TSB (Tryptic Soy Broth) with Lecithin and Tween® 80
|Cat. no. K134||TSB with Lecithin and Tween® 80, 20x125mm Tube, 9ml||20 tubes/box|
|Cat. no. K292||TSB with Lecithin and Tween® 80, 20x125mm Tube, 18ml||20 tubes/box|
|Cat. no. U229||TSB with Lecithin and Tween® 80, 500ml Polypropylene Bottle, 500ml||10 bottles/box|
|Cat. no. U292||TSB with Lecithin and Tween® 80, 180ml Wide Mouth Jar, 99ml||12 jars/box|
|Cat. no. U306||TSB with 0.7% Lecithin and 5% Tween® 80, 500ml Polypropylene Bottle, 500ml||10 bottles/box|
|Cat. no. U423||TSB with Lecithin and Tween® 80, 20ml Serum Vial, 10ml||50 vials/box|
|Cat. no. U424||TSB with Lecithin and Tween® 80, 200ml Bottle with Septum, 150ml||10 bottles/box|
|Cat. no. U425||TSB with Lecithin and Tween® 80, 500ml Bottle with Septum, 300ml||10 bottles/box|
|Cat. no. X39||TSB with Lecithin and Tween® 80, 50ml Centrifuge Tube, 18ml||25 tubes/box|
Hardy Diagnostics Tryptic Soy Broth (TSB) with Lecithin and Tween® 80 is recommended for the cultivation of a wide variety of fastidious and nonfastidious microorganisms from nonclinical specimens, when the neutralization of disinfectants is desired. This medium can also be used for sterility testing and to test the microbial content of water.
This product is not intended to be used for the diagnosis of human disease.
The formulation of Tryptic Soy Broth with Lecithin and Tween® 80 is prepared according to the United States Pharmacopeia (USP) standard formula for Soybean-Casein Digest Broth.(3) It is also included in approved procedures in the Compendium of Methods for the Microbiological Examination of Foods and Standard Methods for the Examination of Water and Wastewater.(1,2)
Hardy Diagnostics Tryptic Soy Broth (TSB) with Lecithin and Tween® 80 contains digests of soybean meal and casein which provide amino acids and other nitrogenous compounds to support microbial growth. Sodium chloride is added to maintain cellular osmotic equilibrium. Glucose provides an energy source. Lecithin and Tween® 80 are added to the formulation to neutralize germicidal or disinfectant residues. Neutralization of these residues reduces their inhibitory effect which would ultimately result in the lowering of the microbial count. Quaternary ammonia compounds are neutralized by lecithin while phenolic disinfectants and hexachlorophene are neutralized by Tween® 80. Together, lecithin and Tween® 80 neutralize ethanol.(1,2)
Ingredients per liter of deionized water:*
|Pancreatic Digest of Casein||17.0gm|
|Peptic Digest of Soybean Meal||3.0gm|
Final pH 7.3 +/- 0.2 at 25ºC.
* Adjusted and/or supplemented as required to meet performance criteria.
STORAGE AND SHELF LIFE
Storage: Upon receipt store at 2-25ºC. away from direct light. Media should not be used if there are any signs of deterioration, discoloration, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.
Specimen Collection: Consult listed references for information on specimen collection.(1-3)
Method of Use: Allow media to warm to room temperature prior to inoculation. Consult listed references for information concerning testing procedures.(1-3)
Inoculate and incubate tubes, jars and/or bottles per techniques and procedures established via laboratory policy.
INTERPRETATION OF RESULTS
Positive growth is indicated by the media becomming cloudy or turbid.
Consult listed references for more detailed information concerning positive growth interpretation.(1-3)
Results can be uninterpretable or misleading unless a statistical method for monitoring is designed.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as loops, swabs, applicator sticks, other culture media, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.
|Test Organisms||Inoculation Method*||Incubation||Results|
|Cat. no. K134, K292, U229, U292, U423, U424, U425, X39|
|Cat. no. U306|
USER QUALITY CONTROL
Hardy Diagnostics Tryptic Soy Broth (TSB) with Lecithin and Tween®80 should appear clear, and light amber in color.
1. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.
2. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.
3. United States Pharmacopoeia and National Formulary (USP-NF). Rockville, MD: United States Pharmacopeial Convention.
ATCC is a registered trademark of the American Type Culture Collection.
Tween is a registered trademark of ICI Americas, Inc.