FDA RECOMMENDED MEDIA FOR THE TESTING OF FOODS

The following list of media is recommended by the FDA for use in the testing of foods.(1)

FDA No. Medium Name Prepared Media Catalog No. CRITERION™ Media Catalog No.
M1 A-1 Broth   C7570, C7571, C7572, C7573, C7574
M29a Abeyta-Hunt-Bark Agar    
M2 Acetamide Agar L02  
M2 Acetamide Broth    
M3 Acetate Agar L15  
M4 Acid Broth    
M5 AE Sporulation Medium, Modified
(for C. perfringens)
   
M6 Agar Medium P   C5000, C5001, C5002, C5003, C5004
M7 AKI Medium    
M8 Alkaline Peptone Agar    
M9 Alkaline Peptone Salt Broth    
M10 Alkaline Peptone Water K03  
M10a ALOA (Agar Listeria Ottavani & Agosti) Medium    
M11 Anaerobe Agar    
M12 Anaerobic Egg Yolk Agar AG401  
M13 Andrade's Carbohydrate Broth and Indicator Y79-Y95  
M14 Antibiotic Medium No. 1 (Agar Medium A)    
M15 Antibiotic Medium No. 4 (Agar Medium B)    
M16 Arginine-Glucose Slant (AGS)    
M17 Baird-Parker Medium, pH 7.0 G96, P16 C5080, C5081, C5082, C5083, C5084
M18 Bile Esculin Agar G12, L10 C5180, C5181, C5182, C5183, C5184
M17a Biosynth® Chromogenic Medium (BCM®)
for Listeria monocytogenes
   
FDA No. Medium Name Prepared Media Catalog No. CRITERION™ Media Catalog No.
M19 Bismuth Sulfite Agar (Wilson and Blair)   C5210, C5211, C5212, C5213, C5214
M20 Blood Agar A10, L12, P33  
M20a Blood Agar Base   C5220, C5221, C5222, C5223, C5224
M21 Blood Agar Base (Infusion Agar)   C5820, C5821, C5822, C5823, C5824
M22 Blood Agar No. 2    
M23 Brain Heart Infusion (BHI) Agar (0.7%)
(for staphylococcal enterotoxin)
   
M24 Brain Heart Infusion Agar L35, W15, X10 C5120, C5121, C5122, C5123, C5124
M24 Brain Heart Infusion Broth R10, R15, R20, K27 C5140, C5141, C5142, C5143, C5144
M25 Brilliant Green Lactose Bile Broth K09  
M26 Bromcresol Purple Broth    
M27 Bromcresol Purple Dextrose Broth (BCP)    
M52 Buffered Listeria Enrichment Broth,
pH 7.3 +/- 0.1
U167  
M192 Buffered Peptone Water (BPW) K107, K195 C5320, C5321, C5322, C5323, C5324
M28a Campylobacter Enrichment Broth
(Bolton formula)
U83  
M31 Cary and Blair Transport Medium    
M32 Casamino Acid Yeast Extract Broth
(CYE Broth)
   
M34 Casamino Acids Yeast Extract Salts (CA-YE) Broth (Gorbach)    
M35 CIN Agar G20 C5390, C5391, C5392, C5393, C5394
M36 Cell Growth Medium    
M189 Cellulase Solution    
M187 Cellobiose-Colistin (CC) Agar    
M37 Cetrimide Agar G18 C5370, C5371, C5372, C5373, C5374
M38 Chopped Liver Broth    
M39 Christensen Citrate Agar    
M40 Christensen's Urea Agar L65, R42 C7230, C7231, C7232, C7233, C7234
M40a CHROMagar™ Listeria    
M40b Chromogenic Listeria Agar    
M41 Congo Red BHI Agarose (CRBHO) Medium*    
FDA No. Medium Name Prepared Media Catalog No. CRITERION™ Media Catalog No.
M42 Cooked Meat Medium K219 C5480, C5481, C5482, C5483, C5484
M43 Cooked Meat Medium, Modified    
M44 Decarboxylase Medium Base, Falkow    
M193 Dey-Engley Broth    
M184 Dichloran 18% Glycerol (DG18) Agar W85  
M183 Dichloran Rose Bengal Chloramphenicol (DRBC) Agar    
M45 Duncan-Strong (DS) Sporulation Medium, Modified (for C. perfringens)    
M46 Eagle's Minimal Essential Medium (MEME)
(with Earle's salts and nonessential amino acids)
   
