TRYPTIC SOY BROTH (TSB), USP
|Cat. no. K82||TSB, USP, 16x125mm Tube, 10ml||20 tubes/box|
|Cat. no. K82BX||TSB, USP, 16x125mm Tube, 10ml||80 tubes *ReadyRack|
|Cat. no. K83||TSB, USP, 20x125mm Tube, 15ml||20 tubes/box|
|Cat. no. K182||TSB, USP, 16x125mm Tube with Hungate Septum Cap, 10ml||20 tubes/box|
|Cat. no. K380||TSB, USP, 20x150mm Tube, 20ml||100 tubes/box|
|Cat. no. K380BX||TSB, USP, 20x150mm Tube, 20ml||80 tubes *ReadyRack|
|Cat. no. U38||TSB, USP, 20ml Serum Vial, 20ml||50 vials/box|
|Cat. no. U42||TSB, USP, 4oz. Glass Bottle, 100ml||20 bottles/box|
|Cat. no. U44||TSB, USP, 2oz. Glass Bottle, 50ml||24 bottles/box|
|Cat. no. U46||TSB, USP, 100ml Serum Bottle, 100ml||20 bottles/box|
|Cat. no. U56||TSB, USP, 100ml Serum Bottle, 100ml, Unlabeled||20 bottles/box|
|Cat. no. U62||TSB, USP, 500ml Glass Bottle, 500ml||500ml bottle|
|Cat. no. U65||TSB, USP, 500ml Polycarbonate Bottle, 500ml||500ml bottle|
|Cat. no. U67||TSB, USP, 1L Polycarbonate Bottle, 1000ml||10 bottles/box|
|Cat. no. U71||TSB, USP, 180ml Wide Mouth Jar, 100ml||12 jars/box|
|Cat. no. U80||TSB, USP, 500ml Wide Mouth Polycarbonate Bottle with Cap, 225ml||10 bottles/box|
|Cat. no. U82||TSB, USP, 20ml Serum Vial, 15ml||50 vials/box|
|Cat. no. U100||TSB, USP, 100ml Bag, 100ml||10 bags/box|
|Cat. no. U133||TSB, USP, 180ml Wide Mouth Jar, 90ml||12 jars/box|
|Cat. no. U141||TSB, USP, 125ml Polycarbonate Bottle with Septum Cap, 100ml||16 bottles/box|
|Cat. no. U171||TSB, USP, 250ml Square Polycarbonate Bottle, 160ml||12 bottles/box|
|Cat. no. U223||TSB, USP, 180ml Wide Mouth Jar, 25ml||12 jars/box|
|Cat. no. U242||TSB, USP, 16oz. Glass Bottle, 400ml||12 bottles/box|
|Cat. no. U267||TSB, USP, 100ml Serum Bottle, 50ml||20 bottles/box|
|Cat. no. U271||TSB, USP, 16oz. Glass Bottle with Septum Cap, 400ml||12 bottles/box|
|Cat. no. U274||TSB, USP, 500ml Glass Bottle, 500ml||10 bottles/box|
|Cat. no. U316||TSB, USP, 8oz. Wide Mouth Jar, 180ml||12 jars/box|
|Cat. no. U421||TSB, USP, 500ml Glass Bottle with Septum Cap, 300ml||10 bottles/box|
|Cat. no. U435||TSB, USP, 8oz. Glass Bottle, 200ml||12 bottles/box|
|Cat. no. U8210||TSB, USP, 20ml Serum Vial, 10ml||50 vials/box|
*ReadyRack is a plastic rack designed for cleanroom use and can be rinsed with alcohol.
Hardy Diagnostics Tryptic Soy Broth is recommended for use as a general purpose medium for the isolation and cultivation of a wide variety of bacteria and fungi. The U.S. Pharmacopeia National Formulary (USP) describes its use for sterility testing.(1)
This product is not intended to be used for the diagnosis of human disease.
Tryptic Soy Broth is widely used for the cultivation of microorganisms from environmental sources; supporting the growth of the majority of bacteria and fungi. Tubes of this medium may be used for preparing dilutions of organism for colony counts and preparation of standard inocula. Tryptic Soy Broth is also recommended for use in sterility testing for the detection of contamination with low incidence fungi and aerobic bacteria.(1)
Tryptic Soy Broth, also known as Soybean-Casein Digest, conforms to the formula given by the U.S. Pharmacopeia.(1) This medium contains digests of soybean meal and casein, which provide amino acids and other nitrogenous substances, making it a highly nutritious medium for a variety of organisms. Sodium chloride is added to maintain the osmotic equilibrium. Dextrose is incorporated as an energy source. The dipotassium phosphate is included in the formulation as a buffer to maintain the pH.
Ingredients per liter of deionized water:*
|Pancreatic Digest of Casein||17.0gm|
|Papaic Digest of Soybean Meal||3.0gm|
Final pH 7.3 +/- 0.2 at 25°C.
* Adjusted and/or supplemented as required to meet performance criteria.
STORAGE AND SHELF LIFE
Storage: Upon receipt store at 2-25°C. away from direct light. Media should not be used if there are any signs of deterioration (discoloration), contamination, or if the expiration date has passed. Protect from light, excessive heat, moisture, and freezing.
Specimen Collection: Consult listed references for appropriate methods for the collection of specimens from environmental and industrial sources.(1-3)
Method of Use: Inoculate the medium as soon as possible after the specimen has been collected. Incubate, with caps loosened, in the appropriate atmospheric environment and incubation temperature for 18-24 hours, or up to five days. Refer to listed references of test protocols and additional procedures using this media.
INTERPRETATION OF RESULTS
Consult listed references for interpretation criteria and further biochemical testing of growth in Tryptic Soy Broth.
Venting of closed containers (vials, bottles, tubes, or jars) during incubation is recommended in order to provide satisfactory growth conditions for aerobes. Use of vented needles is strongly encouraged for serum or septum-capped containers to ensure the growth of obligate aerobes.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.
|Test Organisms||Inoculation Method*||Incubation||Results|
** Tested in accordance with USP <61> and <62>.(6,7)
User Quality Control
Tryptic Soy Broth (TSB), USP should appear clear, and light amber in color.
1. United States Pharmacopoeia and National Formulary (USP-NF). Rockville, MD: United States Pharmacopeial Convention.
2. U.S. Food and Drug Administration. Bacteriological Analytical Manual. Arlington, VA
3. American Public Health Association. Standard Methods for the Examination of Dairy Products, APHA, Washington, D.C.
4. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.
5. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.
6. The Official Compendia of Standards. USP General Chapter <61> Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests. USP-NF. United States Pharmacopeial Convention Inc., Rockville, MD.
7. The Official Compendia of Standards. USP General Chapter <62> Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms. USP-NF. United States Pharmacopeial Convention Inc., Rockville, MD.
ATCC is a registered trademark of the American Type Culture Collection.