CulGenex™

Yeast Peptone Dextrose (YPD) Powders

Cat. no. C1149 CulGenex™ Yeast Peptone Dextrose (YPD) Agar, 0.5L 6 pouches/pack
Cat. no. C1159 CulGenex™ Yeast Peptone Dextrose (YPD) Broth, 0.5L 6 pouches/pack

INTENDED USE

Hardy Diagnostics CulGenex™ Yeast Peptone Dextrose (YPD) Powders are recommended for the rapid growth of yeasts, particularly Saccharomyces cerevisiae , used for molecular research.

This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements.

SUMMARY

The genome of Saccharomyces cerevisiae can be easily manipulated and is recognized as a model test organism for diversified biological study. Because the genome of S. cerevisiae was the first eukaryotic genome to be sequenced, it became a key organism for genetic research. Typical studies include DNA microarray, gene function by disruption analysis, serial analysis of gene expression (SAGE), protein localization, mapping, and analysis of enzyme and lethality functions. (1,4-6) Many human genes related to disease have orthologs in yeast, and the conservation of metabolic and regulatory mechanisms in eukaryotes has contributed to the wide-spread use of yeast as a model test organism. In addition, the ability of yeast to replicate yeast artificial chromosomes (YACs) has yielded detailed studies on chromosomal mutations and origins of replication. (1,5)

Yeast grow best on minimal media containing only dextrose and salts: adding protein and yeast cell extract hydrolysates helps to promote more rapid growth and cell division. CulGenex™ Yeast Peptone Dextrose (YPD) Powders contain yeast extract, peptone and dextrose (D-glucose) and can be utilized in both liquid (broth) and solid (agar) forms; yeast extract and peptone provide carbon, nitrogen, amino acids, essential minerals, vitamins and trace elements to promote growth; dextrose is the energy source, agar, when applicable, is the solidifying agent.

Hardy Diagnostics CulGenex™ Yeast Peptone Dextrose (YPD) Powders are ready to use and come packaged in a convenient pouch to yield 500ml of media. Pouches are available as a package of six.

FORMULA*

Yeast Peptone Dextrose Broth Powder
Gram weight per liter: 50.0gm/L
Peptone 20.0gm
Dextrose (D-Glucose) 20.0gm
Yeast Extract 10.0gm

Final pH 6.5 +/- 0.2 at 25ºC.


In addition, Yeast Peptone Dextrose Agar Powder contains:

Gram weight per liter: 70.0gm/L
Agar 20.0gm

Final pH 6.5 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Store the sealed pouches containing dehydrated culture medium at 2-30ºC. Dehydrated culture medium is very hygroscopic. Protect dehydrated culture media from moisture and light. The dehydrated culture media should be discarded if it is not free-flowing or if the color has changed from its original off-white.

Store the prepared culture media at 2-8ºC.


PRECAUTIONS


METHOD OF PREPARATION FOR DEHYDRATED CULTURE MEDIA

1. Suspend the dehydrated powder (50.0gm broth powder or 70.0gm agar powder) in one liter of distilled or deionized water. Stir to mix thoroughly.

2. Heat broth powder as necessary to dissolve completely. Heat agar powder to boiling for approximately 1 minute to dissolve completely.

3. Sterilize in the autoclave at 121ºC. for 15 minutes.

4. Cool to 45-50ºC.

PROCEDURE AND INTERPRETATION OF RESULTS

For information on procedures and interpretation of results, consult listed references or refer to the prepared media Instructions for Use (IFU) for Cat. No. G422.

LIMITATIONS

YPD media are nonselective and cannot be used as a selective medium to test for auxotrophs.


MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as autoclaves, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Hardy Diagnostics tests each lot of commercially manufactured media using appropriate quality control microorganisms and quality specifications as outlined on the Certificates of Analysis (CofA).

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Saccharomyces cerevisiae
ATCC ® 9763
A 1-3 days 30°C Aerobic Growth

User Quality Control


Physical Appearance

CulGenex™ Yeast Peptone Dextrose (YPD) Media powder should appear homogeneous, free-flowing, and off-white in color. The prepared media should appear clear to translucent, and light amber in color.

REFERENCES

1. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl, Editors. 2010. Current Protocols in Molecular Biology . John Wiley and Sons, Inc. Malden, MA.

2. Cseke, L.J., P.B. Kaufman, G.K. Podila, and C.J. Tsai. 2004. Handbook of Molecular and Cellular Methods in Biology and Medicine . CRC Press.Taylor & Francis LLC. Boca Raton, FL.

3. Fowell, R.R. 1952. Sodium acetate agar as a sporulation medium for yeast. Nature (London) ; 170:578.

4. Sambrook and Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press. Woodbury, New York.

5. Sherman, F. 2002. Getting started with yeast. Meth. Enzymol ; 350:3-41.

6. Sherman, F., G.R. Fink, J.B. Hicks. 1986. The Laboratory Course Manual for Methods in Yeast Genetics . Cold Spring Harbor Press. Cold Spring Harbor, NY.

7. Walker, J.M. 1984. Methods in Molecular Biology . The Humana Press Inc. Clifton, NJ.


ATCC is a registered trademark of the American Type Culture Collection.

052016sw