STOCK ORGANISM LIST and Maintenance program at Hardy Diagnostics

Listed below are the control organisms in common usage at Hardy Diagnostics. All organisms can be purchased through Hardy Diagnostics from MicroBioLogics (MBL) in lyophilized form. See the keyword "MBL Organism List" for a complete listing of organisms. Visit www.hardydiagnostics.com to view the entire listing of organisms on the Hardy Web catalog.

The organism list below represents the organisms routinely maintained at Hardy Diagnostics. The Maintenance Methods listed are those used at Hardy Diagnostics to preserve the viability of the organisms, while minimizing the chance of phenotypic alterations.

Organism (ATCC ® Number) Maintenance Code (see below)
Acinetobacter baumannii (19606) I
Aeromonas hydrophila (35654) B
Aspergillus brasiliensis formerly A. niger (16404) F
Bacillus cereus (13061) D
Bacillus subtilis (6633) D
Bacteroides distasonis (8503) E
Bacteroides fragilis (25285) E
Bacteroides levii (29147) E
Bacteroides uniformis (8492) E
Bacteroides vulgatus (8482) E
Bordetella pertussis (9340) H
Branhamella (Moraxella) catarrhalis (25240) C
Campylobacter jejuni (33291) E
Candida albicans (60193) for GC Media D
Candida albicans (10231) D
Candida glabrata (Vitek-986) D
Candida krusei (14243) D
Candida tropicalis (750) D
Citrobacter freundii (8090) I
Citrobacter freundii (33291) I
Clostridium novyi A (7659) E
Clostridium perfringens (13124) E
Clostridium sordelli (9714) E
Corynebacterium diphtheriae (13812) I
Corynebacterium pseudodiphtheriticum (10700) I
Cryptococcus neoformans (32045) F
Enterobacter aerogenes (13048) I
Enterobacter cloacae (23355) 1 D
Enterococcus casseliflavus (clinical) I
Organism (ATCC ® Number) Maintenance Code (see below)
Enterococcus faecalis (29212) 1 I
Enterococcus faecalis (51299) I
Enterococcus faecium (UCLA clinical strain 9562) I
Enterococcus gallinarum (clinical) I
Escherichia coli (25922) 1 I
Escherichia coli (35218) I
Escherichia coli 0157:H7 (35150) D
Flavobacterium meningosepticum (13253) D
Fusobacterium nucleatum (25586) E
Gardnerella vaginalis (14018) C
Haemophilus haemolyticus (33390) C
Haemophilus influenzae (10211) C
Haemophilus influenzae (49247) for HTM 1 C
Haemophilus influenzae (49766) for HTM 1 C
Haemophilus parahaemolyticus (10014) C
Haemophilus parainfluenzae (7901) C
Haemophilus paraphrophilus (29241) C
Klebsiella pneumoniae (13883) D
Klebsiella pneumoniae (13882) D
Organism (ATCC ® Number) Maintenance Code (see below)
Lactobacillus acidophilus (4356) D
Legionella bozemanii (33217) J
Legionella micadei (33204) J
Legionella pneumophila (33152) J
Leuconostoc paramesentroides (33313) B
Listeria monocytogenes (7644) D
Micrococcus spp. (clinical) D
Morganella morganii (25830) I
Mycobacterium fortuitum Group IV (6841) G
Mycobacterium genavense (clinical) G
Mycobacterium intracellulare Group III (13950) G
Mycobacterium kansasii Group I (12478) G
Mycobacterium phlei (11758) G
Mycobacterium scrofulaceum Group II (19981) G
Mycobacterium tuberculosis H37R (25177) G
Mycobacterium xenopi (19250) G
Mycoplasma hominis (23114) K
Mycoplasma pneumoniae (29085) K
Neisseria denitrificans (14686) C
Neisseria gonorrhoeae (19424) A
Neisseria gonorrhoeae (43069) A
Neisseria gonorrhoeae (49226) for GC Base 1 A
Neisseria meningitidis (13090) C
Neisseria sicca (9913) C
Nocardia asteroides (3308) L
Nocardia braziliensis (19296) L
Nocardia caviae (14629) L
Peptostreptococcus anaerobius (27337) E
Proteus mirabilis (12453) D
Proteus hauseri (13315) D
Pseudomonas aeruginosa (27853) 1 I
Pseudomonas cepacia (25416) D
Pseudomonas stutzeri (17588) D
Organism (ATCC ® Number) Maintenance Code (see below)
Salmonella paratyphi A (9150) D
Salmonella enterica (14028) D
Serratia marcescens (8100) D
Serratia marcescens (clinical strain-pigmented) D
Shigella flexneri (12022) D
Shigella sonnei (9290) D
Staphylococcus aureus (25913) D
Staphylococcus aureus (25923) 1 I
Staphylococcus aureus (33862) for camp test D
Staphylococcus aureus (CDC isolate no. 80) D
Staphylococcus aureus (33591) methicillin-resistant D
Staphylococcus aureus (43300) methicillin-resistant D
Staphylococcus epidermidis (12228) D
Staphylococcus saprophyticus (15305) D
Streptococcus agalactiae (12386) B
Streptococcus pneumoniae (6305) B
Streptococcus pneumoniae (49619) B
Streptococcus pyogenes (19615) B
Streptococcus viridans (clinical isolate) B
Trichophyton equinum (22443) F
Trichophyton megninii (12106) F
Trichophyton mentagrophytes (9533) F
Trichophyton rubrum (28188) F
Ureaplasma urealyticum (27618) K
Vibrio parahaemolyticus (V-H-1) D
Yersinia enterocolitica (9610) D