M47 Earle's Balanced Salts (Phenol Red-Free)    
M48 EB Motility Medium    
M49 EC Broth K13, K63 C5690, C5691, C5692, C5693, C5694
M50 EC-MUG Medium K18, K64 C5680, C5681, C5682, C5683, C5684
M51 Egg Yolk Emulsion, 50%    
M53 Esculin Agar, Modified (CDC) L29  
M30b Freezing Medium    
M54 Gelatin Agar (GA)    
M55 Gelatin Salt Agar (GS)    
M57 Gentamicin Sulfate Solution    
M58 Ham's F-10 Medium    
M59 Heart Infusion Agar (HIA)   C5820, C5821, C5822, C5823, C5824
M60 Heart Infusion Agar (HIA) (for Vibrio)    
M60 Heart Infusion (HI) Broth (for Vibrio)    
M61 Hektoen Enteric (HE) Agar G63 C5840, C5841, C5842, C5843, C5844
M62 HC (Hemorrhagic colitis E. coli strains) Agar    
M63 Hugh-Leifson's Glucose Broth (HLGB)    
M64 Indole Medium    
M65 Indole Medium (CDC)    
M66 Indole Nitrate Medium (Tryptic Nitrate) K147 C5870, C5871, C5872, C5873, C5874
M67 Irgasan®-Ticarcillin-Chlorate (ITC) Broth    
M68 Iron Milk Medium (Modified)    
M69 King's B Medium    
FDA No. Medium Name Prepared Media Catalog No. CRITERION™ Media Catalog No.
M70 King's O/F Basal Medium Y51 C6500, C6501, C6502, C6503, C6504
M71 Kliger Iron Agar L70, R70 C5910, C5911, C5912, C5913, C5914
M72 Koser's Citrate Broth    
M73 L-15 Medium (Modified) Leibovitz    
M74 Lactose Broth U92 C5940, C5941, C5942, C5943, C5944
M75 Lactose-Gelatin Medium (for C. perfringens)    
M76 Lauryl Tryptose (LST) Broth K32 C5980, C5981, C5982, C5983, C5984
M77 Lauryl Tryptose MUG (LST-MUG) Broth K35 C5990, C5991, C5992, C5993, C5994
M78 Letheen Agar (Modified) G221 C7900, C7901, C7902, C7903, C7904
M79 Letheen Broth (Modified)   C7640, C7641, C7642, C7643, C7644
M80 Levine's Eosin-Methylene Blue (L-EMB) Agar G25, P09 C5700, C5701, C5702, C5703, C5704
M81 Lithium Chloride-Phenylethanol-Moxalactam (LPM) Medium G92 C6060, C6061, C6062, C6063, C6064
M82 [Lithium Chloride-Phenylethanol-Moxalactam] LPM Plus Esculin and Ferric Iron    
M83 Liver-Veal Agar    
M84 Liver-Veal-Egg Yolk Agar    
M85 Long-Term Preservation Medium    
M86 Lysine Arginine Iron Agar (LAIA)    
M87 Lysine Decarboxylase Broth (Falkow)
(for Salmonella)
   
M88 Lysine Decarboxylase (LDC) Medium
(for Gram-negative nonfermentative bacteria)
   
M89 Lysine Iron Agar (Edwards and Fife) L25, R22  
M90 Lysozyme Broth    
M91 MacConkey Agar G35 C6130, C6131, C6132, C6133, C6134
M92 Malonate Broth K36  
M185 Malt Agar (MA)    
M93 Malt Extract Agar
(Cosmetics-General Microbiology)
P93, W28 C6200, C6201, C6202, C6203, C6204
M182 Malt Extract Agar (Yeasts and Molds) (MEAYM)    
M94 Malt Extract Broth   C6210, C6211, C6212, C6213, C6214
FDA No. Medium Name Prepared Media Catalog No. CRITERION™ Media Catalog No.
M95 Mannitol-Egg Yolk-Polymyxin (MYP) Agar G147 C7400, C7401, C7402, C7403, C7404
M96 Mannitol Maltose Agar    
M97 Mannitol Salt Agar G40, L32, P98 C6230, C6231, C6232, C6233, C6234
M30a Modified Campylobacter Blood-Free Selective Agar Base (CCDA)    
M98 Modified Cellobiose-Polymyxin B-Colistin (mCPC) Agar    
M103a Modified Oxford Listeria Selective Agar    
M99 Motility-Indole-Ornithine (MIO) Medium Q20 C6330, C6331, C6332, C6333, C6334
M100 Motility Medium (for B. cereus)    
M101 Motility Nitrate Medium (for Cosmetics) Y23  
M102 Motility-Nitrate Medium, Buffered
(for C. perfringens)
   
