TAT Broth Media

Cat. no. D077 TAT Broth, Dilu-Lok II™ Vial, 90ml 50 vials/case
Cat. no. K179 TAT Broth with Lecithin and Tween ® 80, 16x125mm Tube, 3ml 20 tubes/box
Cat. no. K251 TAT Broth, 20x125mm Tube, 9ml 20 tubes/box
Cat. no. U77 TAT Broth, 180ml Wide Mouth Jar, 90ml 12 jars/box
Cat. no. U78 TAT Broth, 4oz. Glass Bottle, 99ml 20 bottles/box
Cat. no. U87 TAT Broth, 4oz. Glass Bottle, 90ml 20 bottles/box
Cat. no. U88 TAT Broth, 180ml Wide Mouth Jar, 99ml 12 jars/box
Cat. no. U318 TAT Broth, 1 Liter Polypropylene Bottle, 490ml 10 bottles/box
Cat. no. U319 TAT Broth, 1 Liter Polypropylene Bottle, 1000ml 10 bottles/box

INTENDED USE

Hardy Diagnostics TAT (Tryptone-Azolectin-Tween ® ) Broth is recommended for use in cultivating microorganisms from highly viscous or gelatinous materials, such as salves and ointments. These media are especially adapted to the microbiological examination of non-sterile products from pharmaceutical, cosmetic and raw material or end-products, for the purpose of performing microbial enumeration studies.

This product is not intended to be used for the diagnosis of human disease.

SUMMARY

TAT (Tryptone-Azolectin-Tween ® ) Broth, also known as Casein-peptone Lecithin Polysorbate Broth, is recommended for sterility testing of viscous materials, such as salves, creams and ointments, and is especially suited to the sterility testing of cosmetics. (1-3) Cosmetics and topical pharmaceutical products are easily subjected to contamination during the manufacturing process and through continued use by consumers. (2,3) Consequently, these products contain preservatives for self-sterilization against vegetative bacteria, yeast, and mold.

Hardy Diagnostics TAT Broth formulations are enriched to isolate and cultivate microorganisms from viscous materials. These media contain pancreatic digest of casein, which provides nitrogen, vitamins, amino acids, and carbon. Lecithin (azolectin) and Tween ® 20 neutralize common preservatives in cosmetics or pharmaceutical products, thereby permitting the growth of contaminating flora. The relatively high casein content provides optimal conditions for spore germination and regeneration of damaged cells. Lecithin and polysorbate 20 inactivate many antimicrobial compounds.

Hardy Diagnostics TAT Broth with Lecithin and Tween ® 80 contains additional lecithin and Tween ® 80 to further neutralize germicidal or disinfectant residues on highly viscous materials. Neutralization of these residues reduces their inhibitory effects. Quaternary ammonia compounds are neutralized by lecithin while phenolic disinfectants and hexachlorophene are neutralized by Tween ® . Together, lecithin and Tween ® 80 neutralize ethanol.

FORMULA

Ingredients per liter of deionized water:*

Pancreatic Digest of Casein 20.0gm
Lecithin 5.0gm
Tween ® 20 40.0ml

In addition, TAT Broth with Lecithin and Tween ® 80 also contains:

Lecithin 0.7gm
Tween ® 80 5.0ml

Final pH 7.2 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store Cat. no. K179 at 2-8ºC. away from direct light. Upon receipt store Cat. nos. D077, K251, U77, U78, U87, U88, U318 and U319 at 2-30ºC. away from direct light. Media should not be used if there are any signs of deterioration, discoloration, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

PROCEDURE

For a complete discussion on sterility testing and culture, consult the appopriate reference method. (1-3)

1. Add 1.0gm or 1ml of an undiluted sample to 40ml of complete medium and agitate to obtain an even suspension.

2. Incubate at 35ºC. for 18-48 hours.

INTERPRETATION OF RESULTS

Tubes or bottles exhibiting growth in the form of turbidity should be subcultured for further identification.

LIMITATIONS

Since the nutritional requirements of organisms vary, some strains may be encountered that fail to grow or grow poorly on this medium.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, swabs, applicator sticks, other culture media, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
TAT Broth & TAT Broth with Lecithin and Tween ® 80:
Salmonella enterica
ATCC ® 14028
A 18-48hr 35°C Aerobic Growth
Pseudomonas aeruginosa
ATCC ® 27853
A 18-48hr 35°C Aerobic Growth
Bacillus subtilis
ATCC ® 6633
A 18-48hr 35°C Aerobic Growth
Staphylococcus aureus
ATCC ® 25923
A 18-48hr 35°C Aerobic Growth
TAT Broth (Cat. no. U318 and U319):
Staphylococcus aureus
ATCC ® 6538
J 3-5 days 30-35°C Aerobic Growth
Pseudomonas aeruginosa
ATCC ® 9027
J 3-5 days 30-35°C Aerobic Growth
Bacillus subtilis
ATCC ® 6633
J 3-5 days 30-35°C Aerobic Growth
Escherichia coli
ATCC ® 8739
J 3-5 days 30-35°C Aerobic Growth
Salmonella enterica
ATCC ® 14028
J 3-5 days 30-35°C Aerobic Growth

USER QUALITY CONTROL

Physical Appearance

TAT Broth should appear slightly opalescent, and light amber in color with a slight precipitate. TAT Broth with Lecithin and Tween ® 80 should appear slightly opalescent, and light to medium amber in color with a slight precipitate

REFERENCES

1. The Official Compendia of Standards. USP-NF. United States Pharmacopeial Convention, Rockville, MD.

2. Orth, D.S. 1993. Handbook of cosmetic microbiology. Marcel Dekker, Inc. New York, NY.

3. U.S. Food and Drug Administration. Bacteriological Analytical Manual. AOAC, Arlington, VA.
http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006949.htm.

ATCC is a registered trademark of the American Type Culture Collection.
Tween is a registered trademark of ICI Americas, Inc.

041816gr