OxiStrips™ Oxidase Strips and OxiSticks™ Oxidase Swabs

Cat. no. Z93 OxiStrips™ Oxidase Strips 25 strips/vial
Cat. no. Z193 OxiSticks™ Oxidase Swabs 25 swabs/vial

INTENDED USE

Hardy Diagnostics OxiStrips™ Oxidase Strips and OxiSticks™ Oxidase Swabs are used for the detection of cytochrome oxidase activity in bacteria.

SUMMARY

Cytochrome containing organisms produce an intracellular oxidase enzyme. This oxidase enzyme catalyzes the oxidation of cytochrome c. Organisms which contain cytochrome c as part of their respiratory chain are oxidase-positive and turn the reagent blue/purple. Organisms lacking cytochrome c as part of their respiratory chain do not oxidize the reagent, leaving it colorless within the limits of the test, and are oxidase-negative.

OxiStrips™ Oxidase Strips are ready to use test strips with a convenient plastic handle so the user can avoid skin contact with the reaction area.

OxiSticks™ Oxidase Swabs are swabs containing reagents impregnated in the tip of the swab for ease of use.

REAGENT FORMULA

OxiStrips™ Oxidase Strips and OxiSticks™ Oxidase Swabs are impregnated with N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride, in a preservative solution.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 15-30ºC. Products should not be used if there are any signs of deterioration or if the expiration date has passed. Store OxiStrips™ and OxiSticks™ with a desiccant in the vial at all times.

PRECAUTIONS

PROCEDURE

Specimen Collection: This product is not intended for primary isolation of patient specimens. This product is used in conjunction with other biochemical tests to identify cultures of isolated organisms.

Method of Use:

OxiStrips™ Oxidase Strips: Place oxidase test strip in a petri dish and moisten an area of the strip to be tested with water. Do not saturate strip. With either a platinum loop or wooden applicator stick, smear a bacterial paste from 3-4 well isolated colonies onto the moistened area. Use colonies that are 18-24 hours old.

OxiSticks™ Oxidase Swabs: Remove swab from container without touching the tip. Use the swab to carefully collect 3-4 well isolated colonies. There is no need to pre-moisten the swab. Use colonies that are 18-24 hours old.

INTERPRETATION OF RESULTS

Appearance of a blue/purple color within 30 seconds indicates a positive test.

Important: Any color appearing after this time should be disregarded.

LIMITATIONS

The oxidase test may be used in the presumptive identification of Neisseria spp. and in the differentiation and identification of gram-negative bacilli. All oxidase-positive organisms should be examined by gram stain to determine cellular morphology and gram reaction. It is recommended that biochemical, immunological, molecular, or mass spectrometry testing be performed on colonies from pure culture for complete identification.

Using nichrome or other iron containing inoculating devices may cause false-positive reactions.

Oxidase reactions of gram-negative bacilli should be determined on colonies obtained from non-selective and non-differential media to ensure valid results.

Most Haemophilus spp. are oxidase-positive. Less sensitive oxidase test strips or reagents may yield false-negative results. Consult listed reference for more information.(7)

Weakly oxidase-positive organisms, such as Pasteurella multocida, may take longer to show a positive reaction on the test strips.

It is recommended to use colonies that are 18-24 hours old. Older colonies may produce weaker reactions.

The oxidase test should be performed on isolates at or above 15-30ºC.

Any color development appearing after 30 seconds of inoculation should be disregarded.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, pipets, incubators, and incinerators, etc., as well as biochemical and serological reagents, are not provided.

QUALITY CONTROL

Test Organisms Reaction
Pseudomonas aeruginosa
ATCC® 27853
Oxidase-positive; blue/purple color develops within 10-20 seconds
Escherichia coli
ATCC®25922
Oxidase-negative; no color develops

User Quality Control

PHYSICAL APPEARANCE

OxiStrips™ Oxidase Strips are reagent test strips with a plastic handle, and white in color. OxiSticks™ Oxidase Swabs are polypropylene shafted swabs with dacron tips that are white in color.

Positive Oxidase Test

Pseudomonas aeruginosa (ATCC® 27853) applied to an OxiStrip™ (Cat. no. Z93). The development of a blue/purple color within 10-20 seconds was indicative of a positive oxidase reaction.

Negative Oxidase Test

Escherichia coli (ATCC® 25922) applied to an OxiStrip™ (Cat. no. Z93). No development of a blue/purple color within 10-20 seconds was indicative of a negative oxidase reaction.

P. aeuriginosa OxiSticks™ Oxidase Swabs

Pseudomonas aeruginosa (ATCC® 27853) applied to an OxiStick™ (Cat. no. Z193). The development of a blue/purple color within 10-20 seconds was indicative of a positive oxidase reaction.

REFERENCES

1. Versalovic, J., et al. Manual of Clinical Microbiology. American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Commission on Laboratory Accreditation, Laboratory Accreditation Program Microbiology Checklist. College of American Pathologists. Rev. 9/30/2004.

4. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

5. MacFaddin, J.F. Biochemical Tests for Identification of Medical Bacteria,, Lipincott Williams & Wilkins, Philadelphia, PA.

6. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

7. Holt, J.G. and N.R. Krieg. 1984. Bergy's Manual of Systematic Bacteriology, Vol. I. Williams & Wilkins, Baltimore, MD.

8. Centers for Medicare and Medicaid, Appendix C, Survey Procedures and Interpretive Guidelines for Laboratories and Laboratory Services . Subpart K - Quality System for Non-Waived Testing. 493;1200-1265. www.cms.hhs.gov/clia.


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