MYCOSEAL™

Cat. no. SS9225 MycoSeal™, 500ml jar 60 shrink seals/jar
Cat. no. SS6000 MycoSeal™, 5 gallon bucket 6000 shrink seals/bucket

INTENDED USE

Hardy Diagnostics MycoSeal™ cellulose shrink seals are recommended for sealing 15x100mm petri plates.

SUMMARY

MycoSeal™ is a cellulose shrink seal designed to keep petri plate lids tightly sealed. Sealed plates retain their moisture and stay fresh longer. Ideal for mycology and TB cultures, MycoSeal™ prevents dehydration and cracking of the media and yet still allows both oxygen and carbon dioxide gas exchange.(3) The tight seal also prevents the accidental release of mycobacteria and fungal spores.

MycoSeals™ are also recommended for use with the "micro colony" method for early detection of mycobacteria growing on a Middlebrook 7H11 Agar plate.(4)

FORMULA

Each container of MycoSeal™ contains cellulose shrink seals (60/jar or 6000/bucket) in a preservative solution. The solution contains 7% polyethylene glycol, 0.1% para chloro meta cresol, and 0.02% chlorhexidene diacetate, in deionized water.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-30ºC. Protect from excessive heat.

PRECAUTIONS

PROCEDURE

After inoculating, place petri dish on a flat surface, with lid facing down. Then place seal around the plate and leave undisturbed. The seal will shrink and seal around the plate in about 2 hours at room temperature (2-30ºC.). Sealing time is shorter if placed in the incubator. The seal can easily be cut for removal.

Due to the volume of liquid in the jar used to preserve the seals, place the jar on a secure, solid surface before closing. Press evenly on the lid until it clicks to align the threads. Once the threads are aligned, the lid can be tightened securely for long-term storage.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as inoculating loops, needles, incubators, incinerators, biological safety cabinet, petri plates, and microbiological culture media, etc., are not provided.

PHYSICAL APPEARANCE

MycoSeal™ should appear as clear celon bands in a clear, colorless liquid.

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Traylor, M.T. 1994. CO2 penetration of MycoSeal™ cellulose shrink seals: an in-house study. Hardy Diagnostics.

4. Welch, David F., et al. 1993. Journal of Clinical Microbiology, Vol. 31, No. 8; 2178-2184. American Society for Microbiology.


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