SABOURAUD DEXTROSE (SABDEX) MEDIA, IRRADIATED

Cat. no. P750 SabDex Agar with Lecithin and Tween® 80, Irradiated, 15x60mm Contact Plate, TapTight™, 15ml, Triple Bagged 10 plates/bag
Cat. no. P890 SabDex Agar with Lecithin and Tween® 80, Irradiated, 15x60mm Contact Plate, 15ml, Triple Bagged 10 plates/bag
Cat. no. W257 SabDex Agar with Lecithin and Tween® 80, Irradiated, 15x100mm plate, 34ml, Triple Bagged 10 plates/bag

INTENDED USE

Irradiated Sabouraud Dextrose (SabDex) Agar with Lecithin and Tween® 80 is recommended for the detection and enumeration of yeasts and fungi from environmental sources in a clean room setting.

This product is not intended to be used for the diagnosis of human disease.

SUMMARY

Sabouraud Dextrose Agar was formulated by Sabouraud in 1892, for culturing dermatophytes.(6) The pH is adjusted to approximately 5.6 in order to enhance the growth of fungi, especially dermatophytes, and to slightly inhibit bacterial growth. This medium is recommended for mold and yeast counts by the United States Pharmacopeia, Standard Methods for the Examination of Water and Wastewater, the Association of Official Analytical Chemists, and the Compendium of Methods for the Microbiological Examination of Foods.(1-3,7,8)

Sabouraud Dextrose (SabDex) Agar with Lecithin and Tween® 80 contains digests of animal tissues (peptones), which provide a nutritious source of amino acids and nitrogenous compounds for the growth of fungi and yeasts. Dextrose is added as the energy and carbon source. Lecithin and Tween® 80 serve to neutralize quarternary ammonium compounds and phenolic disinfectants, respectively.

Sabouraud Dextrose (SabDex) Agar with Lecithin and Tween® 80 Contact Plates are used to sample environmental surfaces for the presence of yeasts and fungi, using the Contact Method for sampling floors, counters, etc.

FORMULA

Ingredients per liter of deionized water:*

SabDex Agar with Lecithin and Tween® 80 contains:
Dextrose 40.0gm
Pancreatic Digest of Casein 5.0gm
Peptic Digest of Animal Tissue 5.0gm
Agar 15.0gm
Lecithin 0.7gm
Tween® 80 5.0ml

Final pH 5.6 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store contact plate media (Cat. no. P750 and P890) at 15-30ºC. and other plated media (Cat no. W257) at 2-8ºC. away from direct light. Media should not be used if here are any signs of contamination, deterioration, discoloration, or if the expiration date has passed. Product is light and temperature sensitive. Protect from freezing.

PRECAUTIONS

PROCEDURE

Before Use of Plates:
It is possible that variation in temperature and pressure during shipping and storage may cause condensation on the innermost bag surrounding the product. If condensation of the packaging or plates is observed, remove the plates from the innermost packaging in a sterile environment and allow them to dry for 10-15 minutes before use.

SabDex Sedimentation Plate Method of Use:
Place a new paper towel or parchment paper at the location where the sample is to be taken. Place the petri dish on the paper and expose the agar by removing the lid. In order to prevent sediment from falling into the lid, place the lid, without inversion on the paper. Expose the agar for 15 minutes, replace the lid and incubate according to laboratory procedures. Consult listed references for information on these methods.(1-4,7,8)

SabDex Contact Plate Method of Use:
Select a surface to test. Sample the surface by firmly pressing the agar against the test area, using the thumb and second finger to hold the plate, and the first finger to press firmly and evenly on the base. The same amount of pressure should be used for each sample. Do not move the plate laterally, as this spreads contaminants across the agar surface. A rolling motion may be used when slightly curved surfaces are sampled. Areas to be assayed may by divided into grids or sections, and samples taken from specific areas within the divisions. Incubate exposed plates at 25 to 35ºC. for 1-7 days, as required. Consult listed references for information on these methods.(1-4,7, 8)

INTERPRETATION OF RESULTS

Sedimentation Method:
After incubation, the colonies are counted, and the results are expressed as the number of colonies per square foot per minute. For Cat. no. P750 and P890, the petri plate area is approximately 60cm2, the number counted colonies can be divided by 60 to provide the estimated number of colonies per square centimeter.(1-3,8) Similarly, for Cat. no. W257, the number of counted colonies can be divided by 100 to provide the estimated number of colonies per square centimeter.

Contact Plate Method:
After incubation, the colonies are counted, and the results are recorded as the number of colonies per centimeter squared or as colonies per plate.(1-3,8)

LIMITATIONS

Storage at 2-8°C may be used, but may result in moisture build-up inside sealed packs.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, microscope slides and coverslips, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms** Inoculation Method* Incubation Results
Time Temperature Atmosphere
Candida albicans***
ATCC® 10231
A 1-5 days 20-25°C Aerobic Growth
Aspirgillus brasiliensis
ATCC® 16404
G 1-5 days 20-25°C Aerobic Growth
Trichophyton mentagrophytes
ATCC® 9533
G 1-5 days 20-25°C Aerobic Growth

** Consult appropriate regulatory agency for user QC requirements.

*** Cat. no. P890 is incubated 35°C.

User Quality Control

Physical Appearance

Sabouraud Dextrose (SabDex) with Lecithin and Tween®, Irradiated should appear translucent, and light amber in color.

SabDex Agar

Uninoculated plate of Sabouraud Dextrose Agar, Irradiated.

REFERENCES

1. American Public Health Association. Standard Methods for the Examination of Dairy Products, APHA, Washington, D.C.

2. Vanderzant, C. and D.F. Splittstoesser, (ed.). 2001 Compendium of Methods for the Microbiological Examination of Foods, 4th ed. APHA, Washington, D.C.

3. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.

4. MacFaddin, J.F. Biochemical Tests for Identification of Medical Bacteria, Lipincott Williams & Wilkins, Philadelphia, PA.

5. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

6. Sabouraud, R. 1892. Ann. Dermatol. Syphil.; 3:1061.

7. The Official Compendia of Standards. USP-NF. United States Pharmacopeial Convention, Rockville, MD.

8. Association of Official Analytical Chemists. Official Methods of Analysis, AOAC, Washington, D.C.

ATCC is a registered trademark of the American Type Culture Collection.
Tween is a registered trademark of ICI Americas, Inc.

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