2. Use a second sterile pipet to aliquot 10ml of the 1:10 dilution prepared in step 1 into a second 90ml filled vessel of PBS diluent. Mix thoroughly. This yields a 1:100 dilution.

3. Continue aliquoting 10ml of subsequent dilutions into 90ml filled PBS diluent vessels until the desired concentration of test sample is obtained. Each succeeding dilution increases by a factor of 10. A separate sterile pipet should be used with each dilution.

1:100 Serial Dilutions
1. Using a sterile pipet, aliquot 1ml of test suspension to 99ml of PBS diluent. Mix thoroughly. This yields a 1:100 dilution.

2. Use a second sterile pipet to aliquot 1ml of the 1:100 dilution prepared in step 1a into a second 99ml filled vessel of PBS diluent. Mix thoroughly. This yields a 1:10,000 dilution.

3. Continue aliquoting 1ml of subsequent dilutions into 99ml filled PBS diluent vessels until the desired concentration of test sample is obtained. Each succeeding dilution increases by a factor of 100. A separate sterile pipet should be used with each dilution.

Consult additional references for information on specific standard protocols. (1-6)

LIMITATIONS

Note: PBS containing calcium and magnesium is not recommended for enzymatic reactions due to its potential interaction with divalent cations and resulting precipitation.


QUALITY CONTROL


USER QUALITY CONTROL

Check for signs of contamination and deterioration. Users of commercially prepared culture media may be required to perform quality control testing to demonstrate growth or a positive reaction and to demonstrate inhibition or a negative reaction (where applicable). Refer to the following documents on the Hardy Diagnostics Technical Document website for more information on QC: " Introduction to Quality Control ," " Finished Product Quality Control Procedures ," or " The CLSI Standard and Recommendations for User Quality Control of Media ."

Physical Appearance

CulGenex™ Phosphate Buffered Saline (PBS), 1X; Phosphate Buffered Saline (PBS), 10X; and Phosphate Buffered Saline, Dulbecco's (DPBS), 10X should appear clear, and colorless.

REFERENCES

1. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl, Editors. Current Protocols in Molecular Biology . John Wiley and Sons, Inc. Malden, MA.

2. Bullock, G.R. and Petrusz, P. Techniques in Immunocytochemistry, Volumes 1, 2, 3 and 4, Academic Press, London.

3. Cseke, L.J., P.B. Kaufman, G.K. Podila, and C.J. Tsai. Handbook of Molecular and Cellular Methods in Biology and Medicine . CRC Press. Taylor & Francis LLC. Boca Raton, FL.

4.Versalovic, J., et al. Manual of Clinical Microbiology. American Society for Microbiology, Washington, D.C.

5. Sambrook and Russell. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. Woodbury, New York.

6. Walker, J.M. Methods in Molecular Biology . The Humana Press Inc. Clifton, NJ.


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