LISTERIA ENRICHMENT BROTH

Cat. no. U93 Listeria Enrichment Broth, 4oz. Glass Bottle, 99ml 20 bottles/box

INTENDED USE

Hardy Diagnostics Listeria Enrichment Broth is used for the selective enrichment of Listeria monocytogenes.

This product is not intended to be used for the diagnosis of human disease.

SUMMARY

Listeria Enrichment Broth is used in procedures for the isolation of Listeria monocytogenes from clinical and environmental samples.(1,8) Isolation procedures for Listeria monocytogenes typically involve one or more enrichment steps prior to plating on differential media.

Listeria Enrichment Broth contains peptones and dextrose as a source of growth factors. Phosphate salts maintain the proper pH of the media. The selective agents, nalidixic acid, and acriflavine act to inhibit gram-negative and gram-positive organisms, respectively. Cycloheximide is incorporated to inhibit saprophytic fungi.

FORMULA

Ingredients per liter of deionized water:*

Pancreatic Digest of Casein 17.0gm
Yeast Extract 6.0gm
Sodium Chloride 5.0gm
Papaic Digest of Soybean Meal 3.0gm
Dextrose 2.5gm
Dipotassium Phosphate 2.5gm
Acriflavine 15.0mg
Nalidixic Acid 40.0mg
Cycloheximide 50.0mg

Final pH 7.3 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC away from direct light. Media should not be used if there are any signs of deterioration, discoloration, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

PROCEDURE

Sample Collection: Consult listed references for information on sample collection.(1,4,6-8)

Method of Use: Allow medium to warm to room temperature prior to inoculation. Consult listed references for information concerning inoculation procedures.(1,4,6-8)

INTERPRETATION OF RESULTS

Consult listed references for significance and interpretation of growth from Listeria Enrichment Broth.(1,4,6-8)

LIMITATIONS

Listeria Enrichment Broth is intended to aid in the isolation and identification of Listeria monocytogenes.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Listeria monocytogenes
ATCC® 7644
A 24-48hr 35°C Aerobic Growth
Escherichia coli
ATCC® 25922
B 24-48hr 35°C Aerobic Partial to complete inhibition
Staphylococcus aureus
ATCC® 25923
B 24-48hr 35°C Aerobic Partial to complete inhibition

User Quality Control

PHYSICAL APPEARANCE

Listeria Enrichment Broth should appear clear, and bright yellow in color.

REFERENCES

1. Jorgensen, et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

4. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

5. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

6. U.S. Food and Drug Administration. Bacteriological Analytical Manual, AOAC, Arlington, VA.
http://www.fda.gov/Food/FoodScience Research/LaboratoryMethods/ucm2006949.htm.

7. Marshall, R.T., ed. 1992. Standard Methods for the Examination of Dairy Products, 16th ed. APHA, Washington, D.C.

8. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.

9. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.


ATCC is a registered trademark of the American Type Culture Collection.

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