CulGenex™

Yeast peptone Dextrose (YPD) Media

Cat. no. CG91 Yeast Peptone Dextrose (YPD) Broth, 500ml Polycarbonate Bottle, 500ml 1 each
Cat. no. G422 Yeast Peptone Dextrose (YPD) Agar, 15x100mm Plate, 26ml 10 plates/bag

INTENDED USE

Hardy Diagnostics CulGenex™ Yeast Peptone Dextrose (YPD) Media are recommended for the rapid growth of yeasts, particularly Saccharomyces cerevisiae , used for molecular research.

This product is not intended to be used for the diagnosis of human disease.

SUMMARY

The genome of Saccharomyces cerevisiae can be easily manipulated and is recognized as a model test organism for diversified biological study. Because the genome of S. cerevisiae was the first eukaryotic genome to be sequenced, it became a key organism for genetic research. Typical studies include DNA microarray, gene function by disruption analysis, serial analysis of gene expression (SAGE), protein localization, mapping, and analysis of enzyme and lethality functions. (1,4-6) Many human genes related to disease have orthologs in yeast, and the conservation of metabolic and regulatory mechanisms in eukaryotes has contributed to the wide-spread use of yeast as a model test organism. In addition, the ability of yeast to replicate yeast artificial chromosomes (YACs) has yielded detailed studies on chromosomal mutations and origins of replication. (1,5)

Yeast grow best on minimal media containing only dextrose and salts: adding protein and yeast cell extract hydrolysates helps to promote more rapid growth and cell division. CulGenex™ Yeast Peptone Dextrose (YPD) Media contain yeast extract, peptone and dextrose (D-glucose) and can be utilized in both liquid (broth) and solid (agar) forms; yeast extract and peptone provide carbon, nitrogen, amino acids, essential minerals, vitamins and trace elements to promote growth; dextrose is the energy source, agar, when applicable, is the solidifying agent.

Hardy Diagnostics CulGenex™ Yeast Peptone Dextrose (YPD) Media are pre-made and ready to use. They are available in a variety of packaging styles.

FORMULA

Ingredients per liter of deionized water:*

Yeast Peptone Dextrose Broth (Cat. no. CG91):
Peptone 20.0gm
Dextrose (D-Glucose) 20.0gm
Yeast Extract 10.0gm

Final pH 6.5 +/- 0.2 at 25ºC.


In addition, Yeast Peptone Dextrose Agar (Cat. no. G422) contains:

Agar 20.0gm

Final pH 6.5 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store media at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing. Additionally, agar media should not be used is there are any signs of shrinking, cracking, or discoloration.


PRECAUTIONS


PROCEDURE

Consult listed references for information on specific protocols. (1-7)

INTERPRETATION OF RESULTS

Development of colonies on the solid agar or turbidity in the broth are indicative of growth.

LIMITATIONS

YPD media are nonselective and cannot be used as a selective medium to test for auxotrophs.


MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, swabs, applicator sticks, other culture media, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Hardy Diagnostics tests each lot of commercially manufactured media using appropriate quality control microorganisms and quality specifications as outlined on the Certificates of Analysis (CofA).

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Saccharomyces cerevisiae
ATCC ® 9763
A 1-3 days 30°C Aerobic Growth

USER QUALITY CONTROL


Physical Appearance

Yeast Peptone Dextrose (YPD) Agar should appear translucent, and light amber in color.
Yeast Peptone Dextrose (YPD) Broth should appear clear, and light amber in color.

REFERENCES

1. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl, Editors. 2010. Current Protocols in Molecular Biology . John Wiley and Sons, Inc. Malden, MA.

2. Cseke, L.J., P.B. Kaufman, G.K. Podila, and C.J. Tsai. 2004. Handbook of Molecular and Cellular Methods in Biology and Medicine . CRC Press.Taylor & Francis LLC. Boca Raton, FL.

3. Fowell, R.R. 1952. Sodium Acetate Agar as a Sporulation Medium for Yeast. Nature (London) ; 170:578.

4. Sambrook and Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press. Woodbury, New York.

5. Sherman, F. 2002. Getting Started with Yeast. Meth. Enzymol ; 350:3-41.

6. Sherman, F., G.R. Fink, J.B. Hicks. 1986. The Laboratory Course Manual for Methods in Yeast Genetics . Cold Spring Harbor Press. Cold Spring Harbor, NY.

7. Walker, J.M. 1984. Methods in Molecular Biology . The Humana Press Inc. Clifton, NJ.


ATCC is a registered trademark of the American Type Culture Collection.

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