TRYPTIC SOY AGAR (TSA) MEDIA, IRRADIATED, USP
|Cat. no. G267||TSA, Irradiated, USP, 15x100mm Plate, SterEmTM, 18ml, Triple Bagged*||2 plates/bag|
|Cat. no. W1670||TSA, Irradiated, USP, 15x100mm Plate, 26ml, Triple Bagged*||10 plates/bag|
|Cat. no. W3670||TSA, Irradiated, USP, 15x100mm Plate, 34ml, Triple Bagged*||10 plates/bag|
|Cat. no. W3770R||TSA, Irradiated, USP, 15x100mm Red Plate, 34ml, Triple Bagged*||10 plates/bag|
|* A fourth sterile sample bag is included for packaging after the sample is collected.|
Hardy Diagnostics Irradiated Tryptic Soy Agar (TSA), USP is recommended for use as a general growth medium for the detection and enumeration of microorganisms.
This product is not intended to be used for the diagnosis of human disease.
The formulation of Tryptic Soy Agar, USP is prepared according to the United States Pharmacopeia standard formula for Soybean-Casein Digest Agar Medium.(2)
Hardy Diagnostics Tryptic Soy Agar (TSA) contains digests of soybean meal and casein which provide amino acids and other nitrogenous compounds to promote the growth of a variety of microorganisms. Sodium chloride is added to maintain the osmotic equilibrium. Agar is the solidifying agent.
The plates are triple bagged and sterilized by irradiation to promote a higher sterility assurance level.
Ingredients per liter of deionized water:*
|Pancreatic Digest of Casein||15.0gm|
|Peptic Digest of Soybean Meal||5.0gm|
Final pH 7.3 +/- 0.2 at 25°C.
* Adjusted and/or supplemented as required to meet performance criteria.
STORAGE AND SHELF LIFE
Storage: Store Cat. no. G267 at 2-8°C., Cat. no. W1670, W3670, and W3770R at 15-30°C., and store all media away from direct light. Media should not be used if there are any signs of contamination, deterioration, discoloration, or if the expiration date has passed. Product is light and temperature sensitive. Protect from freezing.
Before Use of Plates:
It is possible that variation in temperature and pressure during shipping and storage may cause condensation on the innermost bag surrounding the product. If condensation of the packaging or plates is observed, remove the plates from the innermost packaging in a sterile environment and allow them to dry for 10-15 minutes before use.
For air sampling, consult USP <1116>.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.
|Test Organisms||Inoculation Method*||Incubation||Results|
All TSA irradiated media is held for 7 days to confirm the media meets our validated Sterility Assurance Level (SAL) of 10-5 for W1670, G267, W3670, and W3770R.
USER QUALITY CONTROL
Tryptic Soy Agar (TSA) Agar should appear translucent, and light amber in color. Tryptic Soy Agar (TSA) Agar with Lecithin and Tween® should appear clear to slightly hazy and light amber in color.
Uninoculated plate of Tryptic Soy Agar (Cat. no. W1670).
1. American Public Health Association. 2012. Standard Methods for the Examination of Water and Wastewater, 22nd ed. APHA, Washington, D.C.
2. The Official Compendia of Standards. USP General Chapter <61> Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests. USP-NF. United States Pharmacopeial Convention Inc., Rockville, MD.
3. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.
ATCC is a registered trademark of the American Type Culture Collection.