Cat. no. SRK45 EnviroTrans™ D/E Neutralizing Broth, 15x75mm Polyproplyene Tube with swab, 5ml 20 vials/box


Hardy Diagnostics EnviroTrans™ D/E Neutralizing Broth is designed for sampling surfaces as a part of HACCP (Hazard Analysis Critical Control Point) systems and other environmental monitoring testing. The EnviroTrans™ D/E Neutralizing Broth packages everything needed to collect samples from work surfaces, utensils or other industrial apparatuses (i.e. pump impellers, gaskets, etc.).

This product is not intended to be used for the diagnosis of human disease.


Hardy Diagnostics EnviroTrans™ D/E Neutralizing Broth is a self contained unit which includes a sterile dacron swab, attached to the lid, and a tube containing 5ml of D/E Neutralizing Broth.

Quaternary ammonium compounds are molecules which contain a nitrogen atom with four other atoms bonded to it. Most quaternary ammonium compounds are organic compounds and have biological activity. These compounds often work well as disinfectants, offering bactericidal and bacteriostatic effects.

D/E Neutralizing Broth, as the transport media in this unit, has the ability to neutralize a broad spectrum of antiseptic and disinfectant chemicals including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde and glutaraldehyde (see Chart 1 ). It is intended to facilitate survival of organisms in the sample during transport to the lab.


Ingredients per liter of deionized water:*

Dextrose 10.0gm
Lecithin 7.0gm
Sodium Thiosulfate 6.0gm
Tween ® 80 5.0gm
Pancreatic Digest of Casein 5.0gm
Dipotassium Phosphate 3.3gm
Sodium Bisulfite 2.5gm
Yeast Extract 2.5gm
Sodium Thioglycollate 1.0gm
Monopotassium Phosphate 0.1gm
Bromcresol Purple 20.0mg

Final pH 7.6 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.


Storage: Upon receipt, store at 2-8ºC. Always keep away from direct light. Media should not be used if there are any signs of deterioration, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat and freezing.



Specimen Collection (1)

Aseptically collect sample. Collect sample by rubbing the swab over the sample area (approximately 50cm 2 ), reversing directions between strokes. Repeat the collection procedure three more times, returning the swab head to the D/E Neutralizing Broth after swabbing each area. When sampling utensils such as knives or ladles, run the swab over the entire surface of the instrument three times, as described above. If sample is not immediately taken to the lab the sample can be refrigerated for up to 24 hours prior to analysis.