|Gram Stain:||Negative to variable. Not acid-fast.|
|Morphology:||Usually pleomorphic rods.|
|Size:||0.5 micrometers in diameter by 1.5-2.5 micrometers in length.|
|Other:||The cell walls are laminated.|
Small (1mm), beta-hemolytic colonies on media containing human blood. Colonies appear either opaque, dull white, convex, domed, or somewhat conical in shape. G. vaginalis is non-hemolytic on media containing sheep blood.
KEY BIOCHEMICAL REACTIONS
- Most stains are fastidious in growth requirements.
- Hippurate hydrolyzed.
- Diffuse beta-hemolysis on media containing human blood.
- Sodium-polyanetholsulfate (SPS)-sensitive.
Both obligately aerobic, and facultatively anaerobic; Chemoorganotrophic, having fermentative type of metabolism. Produces acid but not gas from glucose and other carbohydrates including maltose and starch.
G. vaginalis is isolated form the human genital/urinary tract and appears to have a worldwide distribution.
G. vaginalis is believed to be a contributing cause of bacterial "non-specific" vaginitis. It has also occasionally been reported to cause bacteremia in postpartum women and in patients following septic abortion and transurethral resection of the prostate.
|For culture:||Chocolate Agar, Columbia Blood Agar, V Agar.|
|For selective isolation:||V Agar with CNA.|
Brucella Broth with 20% Glycerol or Skim Milk for long-term storage at
-70 degrees C.
|Temperature:||35-37 degrees C.|
|Atmosphere:||Aerobic with increased CO 2 (5-10%).|
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