Peptone Waters, 0.1%

Cat. no. K47 Peptone Water, 0.1% with 1% NaCl, 20x125mm Tube, 9ml 20 tubes/box
Cat. no. U201 Peptone Water, 0.1%, 500ml Polycarbonate Bottle, 500ml 10 bottles/box


Hardy Diagnostics Peptone Waters, 0.1% are recommended for use in food, water and wastewater, pharmaceutical and cosmetic testing as a diluent or rinsing solution and for microbial enumeration purposes.

This product is not intended to be used for the diagnosis of human disease.


Peptone Waters, 0.1% are minimal nutrient media designed to reduce multiplication of microorganisms. The media are useful as diluent or rinsing solutions, or for making suspensions of non-fastidious microorganisms for microbial enumeration procedures. (2,3,5-11) Peptone Water, 0.1% with 1% sodium chloride helps to maintain osmotic balance and preserve sublethally injured cells. Peptone Waters, 0.1% are not recommended for the growth or maintenance of fastidious microorganisms. (2,3)


Ingredients per liter of deionized water:*

Peptone 1.0gm

Final pH 7.2 +/- 0.2 at 25ºC.

In addition, Peptone Water, 0.1% with 1% NaCl contains:

Sodium Chloride 10.0gm

Final pH 8.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.


Storage: Upon receipt store at 2-30ºC. away from direct light. Media should not be used if there are any signs of deterioration, discoloration, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.



For use as a diluting or rinsing fluid or in the preparation of suspensions for enumeration procedures. See appropriate references or regulatory guidelines for best practices. (2,3,5-11)


Do not suspend bacteria in any peptone water for more than 30 minutes at room temperature because death or microbial multiplication may occur.


Standard microbiological supplies and equipment such as loops, swabs, applicator sticks, other culture media, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.


Hardy Diagnostics tests Peptone Waters, 0.1% for sterility, pH, and fill volume.


Physical Appearance

Peptone Waters, 0.1% should appear clear, and colorless in color.


1. Anderson, N.L., et al. 2005. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

4. Quality Assurance for Commercially Prepared Microbiological Culture Media , M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

5. American Public Health Association. 2012. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.

6. Association of Official Analytical Chemists. Official Methods of Analysis sm , AOAC, Washington, D.C.

7. The Official Compendia of Standards. USP General Chapter <61> Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests. USP-NF . United States Pharmacopeial Convention Inc., Rockville, MD.

8. American Public Health Association. Standard Methods for the Examination of Dairy Products, APHA, Washington, D.C.

9. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.

10. U.S. Food and Drug Administration. Hazard Analysis and Critical Control Points (HACCP) . Silver Spring, MD.

11. U.S. Food and Drug Administration. Bacteriological Analytical Manual. AOAC, Arlington, VA.