PEPTIC DIGEST AGAR (PDA) WITH FILDES AND NAFCILLIN

Cat. no. G141 PDA with Fildes and Nafcillin, 15x100mm Plate, 19ml 10 plates/bag

INTENDED USE

Hardy Diagnostics Peptic Digest Agar (PDA) with Fildes and Nafcillin is an enrichment medium recommended for the selective isolation of Haemophilus species.

SUMMARY

Peptic Digest Agar with Fildes and Nafcillin is composed of Columbia Agar Base supplemented with Fildes enrichment and nafcillin. Fildes enrichment is a sterile digest of sheep blood which is rich in hemin (X-Factor) and nicotinamide adenine dinucleotide (V-Factor), the factors required by Haemophilus influenzae and other members of the Haemophilus group. Nafcillin inhibits most normal respiratory flora, thereby making the medium selective for Haemophilus spp.

FORMULA

Ingredients per liter of deionized water:*

Yeast Enriched Peptone 10.0gm
Peptic Digest of Animal Tissue 8.0gm
Pancreatic Digest of Casein 5.0gm
Sodium Chloride 5.0gm
Corn Starch 1.0gm
Nafcillin 2.0mg
Fildes Supplement 25.0ml
Agar 14.0gm

Final pH 7.3 +/- 0.3 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

PROCEDURE

Specimen Collection: Infectious material should be submitted directly to the laboratory without delay and protected from excessive heat and cold. If there is to be a delay in processing, the specimen should be inoculated onto an appropriate transport media and refrigerated until inoculation.

Method of Use: Prior to inoculation, the medium should be brought to room temperature. Inoculate the specimen onto the medium and streak for isolation using the four quadrant technique. Incubate the plate in 5-10% CO 2 at 35-37ºC. for 24-48 hours. Examine plates for growth and typical colonial morphology.

INTERPRETATION OF RESULTS

Colonies of Haemophilus spp. appear transparent, moist, convex and smooth and emit a distinctive "mousy" odor.

Gram stain and biochemical testing must be performed on isolated organism(s) to confirm identification.

LIMITATIONS

This product is used in conjunction with other biochemical tests to identify cultures of isolated organism.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Haemophilus influenzae
ATCC ® 10211
A 24-48hr 35°C CO 2 ** Growth
Staphylococcus aureus
ATCC ® 25923
B 24hr 35°C Aerobic Inhibited

** Atmosphere of incubation is enriched with 5-10% CO2.

USER QUALITY CONTROL

Physical Appearance

Peptic Digest Agar (PDA) with Fildes and Nafcillin should appear transparent and colorless.

H. influenzae growing on PDA with Fildes and Nafcillin

Haemophilus influenzae (ATCC ® 10211) colonies growing on PDA with Fildes and Nafcillin (Cat. no. G141). Incubated in CO 2 for 24 hours at 35ºC.

S. epidermidis inhibited on PDA with Fildes and Nafcillin

Staphylococcus epidermidis (ATCC ® 12228) growth inhibited on PDA with Fildes and Nafcillin (Cat. no. G141). Incubated aerobically for 24 hours at 35ºC.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

5. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.


ATCC is a registered trademark of the American Type Culture Collection.

040416gr