THAYER MARTIN, MODIFIED

Cat. no. E30 Thayer Martin, Modified, 15x100mm Plate, 18ml 10 plates/bag
Cat. no. E130BX Thayer Martin, Modified, with Vancomycin, 15x100mm Plate, 18ml 100 plates/box
Cat. no. E450 Thayer Martin, Modified, 50x75mm Square Pill Pocket Plate, 16ml 10 plates/bag
Cat. no. J72 Chocolate / MTM (Modified Thayer Martin), 15x100mm Biplate, 10ml/10ml 10 plates/bag

INTENDED USE

Hardy Diagnostics Modified Thayer Martin (MTM) is a selective medium used in qualitative procedures for the isolation of Neisseria gonorrhoeae with suppression of most other gram-negative diplococci, gram-negative bacilli, gram-positive organisms, and yeast.

SUMMARY

Thayer and Martin (1964) reported an improvement of the Chocolate Agar formulation by the addition of antimicrobics which suppressed the growth of some contaminating organisms but which allowed N. gonorrhoeae and N. meningitidis to grow. In 1970, trimethoprim lactate was shown to be of value in the suppression of Proteus spp. The resulting medium is called Modified Thayer Martin.

FORMULA

Ingredients per liter of deionized water:**

Proteose Peptone No. 3 15.0gm
Hemoglobin, Bovine 10.0gm
Sodium Chloride 5.0gm
Dipotassium Phosphate 4.0gm
Monopotassium Phosphate 1.0gm
Corn Starch 1.0gm
Colistin 7.5mg
Trimethoprim Lactate* 5.0mg
Vancomycin 3.0mg
Nystatin 1,250U
KoEnzyme Enrichments 10.0ml
Agar 12.0gm

Final pH 7.2 +/- 0.2 at 25ºC.

*Thayer Martin, Modified with Vancomycin (Cat. no. E130BX) does not contain Trimethoprim Lactate.

** Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), hemolysis, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

PROCEDURE

Specimen Collection: Specimens should be submitted directly to the laboratory without delay and protected from excessive heat and cold. If there is to be a delay in processing, specimens should be inoculated into an appropriate transport medium such as Amies with charcoal. Specimens must be transported at ambient temperatures (15-30 degrees C). Do not refrigerate. Refer to listed references for more information on specimen collection and transport.(1-3)

Method of Use: Bring media to room temperature before use. Inoculate media and streak to obtain isolated colonies. Incubate 24-48 hours at 35ºC. in 5-10% CO2. Some strains may require up to 72 hours to appear.

Square Pill Pocket Plate: After inoculation, place one CO2 tablet in the pill pocket and place plate in a sealed zip bag. Do not invert the plate. Proceed with incubation parameters as outlined above.

INTERPRETATION OF RESULTS

Neisseria gonorrhoeae appears as small, grayish-white to colorless mucoid colonies. N. meningitidis forms similar colonies to N. gonorrhoeae, but larger and blue-gray.

An oxidase test may be performed from the primary medium for presumptive identification.

LIMITATIONS

Some diagnostic tests may be performed with the primary media. However, additional tests including gram stain and biochemical testing should be performed on pure cultures for complete identification. For more information, see appropriate references.

The agents in selective media may inhibit some strains of desired species or permit the growth of species they were designed to inhibit. Therefore, specimens cultured on selective media should also be cultured on non-selective media to obtain additional information and to help insure recovery of potential pathogens. Some strains of vancomycin-sensitive N. gonorrhoeae may be inhibited on this media. To avoid this inhibition, see "Martin Lewis with Lincomycin".

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Thayer Martin, Modified:
Neisseria gonorrhoeae
ATCC® 43069
A 24-48hr 35°C CO2** Growth
Neisseria meningitidis
ATCC® 13090***
A 24-48hr 35°C CO2** Growth
Staphylococcus epidermidis
ATCC® 12228
B 24-48hr 35°C CO2** Partial to complete inhibition
Proteus mirabilis
ATCC® 43071
B 24-48hr 35°C CO2** Partial to complete inhibition; no swarming
Escherichia coli
ATCC® 25922***
B 24-48hr 35°C CO2** Partial to complete inhibition
Candida albicans
ATCC® 60193***
B 24-48hr 35°C CO2** Partial to complete inhibition
Neisseria sicca
ATCC® 9913***
B 24-48hr 35°C CO2** Inhibited


Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Thayer Martin, Modified, with Vancomycin (Cat. no. E130BX):
Neisseria gonorrhoeae
ATCC® 43069
A 24-48hr 35°C CO2** Growth
Neisseria meningitidis
ATCC® 13090***
A 24-48hr 35°C CO2** Growth
Staphylococcus epidermidis
ATCC® 12228
B 24-48hr 35°C CO2** Partial to complete inhibition
Escherichia coli
ATCC® 25922***
B 24-48hr 35°C CO2** Partial to complete inhibition
Candida albicans
ATCC® 60193***
B 24-48hr 35°C CO2** Partial to complete inhibition
Neisseria sicca
ATCC® 9913***
B 24-48hr 35°C CO2** Inhibited

** Atmosphere of incubation is enriched with 5-10% CO2.

*** To be used only by commercial media manufacturers.

User Quality Control

PHYSICAL APPEARANCE

Thayer Martin, Modified Agar should appear opaque, and brown in color.

N. gonorrhoeae growing on Thayer Martin, Modified Agar

Neisseria gonorrhoeae (ATCC® 43069) colonies growing on Thayer Martin, Modified Agar (Cat. no. E30). Incubated in CO2 for 48 hours at 35ºC.

S. epidermidis inhibited on Thayer Martin, Modified Agar

Staphylococcus epidermidis (ATCC® 12228) growth inhibited on Thayer Martin, Modified Agar (Cat. no. E30). Incubated in CO2 for 48 hours at 35ºC.

REFERENCES

1. Versalovic, J., et al. Manual of Clinical Microbiology. American Society for Microbiology, Washington, D.C.

2. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

3. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

4. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

5. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.


ATCC is a registered trademark of the American Type Culture Collection.

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