CZAPEK-DOX AGAR

Cat. no. W119 Czapek-Dox Agar, 15x100mm Plate, 28ml 10 plates/bag

INTENDED USE

Hardy Diagnostics Czapek-Dox Agar is recommended for use in cultivating fungi and bacteria capable of using inorganic nitrogen.

SUMMARY

Czapek-Dox Agar is a modification of the Czapek (1902-1903) and Dox (1910) formula prepared according to Thom and Church. The medium contains sucrose as the sole source of cabon and nitrate as the only inorganic source of nitrogen. The medium is useful in a variety of microbiological procedures, including fungi and mildew resistance tests and soil microbiology testing. Czapek-Dox Agar produces luxuriant growth of most saprophytic aspergilli causing the organisms to produce characteristic mycelia and conidia.

Czapek-Dox Agar is recommended in Standard Methods for the Examination of Water and Wastewater for the isolation of Aspergillus, Penicillium, Paecilomyces and other types of fungi with similar physiological requirements.

FORMULA

Ingredients per liter of deionized water:*

Sucrose 30.0gm
Sodium Nitrate 2.0gm
Dipotassium Phosphate 1.0gm
Magnesium Sulfate 0.5gm
Potassium Chloride 0.5gm
Ferrous Sulfate 0.01gm
Agar 15.0gm

Final pH 7.3 +/- 0.3 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

PROCEDURE

1. Inoculate by spreading the sample material thinly on the surface of the culture medium.

2. Incubate for up to 1 week at 28ºC. Optimal incubation temperatures are as follows:

Penicillium spp. at 20-25ºC.
Aspergillus spp. at 30ºC.
Candida spp. at 28ºC.

Refer to appropriate references for specific procedures for the cultivation of fungi and bacteria capable of metabolizing inorganic nitrogen. (1)

INTERPRETATION OF RESULTS

Consult reference for interpretation. (1)

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, swabs, applicator sticks, other culture media, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Aspergillus brasiliensis
formerly A. niger
ATCC® 16404

G 48-72hr 15-30°C Aerobic Growth
Candida albicans
ATCC® 10231
A 48-72hr 15-30°C Aerobic Growth
Candida albicans
ATCC® 60193
A 48-72hr 15-30°C Aerobic Growth

USER QUALITY CONTROL

Physical Appearance

Czapek-Dox Agar should appear slightly opalescent, and white to light amber in color; may have a slight precipitate.

REFERENCES

1. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.

2. Hurst, C.J., et al. 2002. Manual of Environmental Microbiology, 2nd ed. ASM Press, Washington D.C.

3. St-Germain, G., et al. 1996. Identifying Filamentous Fungi A Clinical Laboratory Handbook, Star Publishing Company, Belmont, CA.

ATCC is a registered trademark of the American Type Culture Collection.

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