JORDAN'S TARTRATE AGAR

Cat. no. K26 Jordan's Tartrate Agar, 15x103mm Tube, 6ml Deep 20 tubes/box

INTENDED USE

Hardy Diagnostics Jordan's Tartrate Agar is recommended for the differentiation of Enterobacteriaceae based on tartrate utilization.

SUMMARY

Brown, Duncan and Henry reported in 1924 that the members of the paratyphoid Salmonella group had varying ability in utilizing sodium tartrate.(1) Using this information, they formulated a medium to subdivide the group based on tartrate utilization. A modification was made to this tartrate medium in 1928 in order to differentiate what was then called Salmonella typhi and Salmonella schottmuelleri.(2) Edwards and Ewing reported that Jordan's Tartrate Agar was useful in differentiating monophasic and diphasic Arizona (now called Salmonella arizonae) cultures.(3) This medium has also proved useful in differentiating Vibrio parahaemolyticus from Aeromonas species and Salmonella paratyphi from other Salmonella species.(3,4)

The casein digest provides essential growth nutrients and the sodium chloride maintains osmotic equilibrium. Sodium tartrate is the differential ingredient. If sodium tartrate is is fermented, acid is produced causing the phenol red indicator to change from red to yellow in the lower portion of the tube.

FORMULA

Ingredients per liter of deionized water:*

Pancreatic Digest of Casein 10.0gm
Sodium Potassium Tartrate 10.0gm
Sodium Chloride 5.0gm
Phenol Red 0.024gm
Agar 15.0gm

Final pH 7.7 +/- 0.3 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat and freezing.

PRECAUTIONS

PROCEDURE

1. Using a sterile needle, pick the center of a well isolated colony from a pure, 18-24 hour culture. Do not take from a liquid or broth suspension.

2. Lightly inoculate the column of medium by stabbing deeply to about one-fourth inch from the bottom of the tube.

3. Incubate tubes at 35-37ºC. for up to 72 hours.(5)

INTERPRETATION OF RESULTS

A positive test is determined by a yellow color change in the lower portion of the tube; the surface zone remains red.

A negative test shows no color change; the medium remains red throughout the tube.

LIMITATIONS

A positive test may be deoxidized from air contact. Therefore, surface results should be interpreted within 24 to 72 hours.(5)

Tartrate-positive organisms that are older than 24 hours may show weak positive reactions.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Salmonella enterica
ATCC ® 14028
E 24-48hr 35°C Aerobic Positive; yellow color change in bottom of tube
Salmonella enteritidis
ATCC ® 13076
E 24-48hr 35°C Aerobic Positive; yellow color change in bottom of tube
Salmonella paratyphi
ATCC ® 9150
E 24-48hr 35°C Aerobic Negative; no color change

User Quality Control

PHYSICAL APPEARANCE

Jordan's Tartrate Agar should appear slightly opalescent, and pink in color.

S. typhimurium growing in Jordan's Tartrate Agar

Salmonella enterica (ATCC® 14028) growing in Jordan's Tartrate Agar (Cat. no. K26). Incubated aerobically for 24 hours at 35ºC.

S. paratyphi growing in Jordan's Tartrate Agar

Salmonella paratyphi (ATCC® 9150) growing in Jordan's Tartrate Agar (Cat. no. K26). Incubated aerobically for 24 hours at 35ºC.



S. typhimurium growing in Jordan's Tartrate Agar

Uninoculated tube of Jordan's Tartrate Agar (Cat. no. K26).



REFERENCES

1. Brown, H.C., J.T. Duncan and T.A. Henry. 1924. J. Hyg.; 23:1.

2. Jordan, E.O. and P.H. Harmon. 1928. J. Infect. Dis.; 42:238-241.

3. Edwards, P.R. and W.H. Ewing. 1972. Identification of Enterobacteriaceae, 3rd ed. Burgess Publ. Co., Minneapolis, MN.

4. Sakazaki, R. 1962. Jpn. J. Med. Sci. Biol.; 14:223.

5. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

6. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

7. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

ATCC is a registered trademark of the American Type Culture Collection.

012617gr