INHIBITORY MOLD AGAR

Cat. no. W25 Inhibitory Mold Agar, 15x100mm Plate, 26ml 10 plates/bag
Cat. no. W58 Inhibitory Mold Agar, 25x100mm Plate, 60ml 5 plates/bag
Cat. no. X20 Inhibitory Mold Agar, 50ml HardyFlask™, 12ml 20 flasks/box
Cat. no. L47 Inhibitory Mold Agar, 20x125mm Tube, 10ml 20 tubes/box
Cat. no. W27 Inhibitory Mold Agar with Gentamicin, 15x100mm Plate, 26ml 10 plates/bag

INTENDED USE

Hardy Diagnostics Inhibitory Mold Agar and Inhibitory Mold Agar with Gentamicin are recommended for the selective isolation of fungi.

This product is not intended to be used for the diagnosis of human disease.

SUMMARY

Inhibitory Mold Agar was formulated by Ulrich for use as a general cultivation medium for various strains of pathogenic fungi.(8)

The medium is composed of nutritional factors and inorganic salts which support the growth of most pathogenic fungi. Casein and animal tissue provide growth nutrients. Yeast extract provides a rich source of vitamins. Dextrose, starch and dextrin serve as energy sources. Essential minerals and ions are supplied by sodium chloride and metallic salts. Gram-positive and gram-negative bacteria are inhibited by chloramphenicol, a broad spectrum antimicrobic. Gram-negative microorganisms are inhibited by gentamicin.

FORMULA

Ingredients per liter of deionized water:*

Yeast Extract 5.0gm
Dextrose 5.0gm
Pancreatic Digest of Casein 3.0gm
Peptic Digest of Animal Tissue 2.0gm
Starch 2.0gm
Sodium Phosphate 2.0gm
Dextrin 1.0gm
Magnesium Sulfate 0.8gm
Manganese Sulfate 0.16gm
Chloramphenicol 0.125gm
Iron Sulfate 0.04gm
Sodium Chloride 0.04gm
Agar 15.0gm

Additionally, Inhibitory Mold Agar with Gentamicin contains:

Gentamicin Sulfate 50.0mg

Final pH 6.7 +/- 0.3 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store plates at 2-8ºC. Store tubed and bottled media at 2-30 degrees C. Store products away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), contamination, or if the expiration date has passed.

PRECAUTIONS

PROCEDURE

Specimen Collection: Infectious material should be submitted directly to the laboratory without delay and protected from excessive heat and cold. If there is to be a delay in processing, the specimen should be inoculated onto an appropriate transport media and refrigerated until inoculation. Consult listed references for information regarding the processing and inoculation of specimens.(2-6,9)

Method of Use: A non-selective medium should be inoculated in addition to the selective medium in order to ensure recovery of pathogenic fungi from potentially contaminated specimens. Inoculated media should be incubated in increased humidity at 25-30ºC. Inoculate two sets of media for isolation of fungi that cause systemic mycoses; incubate one set at 25-30ºC. and the other set at 35 +/- 2.0ºC. Examine cultures weekly for a period of four to six weeks.

INTERPRETATION OF RESULTS

Media should be examined for charateristic colonial growth and morphology. Consult listed references for the interpretation of fungal growth on this medium.(2-6,9)

LIMITATIONS

For proper identification of fungi, microscopic examination and evaluation of morphological structures is required. Further biochemical, physiological, serological tests and microscopic morphology of pure cultures are recommended for complete identification. For more information see appropriate references.

Specific strains of fungi for which the medium is designed to isolate often may be inhibited. Fungi for which the medium is designed to inhibit may grow.

A non-selective and selective medium should be inoculated for isolation of fungi from potentially contaminated specimens.

Due to the incorporation of chloramphenicol, the medium is not recommended for use in culturing sterile body fluids.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Trichophyton mentagrophytes
ATCC® 9533**
G 7 days 15-35°C Aerobic Growth
Candida albicans
ATCC® 10231**
A 48hr 35°C Aerobic Growth
Escherichia coli
ATCC® 25922**
B 24hr 35°C Aerobic Partial to complete inhibition

** Recommended QC strains for User Quality Control according to the CLSI document M22 when applicable.

User Quality Control

PHYSICAL APPEARANCE

Inhibitory Mold Agar should appear opalescent, and light amber in color; small number of fine black flecks may be present.

T. mentagrophytes growing on Inhibitory Mold Agar

Trichophyton mentagrophytes (ATCC® 9533) growing on Inhibitory Mold Agar (Cat. no. W25). Incubated aerobically for 7 days at 30ºC.

C. albicans growing on Inhibitory Mold Agar

Candida albicans (ATCC® 10231) growing on Inhibitory Mold Agar (Cat. no. W25). Incubated aerobically for 48 hours at 35ºC.



E. coli inhibited on Inhibitory Mold Agar

Escherichia coli(ATCC® 25922) growth inhibited on Inhibitory Mold Agar (Cat. no. W25). Incubated aerobically for 24 hours at 35ºC.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Versalovic, J., et al. Manual of Clinical Microbiology. American Society for Microbiology, Washington, D.C.

3. Tille, P.M., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4.Cumitech 11: Practical Methods for Culture and Identification of Fungi in the Clinical Microbiology Laboratory . 1980. American Society for Microbiology, Washington, D.C.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

6. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology. J.B. Lippincott Company, Philadelphia, PA.

7. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

8. Ulrich. 1956. Bacteriol. Proc., S.A.B.; M75, p. 87.

9. St. Germain, Guy, et al. 1996. Identifying Filamentous Fungi. Star Publishing Company, Belmont, CA.


ATCC is a registered trademark of the American Type Culture Collection.

101016vr