MARTIN LEWIS WITH LINCOMYCIN

Cat. no. E39 Martin Lewis with Lincomycin, 15x100mm Plate, 19ml 10 plates/bag
Cat. no. J44 Chocolate / Martin Lewis with Lincomycin, 15x100mm Biplate,
10ml/10ml
10 plates/bag

INTENDED USE

Hardy Diagnostics Martin Lewis with Lincomycin is a selective medium for the recovery of Neisseria gonorrhoeae from both genital and oropharyngeal specimens.

SUMMARY

Several successive media have been developed for the isolation of pathogenic Neisseria from specimens containing mixed flora. Each successive formula, Thayer Martin Selective Agar, Modified Thayer Martin and Martin Lewis Agar, provides greater inhibition of contaminating flora, however, each has been shown to be inhibitory to certain vancomycin sensitive strains of Neisseria spp. Martin Lewis Agar with Lincomycin provides a rich, selective growth media with decreased vancomycin.

FORMULA

Ingredients per liter of deionized water:*

Hemoglobin, Bovine 10.0gm
Pancreatic Digest of Casein 7.5gm
Peptic Digest of Animal Tissue 7.5gm
Sodium Chloride 5.0gm
Dipotassium Phosphate 4.0gm
Monopotassium Phosphate 1.0gm
Corn Starch 1.0gm
Colistin 7.5mg
Trimethoprim Lactate 5.0mg
Vancomycin 2.0mg
Amphotericin B 1.5mg
Lincomycin 1.0mg
Koenzyme Enrichments 10.0ml
Agar 10.0gm

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), hemolysis, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

PROCEDURE

Specimen Collection: Specimens should be submitted directly to the laboratory without delay and protected from excessive heat and cold. If there is to be a delay in processing, specimens should be inoculated into an appropriate transport such as Amies with charcoal. Specimens must be transported at ambient temperatures (15-30ºC). Do not refrigerate. Refer to listed references for more information on specimen collection and transport.(1-3)

Method of Use: Bring media to room temperature before use. Inoculate media and streak to obtain isolated colonies. Incubate 24-48 hours at 35ºC. in 5-10% CO2. Some strains may require up to 72 hours to appear.

INTERPRETATION OF RESULTS

Neisseria gonorrhoeae appears as small, grayish-white to colorless mucoid colonies. N. meningiditis forms similar colonies to N. gonorrhoeae , but larger and blue-gray.

An oxidase test may be performed from the primary plate for presumptive identification.

LIMITATIONS

This medium is intended for primary isolation. Some diagnostic tests may be performed with the primary plate. However, additional tests including gram stain and biochemical testing should be performed on pure cultures for complete identification. For more information, see appropriate references.

The agents in selective media may inhibit some strains of desired species or permit the growth of species they were designed to inhibit. Therefore, specimens cultured on selective media should also be cultured on non-selective media to obtain additional information and to help insure recovery of potential pathogens.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Neisseria gonorrhoeae
ATCC ® 43069
A 24-48hr 35°C CO 2 ** Growth
Neisseria meningitidis
ATCC ® 13090***
A 24-48hr 35°C CO 2 ** Growth
Proteus mirabilis
ATCC ® 43071
B 24-48hr 35°C CO 2 ** Partial to complete inhibition, no swarming
Staphylococcus epidermidis
ATCC ® 12228
B 24-48hr 35°C CO 2 ** Partial to complete inhibition
Escherichia coli
ATCC ® 25922***
B 24-48hr 35°C CO 2 ** Partial to complete inhibition
Candida albicans
ATCC ® 60193***
B 24-48hr 35°C CO 2 ** Partial to complete inhibition
Neisseria sicca
ATCC ® 9913***
B 24-48hr 35°C CO 2 ** Inhibited

USER QUALITY CONTROL

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

4. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

5. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

6. Evans, G.L., et al. 1989. New selective medium for the isolation of Neisseria gonorrhoeae. J. Clin. Microbiol.; 27:2471-2474.


ATCC is a registered trademark of the American Type Culture Collection.

032116gr