PEDIOCOCCUS

8 plus species including:

SPECIES

MICROSCOPIC APPEARANCE

Gram Stain: Gram-positive.
Morphology: Spherical, never elongated. Division occurs alternately in two pairs at right angles to form tetrads. Single cells are rare and chains are not formed.
Size: 1.0 micrometers by 2.5 micrometers.
Motility: Non-motile.
Capsules: No.
Spores: No.

MACROSCOPIC APPEARANCE

Colonies appear smooth, round, and grayish-white.

KEY BIOCHEMICAL REACTIONS

METABOLIC PROPERTIES

Facultatively anaerobic or microaerophilic. Chemoorganotrophic. Cells require nutritionally rich media and fermentable carbohydrates. Glucose is fermented with the production of acid, but not gas.

HABITAT

Beer and brewery processes, silage, pickled vegetables and dairy products.

PATHOGENICITY

Non-pathogenic to plants or animals.

RECOMMENDED MEDIA

For culture: MRS Agar or MRS Broth.
For selective isolation: Tomato Juice Agar, Universal Beer Agar (UBA), MRS Agar, or Modified Thayer Martin.
For maintenance: Skim Milk enriched with glucose, yeast, and calcium carbohydrate for short-term maintenance and lyophilization for long-term storage.

INCUBATION

Temperature: 25-40 degrees C.
Time: 24-48 hours.
Atmosphere: Anaerobic.
Optimum pH: 4.5-7.0.

REFERENCES

1. Holt, J.G., et al. 1994. Bergey's Manual of Determinative Bacteriology , 9th ed. Williams & Wilkins, Baltimore, MD.

2. Holt, J.G., et al. 1986. Bergey's Manual of Systemic Bacteriology , Vol. I & II. Williams & Wilkins, Baltimore, MD.

3. The Oxoid Vade-Mecum of Microbiology . 1993. Unipath Ltd., Basingstoke, UK.

4. Murray, P.R., et al. 1995. Manual of Clinical Microbiology , 6th ed. American Society for Microbiology, Washington, D.C.

5. Internet: www.hardlink.com /Bacterial Database Search, February, 1998.

6. Hensyl, B.R., et al. 1990. Stedman's Medical Dictionary , 25th ed. Williams & Wilkins, Baltimore, MD.

7. Koneman, et al. 1997. Color Atlas and Textbook of Diagnostic Microbiology , 5th ed. Lippincott, Philadelphia, PA.


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