Cat. no. K43 Nutrient Broth, 15x103mm Tube, 5ml 20 tubes/box
Cat. no. K243 Nutrient Broth, 20x150mm Tube, 20ml 20 tubes/box
Cat. no. U234 Nutrient Broth, 125ml Polypropylene Bottle, 100ml 12 bottles/box


Hardy Diagnostics Nutrient Broth is a general purpose growth medium recommended for use in the cultivation of nonfastidious microorganisms.

Cat. no. U234 is not intended to be used for the diagnosis of human disease.


Nutrient Broth can be used as a pre-enrichment medium and may also be used in accordance with many standard methods procedures for testing food, milk and dairy products.

Hardy Diagnostics Nutrient Broth is a liquid medium that contains peptone and beef extract. Nutrients necessary for the replication and growth of a large number of nonfastidious microorganisms are provided by this simple formulation. Water soluble substances including carbohydrates, vitamins, organic nitrogen compounds and salts are present in beef extract.(7) Pancreatic digest of gelatin supplies the principle source of organic nitrogen in the form of amino acids and long-chain fatty acids.


Ingredients per liter of deionized water:*

Pancreatic Digest of Gelatin 5.0gm
Beef Extract 3.0gm

Final pH 6.8 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.


Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration, discoloration, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.


For Cat. nos. K43 and K243.

For Cat. no. U234.


Specimen Collection: Consult listed references for information on specimen collection.(1,4,6,9) Infectious material should be submitted directly to the laboratory without delay and protected from excessive heat and cold. If there is to be a delay in processing, the specimen should be inoculated onto an appropriate transport media and refrigerated until inoculation.


Consult listed references for the isolation of microorganisms and further biochemical tests required for identification.(1-4,6,9)



Standard microbiological supplies and equipment such as loops, swabs, applicator sticks, other culture media, supplemental ingredients, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.


Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Escherichia coli
ATCC® 25922
A 24-48hr 35°C Aerobic Growth; turbidity
Pseudomonas aeruginosa
ATCC® 27853
A 24-48hr 35°C Aerobic Growth; turbidity
Staphylococcus aureus
ATCC® 25923
A 24-48hr 35°C Aerobic Growth; turbidity


Physical Appearance

Hardy Diagnostics Nutrient Broth should appear clear, and light amber in color.


1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Greenberg, A.E., et al., (ed.). 2005. Standard Methods for the Examination of Water and Wastewater, 21th ed., APHA, Washington, D.C.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

5. MacFaddin, J.F. 1985. Media for Isolation, Cultivation, Identification, Maintenance of Bacteria, Vol. I. Williams & Wilkins, Baltimore, MD.

6. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

7. Pelczar, Chan and Kreig. 1986. Microbiology, 5th ed. McGraw-Hill Book Company, New York.

8. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

9. Vanderzant, C. and D.F. Splittstoesser, (ed.). 1992. Compendium of Methods for the Microbiological Examination of Foods, 3rd ed. APHA, Washington, D.C.

ATCC is a registered trademark of the American Type Culture Collection.