Cat. no. SRK25 EnviroTrans™ Letheen Broth, 15x75mm Polyproplyene Tube with swab, 5ml 20 vials/box


Hardy Diagnostics EnviroTrans™ Letheen Broth is designed for sampling surfaces as a part of HACCP (Hazard Analysis Critical Control Point) systems and other environmental monitoring. The EnviroTrans™ Letheen Broth packages everything needed to collect samples from work surfaces, utensils or other industrial apparatuses (i.e. pump impellers, gaskets, etc.).

This product is not intended to be used for the diagnosis of human disease.


Hardy Diagnostics EnviroTrans™ Letheen Broth is a self contained unit which includes a sterile dacron swab, attached to the lid, and a tube containing 5ml of Letheen Broth.

Quaternary ammonium compounds are molecules which contain a nitrogen atom with four other atoms bonded to it. Most quaternary ammonium compounds are organic compounds and have biological activity. These compounds often work well as disinfectants, offering bactericidal and bacteriostatic effects. Letheen Broth is used in the AOAC procedure for determining the effect of these bactericidal compounds. (3)

Letheen Broth, the transport media in this unit, is formulated according to the guidelines set by the AOAC for the determination of phenol coefficient of quaternary ammonium compounds. (3) The medium contains beef extract and peptic digest of animal tissue to provide a nutrient rich medium supporting the growth of a variety of microorganisms. Quaternary ammonia compounds are neutralized by lecithin while phenolic disinfectants and hexachlorophene are neutralized by Tween® 80. Together, lecithin and Tween® 80 neutralize ethanol.


Ingredients per liter of deionized water:*

Peptic Digest of Animal Tissue 10.0gm
Beef Extract 5.0gm
Sodium Chloride 5.0gm
Tween® 80 5.0gm
Lecithin 0.7gm

Final pH 7.0 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.


Storage: Upon receipt, store at 2-8ºC. Always keep away from direct light. Media should not be used if there are any signs of deterioration, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat and freezing.



Specimen Collection (1)

Aseptically collect sample. After removing the swab from its wrapper, moisten the tip in the Letheen Broth. Collect sample by rubbing the swab over the sample area (approximately 50cm 2 ), reversing directions between strokes. Repeat the collection procedure three more times, returning the swab head to the Letheen Broth after swabbing each area. When sampling utensils such as knives or ladles, run the swab over the entire surface of the instrument three times, as described above. If sample is not immediately taken to the lab, the sample can be refrigerated for up to 24 hours prior to analysis.