UriStain™

Cat. no. Z74 UriStain™, Urine Sediment Stain 15ml

INTENDED USE

Hardy Diagnostics UriStain™ is intended for use in sediment staining of urine specimens.

SUMMARY

The stain contains various dyes that aid in differentiating the abnormal and normal cellular elements found in urine. UriStain™ is a one solution modification of the Sternheimer and Malvin procedure.

REAGENT FORMULA

CAS No.
Ammonium Oxalate 113-38-8
Safranin 477-73-6
Crystal Violet 548-62-9
Ethanol 64-17-5
Deionized Water

This stain is made from certified dyes.

STORAGE AND SHELF LIFE

Upon receipt store at 2-30ºC. Product should not be used if there are any signs of deterioration or if the expiration date has past.

PRECAUTIONS

Warning! This product is poisonous and may be fatal or cause blindness if ingested.

Warning! This solution is an irritant. Vapor of ethanol is harmful.

Warning! This product is flammable; keep away from heat, sparks, or flames.

PROCEDURE

Specimen Collection: A fresh, clean-voided urine specimen should be used. When microscopic findings are not normal or a culture is required, the patient must be cleansed before voiding.

Method of Use:

1. Mix the specimen well, as casts tend to settle out.

2. Pour 10-12ml of the sample into a conical tube or test tube and centrifuge at 2000rpm for five minutes.

3. Remove supernatant, leaving a small amount of urine in the tube (1ml).

4. Suspend the sediment. One or two drops of the unstained sediment should be examined with the stained sediment. Place one to two drops of UriStain™ in the remaining sediment and mix.

5. On a clean slide, place one drop of stained sediment and one drop of unstained sediment. Cover each drop with a coverslip, avoiding bubbles.

6. Examine with low power and subdued light, and scan the entire area. Casts will be found along the edges of the coverslip. They are counted under low power (100X) and differentiated under high-dry power (400X). Red blood cells, leukocytes, and epithelial cells are counted in ten fields. Large numbers of squamous epithelial cells, if present, are noted. Bacteria and yeasts are also reported. If crystals are present in large amounts, they are reported.

INTERPRETATION OF RESULTS

Cell Type Reaction
Erythrocytes Faint pink color
Yeast cells Purple
Epithelial cells Nuclei stain deep purple
Cytoplasm Stains a red/purple color
Leukocytes Granulocyte(s) of nuclei stain dark purple
Hyaline casts Stain pink to red
Granular elements Stain red to violet
Fat droplets Brilliant, refractive honeycomb structure
Bacteria Dead: stain dark purple; Alive: colorless to red
Fungi mycelial and spores Appear light purple
Trichomonas Either colorless or light blue

LIMITATIONS

The presence of bacteria may be due to non-sterile conditions of the patient and collection container.

Air bubbles under the coverslip may be confused with fat droplets or red blood cells.

Slide and coverslip must be clean and free of lint and oils. If precipitate is found on the slide, filter the stain and perform the staining procedure again before reporting.

If the sample cannot be tested immediately, it should be refrigerated or a preservative added.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as slides, coverslips, centrifuge, centrifuge tubes, microscopes and specimen cups, etc., are not provided.

User Quality Control

It is recommended that each new lot and each shipment of reagent be tested with known positive and negative controls and retested each week of use thereafter.(1)

It is recommended that positive controls be run in parallel with patient specimens and that results from any staining procedure be reported only if positive control smears are acceptable.

The microscope should be calibrated (within the last 12 months). The objectives and oculars used for the calibration procedure should be in place on the microscope when objects are measured.(4)

Physical Appearance

UriStain™ should appear opaque and bright blue-violet to purple in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Levinson, S.A. and R.P. MacFate. Clinical Laboratory Diagnosis.

3. Todd, J.C., and A.H. Stanford. Clinical Diagnosis by Laboratory Methods.


080816gr