PHIALOPHORA

SPECIES

MICROSCOPIC APPEARANCE

Hyphae septate, hyaline to brown. Phialides pale-brown to brown, bottle-shaped or cylindrical, with a collarette at the tip. Conidia unicellular, hyaline or brown, round, ovoid, cylindrical or curbed, typically accumulating at the tips of phialides in slimy masses.

APPEARANCE

Surface: Texture velvety to wooly; dark gray, brown or black on surface.
Reverse: Dark gray, brown or black.
Growth Rate: Slow growth.

DISTINGUISHING FEATURES

Phialophora is distinguished from Exophiala and Wangiella dermatitidis by its phialides with readily visible collarettes. The shape of the collarettes is important in identification of the medically important species; those of Phialophora verrucosa are "vase-shaped", those of P . richardsiae are "saucer-shaped" and "vase-shaped".

HABITAT

Phialophora are cosmopolitan, saprobes isolated commonly from decomposing wood, soil and subaquatic debris in bodies of cold fresh water.

PATHOGENICITY

The genus Phialophora includes P . verrucosa , one of the agents of chromoblastomycosis. It also includes several species causing diverse types of phaeohyphomycosis, presenting in the form of mycotic arthritis, subcutaneous cyst, osteomyelitis, and cerebral or disseminated infection.

RECOMMENDED MEDIA

INCUBATION

Temperature: 25 degrees C.
Time: 10-14 days.

REFERENCES

1. Hensyl, William R., et al. 1990. Stedman's Medical Dictionary , 25th ed. Williams & Wilkins, Baltimore, MD.

2. St. Germain, Guy and Summerbell, Richard, Ph.D. 1996. Identifying Filamentous Fungi , 1st ed. Star Publishing Company, Belmont, CA.

3. Larone, Davise, H. 1995. Medically Important Fungi , A Guide to Identification , 3rd ed. American Society for Microbiology Press, Washington, D.C.

4. The Oxoid Vade-Mecum of Microbiology . 1993. Unipath Ltd., Basingstoke, UK.

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