Hardy Diagnostics HUGO


Cat. no. D12 Saline, 0.45%, 11.75x75mm Tube, 1.8 - 2.2ml 100 tubes/box
Cat. no. U159 Saline, 0.45%, 1L Polycarbonate Bottle, 1000ml 10 bottles/box
Cat. no. D185 Saline, 0.85%, 11.75x75mm Tube, 1.8 - 2.2ml 100 tubes/box
Cat. no. R45 Saline, 0.85%, 13x100mm Tube, 2ml 20 tubes/box
Cat. no. R55 Saline, 0.85%, 13x100mm EP Glass Tube, 2ml 20 tubes/box
Cat. no. R47 Saline, 0.85%, 13x100mm Tube, 4ml 20 tubes/box
Cat. no. K248 Saline, 0.85%, 15x103mm Tube, 1ml 20 tubes/box
Cat. no. K59 Saline, 0.85%, 16x100mm Tube, 5ml 20 tubes/box
Cat. no. K52 Saline, 0.85%, 16x100mm Tube, 9ml 20 tubes/box
Cat. no. K53 Saline, 0.85%, 16x125mm Tube, 9.9ml 20 tubes/box
Cat. no. K51 Saline, 0.85%, 16x125mm Tube, 10ml 20 tubes/box
Cat. no. K58 Saline, 0.85%, 20x125mm Tube, 10ml 20 tubes/box
Cat. no. U155 Saline, 0.85%, 125ml Polypropylene Bottle, 100ml 12 bottles/box
Cat. no. U59 Saline, 0.85%, 500ml PC Bottle 500ml Bottle
Cat. no. U157 Saline, 0.85%, 1L Polycarbonate Bottle, 1000ml 10 bottles/box


Hardy Diagnostics Saline products are recommended for procedures that require the use of an isotonic diluent. It is also recommended for use in preparing test suspensions of organisms.


Saline is useful as a diluent to maintain cell integrity and viability because it lacks properties that may interfere with biochemical reactions and/or antibiotic susceptibility tests. The concentration of sodium chloride in 0.85% (normal) Saline provides osmotic protection for microbial cells. Normal saline is used for preparing microbial suspensions when it is necessary to deliver a set number of microbes to an identification test battery, to antimicrobial agents, or to growth media used for disk susceptibility testing. It is also used in the preparing of stock solutions and serial dilutions of antimicrobial agents. The Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS) recommends the use of 0.85% Saline to adjust the turbidity of bacterial suspensions to help maintain cell integrity and viability.(1,2) Saline, 0.45% is the isotonic diluent recommended for use with the Vitek® System.


Ingredients per liter of deionized water:*

Saline, 0.45%:
Sodium Chloride 4.5gm

Saline, 0.85%:
Sodium Chloride 8.5gm

Final pH of both solutions is 6.5 +/- 1.0 at 25ºC.**

* Adjusted and/or supplemented as required to meet performance criteria.

** Final pH of K59 is 5.5-7.0 at 25ºC which is compatible with the API® and Micro ID® enteric bacterial identification strips.

** Final pH of D12 is 5.5-7.2 at 25ºC and is consistent with Vitek®/bioMerieux specifications for this product.

** Final pH of D185 is 5.5-7.2 at 25ºC.

** Final pH of U59 is 6.3-7.2 at 25ºC.

** Final pH of K248 is 5.5-7.2 at 25ºC.


Storage: Upon receipt, store at 2-30°C away from direct light. Saline should not be used if there are any signs of deterioration (evaporation, discoloration), contamination, or if the expiration date has passed. Protect from excessive heat.



Specimen Collection: Specimen collection is not applicable since saline is not used in the primary isolation of microbes from clinical specimens.

Method of Use: Solutions for inoculation should be prepared by inoculating the diluent with one to five isolated colonies of test organism. The suspension should be adjusted to the concentration of the appropriate McFarland inoculum standard.

See the Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS) publications "Standards for Antimicrobial Susceptibility Testing" for details on use of normal saline for Kirby-Bauer sensitivity standardization.(1,2)

Inoculation Procedure for Saline, 0.45% (Cat. no. D12) intended for Vitek®/bioMerieux System: It is recommended by the manufacturer of the luminometer to "blank" each tube prior to inoculation.

Quality and thickness of the glass may vary and cause differences int the luminometer's readings. For increased accuracy, after the inoculation of the tube, it is recommended to insert the tube back into the luminometer with the same orientation as when the tube was originally blanked.


Saline lacks nutrient substances, and therefore does not support microbial growth. However, nutrient carry-over may allow growth to continue.

Failure to blank each tube individually before inoculation and orient the tube consistently after inoculation may lead to erroneous turbidity readings on the luminometer.


Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.


Saline is not a growth medium. It is tested for sterility only.

User Quality Control


Saline should appear clear and colorless.

Saline, 0.45%, 2mL

Saline, 0.45%, 2mL (Cat. no. R45).


1. Performance Standards for Antimicrobial Disk Susceptibility Tests, 6th ed., M2-A11, Vol. 33, No. 1. 2013. Clinical Laboratory Standards Institute (CLSI - formerly NCCLS), Villanova, PA.

2. Methods for Dilution Antimicrobial Susceptibility Test For Bacteria That Grow Aerobically, M7-current edition. Clinical Laboratory Standards Institute (CLSI - formerly NCCLS), Villanova, PA.

3. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

4. Versalovic, J., et al. Manual of Clinical Microbiology. American Society for Microbiology, Washington, D.C.

5. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

6. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

API® and Vitek® are registered trademarks of bioMerieux, France.
Micro ID® is a registered trademark of Organon Teknika.