PRECAUTIONS WHEN USING MEDIA
All media products are labeled for "in vitro diagnostic use only" or "for laboratory use only." Products labeled "for laboratory use only" are not intended for human or animal therapeutic use.
Products are to be used only by adequately trained and qualified laboratory personnel. Observe approved biohazard precautions and aseptic technique. All patient and laboratory specimens should be considered infectious and handled according to "standard precautions". The "Guideline for Isolation Precautions" is available from the Centers for Disease Control and Prevention at www.cdc.gov/ncidod/dhqp/gl_isolation.html.
For additional information regarding specific precautions for the prevention of the transmission of all infectious agents from laboratory instruments and materials, and for the recommendations for the management of exposure to infectious disease, refer to the CLSI document M-29.
Sterilize all biohazardous waste before disposal.
The use of a biological safety cabinet is recommended when culturing or processing specimens for fungi, mycobacteria, or for any procedure that may create dangerous aerosols.
After use, all media, specimens, and containers must be sterilized by incineration or in an autoclave before disposal (121 degrees C. for 30 minutes is recommended as a minimum).
Care should be exercised in the opening of tubes with tight caps to prevent the breakage of the glass.
Care should be taken to avoid contact with skin, eyes, or mucous membranes when handling culture media or any laboratory reagent, stain, fixative, or chemical. If contact occurs, flush immediately with running water. Contact a physician, hospital, or poison control center if overexposure or irritation exists.
Observe all precautions on the Material Safety Data Sheets. See "Material Safety Data Sheets (MSDS)" for more information on safety.
INFORMATION FOR FLASKS
Condensation may cause fogging of the upper surface of the flask, which may obstruct your view of the culture.
Rapid cooling after incubation causes condensation of the upper surface of the flask. Condensation in the flasks can be prevented by:
1. Inverting the flask during incubation and examining the cultures immediately after removing them from the incubator before any fogging occurs.
2. Keeping an uninoculated flask on top and below your incubating cultures. These insulating "dummy flasks" help to slow down the cooling process.
3. If condensation has already formed, it can be removed by tapping the flask on the counter top or by inserting a sterile cotton swab into the flask and wiping the inner surface from side to side.
REMEMBER: For air exchange, incubate the flasks with loose caps (except Transgrow, Cat. no. X52).
The medium should not be used if it shows signs of microbial contamination, dehydration (shrinking or cracking of the medium), hemolysis, discoloration, or is beyond the expiration date. Please see "Problem Reporting".
Protection of Laboratory Workers from Occupationally Acquired Infections, Approved Guideline M29. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.