TSB (Tryptic Soy Broth) with Lecithin and Tween® 80

Cat. no. K134 TSB with Lecithin and Tween® 80, 20x125mm Tube, 9ml 20 tubes/box
Cat. no. K292 TSB with Lecithin and Tween® 80, 20x125mm Tube, 18ml 20 tubes/box
Cat. no. U134 TSB with Lecithin and Tween® 80, 180ml Wide Mouth Jar, 90ml 12 jars/box
Cat. no. U229 TSB with Lecithin and Tween® 80, 500ml Polypropylene Bottle, 500ml 10 bottles/box
Cat. no. U292 TSB with Lecithin and Tween® 80, 180ml Wide Mouth Jar, 99ml 12 jars/box
Cat. no. U306 TSB with 0.7% Lecithin and 5% Tween® 80, 500ml Polypropylene Bottle, 500ml 10 bottles/box
Cat. no. U423 TSB with Lecithin and Tween® 80, 20ml Serum Vial, 10ml 50 vials/box
Cat. no. U424 TSB with Lecithin and Tween® 80, 200ml Bottle with Septum, 150ml 10 bottles/box
Cat. no. U425 TSB with Lecithin and Tween® 80, 500ml Bottle with Septum, 300ml 10 bottles/box
Cat. no. X39 TSB with Lecithin and Tween® 80, 50ml Centrifuge Tube, 18ml 25 tubes/box

INTENDED USE

Hardy Diagnostics Tryptic Soy Broth (TSB) with Lecithin and Tween® 80 is recommended for the cultivation of a wide variety of fastidious and nonfastidious microorganisms from nonclinical specimens, when the neutralization of disinfectants is desired. This medium can also be used for sterility testing and to test the microbial content of water.

This product is not intended to be used for the diagnosis of human disease.

SUMMARY

The formulation of Tryptic Soy Broth with Lecithin and Tween® 80 is prepared according to the United States Pharmacopeia (USP) standard formula for Soybean-Casein Digest Broth.(3) It is also included in approved procedures in the Compendium of Methods for the Microbiological Examination of Foods and Standard Methods for the Examination of Water and Wastewater.(1,2)

Hardy Diagnostics Tryptic Soy Broth (TSB) with Lecithin and Tween® 80 contains digests of soybean meal and casein which provide amino acids and other nitrogenous compounds to support microbial growth. Sodium chloride is added to maintain cellular osmotic equilibrium. Glucose provides an energy source. Lecithin and Tween® 80 are added to the formulation to neutralize germicidal or disinfectant residues. Neutralization of these residues reduces their inhibitory effect which would ultimately result in the lowering of the microbial count. Quaternary ammonia compounds are neutralized by lecithin while phenolic disinfectants and hexachlorophene are neutralized by Tween® 80. Together, lecithin and Tween® 80 neutralize ethanol.(1,2)

FORMULA

Ingredients per liter of deionized water:*

Pancreatic Digest of Casein 17.0gm
Sodium Chloride 5.0gm
Tween® 80 5.0gm
Peptic Digest of Soybean Meal 3.0gm
Dextrose 2.5gm
Dipotassium Phosphate 2.5gm
Lecithin 0.7gm

Final pH 7.3 +/- 0.2 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-25ºC. away from direct light. Media should not be used if there are any signs of deterioration, discoloration, contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

PROCEDURE

Specimen Collection: Consult listed references for information on specimen collection.(1-3)

Method of Use: Allow media to warm to room temperature prior to inoculation. Consult listed references for information concerning testing procedures.(1-3)

Inoculate and incubate tubes, jars and/or bottles per techniques and procedures established via laboratory policy.

INTERPRETATION OF RESULTS

Positive growth is indicated by the media becomming cloudy or turbid.

Consult listed references for more detailed information concerning positive growth interpretation.(1-3).

LIMITATIONS

Results can be uninterpretable or misleading unless a statistical method for monitoring is designed.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, swabs, applicator sticks, other culture media, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Cat. no. K134, K292, U134, U229, U292, U423, U424, U425, X39
Staphylococcus aureus
ATCC® 25923
A 24hrs 35°C Aerobic Growth
Aspergillus brasiliensis
ATCC® 16404
G 1-5 days 20-25°C Aerobic Growth
Escherichia coli
ATCC® 25922
A 24hrs 35°C Aerobic Growth
Cat. no. U306
Staphylococcus aureus
ATCC® 25923
A 24hrs 35°C Aerobic Growth
Aspergillus brasiliensis
ATCC® 16404
G 1-5 days 20-25°C Aerobic Growth
Pseudomonas aeruginosa
ATCC® 9027
A 24hrs 35°C Aerobic Growth
Candida albicans
ATCC® 10231
A 24-48hrs 35°C Aerobic Growth

USER QUALITY CONTROL

Physical Appearance

Hardy Diagnostics Tryptic Soy Broth (TSB) with Lecithin and Tween® 80 should appear clear, and light amber in color.

REFERENCES

1. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.

2. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.

3. United States Pharmacopoeia and National Formulary (USP-NF). Rockville, MD: United States Pharmacopeial Convention.


ATCC is a registered trademark of the American Type Culture Collection.
Tween is a registered trademark of ICI Americas, Inc.

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