PEPTOSTREPTOCOCCUS

18 plus species including:

SPECIES

MICROSCOPIC APPEARANCE

Gram Stain: Gram-positive.
Morphology: Spherical, sometimes ovoid; arrangement is variable: in pairs, tetrads, clumps, or chains.
Size: 0.5 micrometers to 1.2 micrometers.
Motility: Non-motile.
Capsules: None.
Spores: None.

MACROSCOPIC APPEARANCE

Colonies vary from raised, dull, smooth, non-hemolytic to convex and dull. Often colonies display a slightly yellow pigment.

KEY BIOCHEMICAL REACTIONS

METABOLIC PROPERTIES

Anaerobic. Chemoorganotrophic with a fermentative type metabolism. Acid is produced from glucose and other carbohydrates. Requires nutritionally rich media.

HABITAT

Skin of umbilicus and genital area of human females. Occasionally present in clinical specimens. Obligate parasites of the mouth, mucous membranes, respiratory, and intestinal tract of mammals.

PATHOGENICITY

Species is known to play a part in purulent and pyogenic infections, putrefactive war wounds, and appendicitis. The most common clinical isolates are P . magnus , P . anaerobius , and P . asaccharolyticus . Also, P . magnus has been isolated in infections of prosthetic devices. (8)

RECOMMENDED MEDIA

For culture: Yeast Extract Agar, Brucella with H & K, Thioglycollate with supplements.
For selective isolation: Anaerobic PEA or Anaerobic CNA.
For maintenance: Cooked Meat Medium for short-term maintenance. Lyophilization is required for long-term storage.

INCUBATION

Temperature: 35-37 degrees C.
Time: 24-48 hours.
Atmosphere: Anaerobic.

REFERENCES

1. Holt, J.G., et al. 1994. Bergey's Manual of Determinative Bacteriology , 9th ed. Williams & Wilkins, Baltimore, MD.

2. Holt, J.G., et al. 1986. Bergey's Manual of Systemic Bacteriology , Vol. I & II. Williams & Wilkins, Baltimore, MD.

3. The Oxoid Vade-Mecum of Microbiology . 1993. Unipath Ltd., Basingstoke, UK.

4. Murray, P.R., et al. 1995. Manual of Clinical Microbiology , 6th ed. American Society for Microbiology, Washington, D.C.

5. Internet: www.hardlink.com /Bacterial Database Search, February, 1998.

6. Hensyl, B.R., et al. 1990. Stedman's Medical Dictionary , 25th ed. Williams & Wilkins, Baltimore, MD.

7. Koneman, et al. 1997. Color Atlas and Textbook of Diagnostic Microbiology , 5th ed. Lippincott, Philadelphia, PA.

8. Howard, B.J., et al. 1994. Clinical and Pathogenic Microbiology, 2nd ed. Mosby, St. Louis, IL.


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