TRYPTIC SOY BROTH (TSB), USP

Cat. no. K82 TSB, USP, 16x125mm Tube, 10ml 20 tubes/box
Cat. no. K82BX TSB, USP, 16x125mm Tube, 10ml 80 tubes *ReadyRack
Cat. no. K83 TSB, USP, 20x125mm Tube, 15ml 20 tubes/box
Cat. no. K182 TSB, USP, 16x125mm Tube with Hungate Septum Cap, 10ml 20 tubes/box
Cat. no. K380 TSB, USP, 20x150mm Tube, 20ml 100 tubes/box
Cat. no. K380BX TSB, USP, 20x150mm Tube, 20ml 80 tubes *ReadyRack
Cat. no. U38 TSB, USP, 20ml Serum Vial, 20ml 50 vials/box
Cat. no. U42 TSB, USP, 4oz. Glass Bottle, 100ml 20 bottles/box
Cat. no. U44 TSB, USP, 2oz. Glass Bottle, 50ml 24 bottles/box
Cat. no. U46 TSB, USP, 100ml Serum Bottle, 100ml 20 bottles/box
Cat. no. U56 TSB, USP, 100ml Serum Bottle, 100ml, Unlabeled 20 bottles/box
Cat. no. U62 TSB, USP, 500ml Glass Bottle, 500ml 500ml bottle
Cat. no. U65 TSB, USP, 500ml Polycarbonate Bottle, 500ml 500ml bottle
Cat. no. U67 TSB, USP, 1L Polycarbonate Bottle, 1000ml 10 bottles/box
Cat. no. U71 TSB, USP, 180ml Wide Mouth Jar, 100ml 12 jars/box
Cat. no. U80 TSB, USP, 500ml Wide Mouth Polycarbonate Bottle with Cap, 225ml 10 bottles/box
Cat. no. U82 TSB, USP, 20ml Serum Vial, 15ml 50 vials/box
Cat. no. U100 TSB, USP, 100ml Bag, 100ml 10 bags/box
Cat. no. U133 TSB, USP, 180ml Wide Mouth Jar, 90ml 12 jars/box
Cat. no. U141 TSB, USP, 125ml Polycarbonate Bottle with Septum Cap, 100ml 16 bottles/box
Cat. no. U171 TSB, USP, 250ml Square Polycarbonate Bottle, 160ml 12 bottles/box
Cat. no. U223 TSB, USP, 180ml Wide Mouth Jar, 25ml 12 jars/box
Cat. no. U242 TSB, USP, 16oz. Glass Bottle, 400ml 12 bottles/box
Cat. no. U267 TSB, USP, 100ml Serum Bottle, 50ml 20 bottles/box
Cat. no. U271 TSB, USP, 16oz. Glass Bottle with Septum Cap, 400ml 12 bottles/box
Cat. no. U274 TSB, USP, 500ml Glass Bottle, 500ml 10 bottles/box
Cat. no. U316 TSB, USP, 8oz. Wide Mouth Jar, 180ml 12 jars/box
Cat. no. U421 TSB, USP, 500ml Glass Bottle with Septum Cap, 300ml 10 bottles/box
Cat. no. U435 TSB, USP, 8oz. Glass Bottle, 200ml 12 bottles/box
Cat. no. U453 TSB, USP, 300ml Polycarbonate Bottle with Cap, 90ml 10 bottles/box
Cat. no. U457 TSB, USP, 290ml Polycarbonate Bottle with Cap, 290ml 10 bottles/box
Cat. no. U8210 TSB, USP, 20ml Serum Vial, 10ml 50 vials/box

*ReadyRack is a plastic rack designed for cleanroom use and can be rinsed with alcohol.

INTENDED USE

Hardy Diagnostics Tryptic Soy Broth is recommended for use as a general purpose medium for the isolation and cultivation of a wide variety of bacteria and fungi. The U.S. Pharmacopeia National Formulary (USP) describes its use for sterility testing.(1)

This product is not intended to be used for the diagnosis of human disease.