MAINTENANCE METHODS

The chart below provides a suggested protocol for the maintenance of the above stock organisms to ensure viability and prevent phenotypic alterations.

CODE MEDIA SUBCULTURE
EVERY
ATMOSPHERE
INCUBATION TEMP STORAGE
TEMP
REGENERATE
EVERY
A Chocolate Agar Plate 2 or 3 Days CO 2 35 o C. 35 o C. Week
B Blood Agar Slant 2 Weeks Aerobic 35 o C. 2-8 o C. Month
C Chocolate Agar Slant 2 Weeks CO 2 35 o C. 2-8 o C. Month
D Tryptic Soy Agar Plate 2 Weeks Aerobic 35 o C. 2-8 o C. Month
E Chopped Meat with H, K, and Iron 2 2 Weeks Aerobic 35 o C. 2-8 o C. Month
F Sabdex Agar Slant 1 Month Aerobic 15-30 o C. 15-30 o C. Month
G Lowenstein-Jensen Slant 1 Month CO 2 35 o C. 15-30 o C. Month
H Regan-Lowe Plate 2 Weeks Aerobic 35 o C. 2-8 o C. Month
I Tryptic Soy Agar Plate 1 Week Aerobic 35 o C. 2-8 o C. Month
J BCYE Agar Plate 2 Weeks Aerobic 35 o C. 2-8 o C. Month
K SP4 Base Broth 2 do not sub. CO 2 35 o C. -70 o C. Year
L Emmons Sabdex 1 Month Aerobic 15-30 o C. 15-30 o C. Month

1 - CLSI requires weekly subculturing and monthly regeneration of these organisms, when used for disk susceptibility testing. (5) Organisms used for routine quality control procedures, CLSI recommends that stock cultures be started from a new pellet every year and that a 'Stock Control' is prepared. A 'Working Control' is prepared each month from the 'Stock Control'. See the HUGO document, "Preparation and Maintenance of QC Stock Cultures".

2 - Organisms must be regenerated on solid non-selective media before subculturing to this broth medium.

For more information on QC organisms and performance testing, see the following keywords in the Hardy Diagnostics software program HUGO™:

"QC of Finished Product"

"Preparation and Maintenance of QC Stock Cultures"

"Stock Culture Media"

"Inoculation Procedures and Codes"

"The CLSI (NCCLS) Standard and Recommendations for User QC of Media"

ATCC is a registered trademark of the American Type Culture Collection.

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