SELECTION OF PRIMARY MEDIA FOR BACTERIOLOGY SPECIMENS

SPECIMEN ROUTINE MEDIA SUGGESTED ADDITIONAL
MEDIA
Abscess/pus (closed wound):

BAP, MAC, BRU, Thio, GS As smear indicates; CNA
Autopsy, blood, tissue:




Follow procedure for "living" material
Blood:
Peripheral blood Choc, BRU As smear indicates
Bone marrow BAP, MAC, Choc

Body fluids (except blood, CSF, urine):
Centrifuge fluids for 15min at 2500rpm except when grossly purulent. Stain and culture sediment. Incubate supernatant for 24 hours at 27 degrees C. Filtration is also useful if the filter can be cultured directly.
Bile BAP, MAC, BRU, Thio, GS CNA, HE, XLD, GN
Hematoma Treat as abscess, GS CNA
Joint BAP, Choc, Thio, GS

Pericardial, peritoneal BAP, MAC, BRU, Choc, Thio, GS CNA
Pleural empyema BAP, MAC, BRU, Choc, Thio, GS CNA
Catheters:
Foley Do not culture
Central venous pressure lines, umbilical or intravenous catheters BAP Thio
Central nervous system:
Brain tissue Treat as abscess, GS
CSF, shunt, meningomyelocele:


BAP, Choc, Thio, GS Centrifuge for
15 min. at 2,500rpm
As smear indicates; MAC, CNA, BRU
SPECIMEN ROUTINE MEDIA SUGGESTED ADDITIONAL
MEDIA
Ear:
Internal BAP, MAC, Choc, Thio, GS CNA
External BAP, MAC, Choc, GS CNA
Eye:
Conjunctiva BAP, MAC, Choc, Thio, GS TM, CNA
Other BAP, MAC, Choc, BRU, Thio, GS CNA
Genital tract - female:
Amniotic fluid BAP, MAC, TM, Choc, BRU, Thio, GS CNA
Cervix TM, GS Choc
Cul-de-sac BAP, MAC, Choc, TM, BRU, Thio, GS CNA, V Agar
Uterine material
(endometrium, products of conception;
fetus, placenta, lochia),
tubes, ovaries


BAP, MAC, Choc, TM, BRU, Thio, GS CNA, V Agar, BRU
Intrauterine device Thio

Urethra TM, GS Choc
Vagina (except cuff) TM, GS Choc, BAP
Vaginal cuff See Abscess/pus

Vulva (Bartholin abscess) BAP, MAC, TM, BRU, Thio, GS CNA, V Agar
Genital tract - male:
Prostate fluid BAP, MAC, TM, Thio, GS CNA
Testes and epididymis (aspirate) BAP, CNA, TM, MAC, GS

Urethra TM, GS

Intestinal tract:
Colostomy, Ileostomy, feces,
Rectal Swab
BAP, MAC, HE, XLD, GN, Campy
(42 degrees C.), Stain with Methylene Blue for presence of WBC only
CIN, MAC with Sorbitol
Gastric aspirate BAP, MAC, Choc, GS CNA
Plastic prostheses:


Thio

Pus - closed wound:


See Abscess/pus

Respiratory tract:
Throat/pharynx Selective Strep Agar

Epiglottis BAP, Choc CNA
Nasal sinus BAP, MAC, Choc, BRU, Thio, GS CNA
Nasopharynx BAP, Choc CNA
Nose BAP, CNA Mannitol Salt
Pleural fluid See Body fluids

Sputum BAP, MAC, Choc, GS CNA, Mycobacteria Setup
Bronchial secretions BAP, MAC, Choc, GS CNA, Mycobacteria Setup
Transtracheal aspirate BAP, MAC, Choc, BRU, Thio, GS CNA, Mycobacteria Setup
Tracheal aspirate BAP, MAC, Choc, GS CNA
SPECIMEN ROUTINE MEDIA SUGGESTED ADDITIONAL
MEDIA
Respiratory tract:
Oral cavity GS

Dental abscess BAP, MAC, BRU, Thio, GS As smear indicates, CNA
Skin:
Deep wound (open) BAP, MAC, GS CNA, Choc
Superficial wound:

BAP, MAC, GS
Traumatized areas
(burns, bites, decubitus ulcers)
BAP, MAC, Thio As smear indicates; BRU, CNA
Rash, non-purulent lesions BAP, MAC, Choc, Thio, GS CNA
Tissue:
Grind with sterile Alundum (aluminum oxide) with a sterile mortar and pestle or sterile tissue grinder or homogenize. Use enough broth to give a 10%-20% suspension.
Surgical/biopsy BAP, MAC, BRU, Thio, GS CNA
Urine, Clean-voided, catheterized or ileal loop urine:

BAP, MAC, GS CNA
Suprapubic bladder tap; cystoscopy or ureterostomy urine:

BAP, MAC, BRU, Thio
(0.1ml per plate)
CNA


MEDIA PRODUCT NAME CATALOG NUMBER
BAP Blood Agar, 5% Sheep Blood A10
BRU Brucella with Hemin and Vitamin K A30
Choc Chocolate Agar E14
CNA Columbia CNA (with Colistin and Nalidixic Acid) A50
GN GN (Gram-Negative) Broth K39
GS Gram Stain GK400
HE Hektoen Enteric Agar G63
MAC MacConkey Agar G35
Thio Thioglycollate with Supplements K23
TM Thayer Martin, Modified E30
XLD Xylose Lysine Deoxycholate G65

REFERENCES

1. Forbes, B.A., et al. 1998. Bailey and Scott's Diagnostic Microbiology , 10th ed. C.V. Mosby Company, St. Louis, MO.


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