M103 Motility Test Medium (Semisolid) Q10, Q11  
M104 MR-VP Broth K37  
M105 Mucate Broth    
M106 Mucate Control Broth    
M107 Mueller Hinton Agar G45, H11 C6420, C6421, C6422, C6423, C6424
M108 Nitrate Broth K42 C6450, C6451, C6452, C6453, C6454
M109 Nitrate Broth, Enriched (CDC)    
M110 Nitrate Reduction Medium and Reagents    
M111 Nonfat Dry Milk (Reconstituted)    
M112 Nutrient Agar L20 C6460, C6461, C6462, C6463, C6464
M113 Nutrient Agar (for B. cereus) L20  
M114 Nutrient Broth K43, K243, U149 C6470, C6471, C6472, C6473, C6474
M115 Nutrient Gelatin (CDC)
(for Gram-negative nonfermentative bacteria)
Q23 C6480, C6481, C6482, C6483, C6484
M116 OF Glucose Medium, Semisolid Y57  
M118 Oxford Medium    
M117 Oxidative-Fermentative (OF) Test Medium Y51  
M118a PALCAM Listeria Selective Agar G149  
M56a Papain Solution, 5%    
M119 Penicillin-Streptomycin Solution    

FDA No.

Medium Name

Prepared Media Catalog No.

CRITERION™ Media Catalog No.
M120 Peptone Sorbitol Bile Broth    
M121 Phenol Red Carbohydrate Broth Y300 C6590, C6591, C6592, C6593, C6594
M122 Phenol Red Glucose Broth Y304 C8050, C8051, C8052, C8053, C8054
M123 Phenylalanine Deaminase Agar L21 C6600, C6601, C6602, C6603, C6604
M124 Plate Count Agar (Standard Methods) G13, G43, H53, P56, U95 C6980, C6981, C6982, C6983, C6984
M125 PMP Broth    
M126 Potassium Cyanide (KCN) Broth    
M127 Potato Dextrose Agar H51, L90, Q29, U294, U394, W60 C6620, C6621, C6622, C6623, C6624
M128 Pseudomonas Agar F
(for fluorescein production)
G198  
M129 Pseudomonas Agar P
(for pyocyanine production)
G201  
M130 Purple Carbohydrate Broth Y100-Y117 C6710, C6711, C6712, C6713, C6714
M130a Purple Carbohydrate Fermentation Broth Base    
M131 Pyrazinamidase Agar* K158  
M131a Rapid L. mono Medium    
M132 Rappaport-Vassiliadis Medium K167 C6740, C6741, C6742, C6743, C6744
M133 Sabouraud's Dextrose Agar P36, W70, X40 C6810, C6811, C6812, C6813, C6814
M133 Sabouraud's Dextrose Broth K54, U73, U74 C7560, C7561, C7562, C7563, C7564
M134 Selenite Cystine Broth K69 C6920, C6921, C6922, C6923, C6924
M30d Semisolid Medium, Modified,
for Biochemical Identification
   
M30c Semisolid Medium, Modified,
for Culture Storage
   
M135 Sheep Blood Agar A10, A127, H28, L12, P33  
M136 Shigella Broth    
M137 SIM Motility Medium Q30 C6940, C6941, C6942, C6943, C6944
M138 Simmons Citrate Agar L80 C6950, C6951, C6952, C6953, C6954
M139 Sorbitol-MacConkey Agar G36 C6170, C6171, C6172, C6173, C6174

FDA No.

Medium Name

Prepared Media Catalog No.