SUMMARY

Tryptic Soy Broth is widely used for the cultivation of microorganisms from environmental sources; supporting the growth of the majority of bacteria and fungi. Tubes of this medium may be used for preparing dilutions of organism for colony counts and preparation of standard inocula. Tryptic Soy Broth is also recommended for use in sterility testing for the detection of contamination with low incidence fungi and aerobic bacteria.(1)

Tryptic Soy Broth, also known as Soybean-Casein Digest, conforms to the formula given by the U.S. Pharmacopeia.(1) This medium contains digests of soybean meal and casein, which provide amino acids and other nitrogenous substances, making it a highly nutritious medium for a variety of organisms. Sodium chloride is added to maintain the osmotic equilibrium. Dextrose is incorporated as an energy source. The dipotassium phosphate is included in the formulation as a buffer to maintain the pH.

FORMULA

Ingredients per liter of deionized water:*

Pancreatic Digest of Casein 17.0gm
Sodium Chloride 5.0gm
Papaic Digest of Soybean Meal 3.0gm
Dextrose 2.5gm
Dipotassium Phosphate 2.5gm

Final pH 7.3 +/- 0.2 at 25°C.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-25°C. away from direct light. Media should not be used if there are any signs of deterioration (discoloration), contamination, or if the expiration date has passed. Protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

PROCEDURE

Specimen Collection: Consult listed references for appropriate methods for the collection of specimens from environmental and industrial sources.(1-3)

Method of Use: Inoculate the medium as soon as possible after the specimen has been collected. Incubate, with caps loosened, in the appropriate atmospheric environment and incubation temperature for 18-24 hours, or up to five days. Refer to listed references of test protocols and additional procedures using this media.

INTERPRETATION OF RESULTS

Consult listed references for interpretation criteria and further biochemical testing of growth in Tryptic Soy Broth.

LIMITATIONS

Venting of closed containers (vials, bottles, tubes, or jars) during incubation is recommended in order to provide satisfactory growth conditions for aerobes. Use of vented needles is strongly encouraged for serum or septum-capped containers to ensure the growth of obligate aerobes.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Staphylococcus aureus
ATCC® 6538**
J 24-72 hrs 30-35°C Aerobic Growth
Pseudomonas aeruginosa
ATCC® 9027**
J 24-72 hrs 30-35°C Aerobic Growth
Bacillus subtilis
ATCC® 6633**
J 24-72 hrs 30-35°C Aerobic Growth
Bacillus subtilis
ATCC® 6633**
J 24-72 hrs 20-25°C Aerobic Growth
Candida albicans
ATCC® 10231**
J 3-5 days 20-25°C Aerobic Growth
Aspergillus brasiliensis
ATCC® 16404**
J 5 days 20-25°C Aerobic Growth

** Tested in accordance with USP <61> and <62>.(6,7)

User Quality Control

PHYSICAL APPEARANCE

Tryptic Soy Broth (TSB), USP should appear clear, and light amber in color.

REFERENCES

1. United States Pharmacopoeia and National Formulary (USP-NF). Rockville, MD: United States Pharmacopeial Convention.

2. U.S. Food and Drug Administration. Bacteriological Analytical Manual. Arlington, VA
http://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm2006949.htm

3. American Public Health Association. Standard Methods for the Examination of Dairy Products, APHA, Washington, D.C.

4. APHA Technical Committee on Microbiological Methods for Foods. Compendium of Methods for the Microbiological Examination of Foods, APHA, Washington, D.C.

5. American Public Health Association. Standard Methods for the Examination of Water and Wastewater, APHA, Washington, D.C.

6. The Official Compendia of Standards. USP General Chapter <61> Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests. USP-NF. United States Pharmacopeial Convention Inc., Rockville, MD.

7. The Official Compendia of Standards. USP General Chapter <62> Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms. USP-NF. United States Pharmacopeial Convention Inc., Rockville, MD.

ATCC is a registered trademark of the American Type Culture Collection.

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