CRITERION™ Media Catalog No.
M140 Sporulation Broth (for C. perfringens)    
M141 Spray's Fermentation Medium
(for C. perfringens)
   
M142 Staphylococcus Agar No. 110   C6990, C6991, C6992, C6993, C6994
M143 Starch Agar   C7010, C7011, C7012, C7013, C7014
M144 T1N1 Medium    
M145 Tetrathionate Broth K65 C7060, C7061, C7062, C7063, C7064
M146 Thioglycollate Medium (Fluid) (FTG) K21  
M147 Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar G55 C7040, C7041, C7042, C7043, C7044
M148 Toluidine Blue-DNA Agar G24 C5670, C5671, C5672, C5673, C5674
M149 Triple Sugar Iron Agar (TSI) L50, R32 C7110, C7111, C7112, C7113, C7114
M150 Tryptic Novobiocin (TN) Broth    
M151 Tryptic-Peptone-Glucose-Yeast Extract Broth (TPGY)    
M151a Tryptic-Peptone-Glucose-Yeast Extract Broth with Trypsin (TPGYT)    
M152 Tryptic Soy Agar G60, G151, H19, L60, Q58, U49, U60 U158, U260, U360, U361 C7120, C7121, C7122, C7123, C7124
M152a Tryptic Soy Agar-Magnesium Sulfate-NaCl (TSAMS)    
M153 Tryptic Soy Agar with 0.6% Yeast Extract (TSAYE)    
M154 Tryptic Soy Broth K82, K83, K88, K89, K131, K182, R30, R31, R36, R41, U38, U42, U44, U46, U52, U62, U65, U67, U71, U79, U80, U133, U141, U171, U223, U242, U271, U274 C7140, C7141, C7142, C7143, C7144
M156 Tryptic Soy Broth, Modified (mTSB)    
M186 Tryptic Soy Broth (TSB) with Ferrous Sulfate    
M155 Tryptic Soy Broth (TSB) with Glycerol U127  
M154a Tryptic Soy Broth with 10% NaCl and 1% Sodium Pyruvate    
M157 Tryptic Soy Broth with 0.6% Yeast Extract (TSBYE)    
FDA No. Medium Name Prepared Media Catalog No. CRITERION™ Media Catalog No.
M158 Tryptic Soy-Polymyxin Broth    
M159 Tryptic Soy-Sheep Blood Agar A10, L12, P33  
M160 Tryptic Soy-Tryptose Broth    
M164 Tryptone Broth, 1%    
M161 Tryptone Broth and Tryptone Salt Broths    
M162 Tryptone Phosphate (TP) Broth    
M163 Tryptone Salt (T1N1) Agar    
M163 T1N2 Agar    
M165 Tryptone Yeast Extract Agar    
M166 Tryptose Blood Agar Base    
M167 Tryptose Agar (for serology)   C7170, C7171, C7172, C7173, C7174
M167 Tryptose Broth (for serology)   C7200, C7201, C7202, C7203, C7204
M168 Tryptose Phosphate Broth (TPB)   C7210, C7211, C7212, C7213, C7214
M169 Tryptose-Sulfite-Cycloserine (TSC) Agar    
M170 Tyrosine Agar    
M188 Universal Preenrichment Broth    
M171 Urea Broth    
M172 Urea Broth (Rapid) Y141  
M173 Veal Infusion Agar    
M173 Veal Infusion Broth    
M191 Vibrio parahaemolyticus Sucrose Agar (VPSA)    
M190 Vibrio vulnificus Agar (VVA)    
M174 Violet Red Bile Agar (VRBA) G78 C7260, C7261, C7262, C7263, C7264
M175 Violet Red Bile-MUG Agar (VRBA-MUG)   C7250, C7251, C7252, C7253, C7254
M176 Vogel-Johnson (VJ) Agar G193 C7280, C7281, C7282, C7283, C7284
M177 Voges-Proskauer Medium (Modified) K37  
M178 Wagatsuma Agar    
M179 Xylose Lysine Deoxycholate (XLD) Agar G65 C7320, C7321, C7322, C7323, C7324
M180 Y-1 Adrenal Cell Growth Medium    
M181 Yeast Extract (YE) Agar   C7340, C7341, C7342, C7343, C7344
FDA No. Medium Name Prepared Media Catalog No. CRITERION™ Media Catalog No.
M35 Yersinia Selective Agar (YSA)    

* Supplemental products may be required. See reference listed below.

REFERENCES

1. Vanderzant, C. and Splittstoesser, D.F., eds. Compendium of Methods for the Microbiological Examination of Foods, 8th ed., Rev. A. American Public Health Association, Washington D.C., 2005.
www.cfsan.fda.gov/~ebam/bam-mi.html.


BCM and Biosynth are registered trademarks of Biosynth AG Corporation, Switzerland.
Iragasan is a registered trademark of Geigy Chemcial Corp.
CHROMagar is a trademark of Dr. Alain Rambach, France.

092214vr