|Cat. no. Z74||UriStain™, Urine Sediment Stain||15ml|
Hardy Diagnostics UriStain™ is intended for use in sediment staining of urine specimens.
The stain contains various dyes that aid in differentiating the abnormal and normal cellular elements found in urine. UriStain™ is a one solution modification of the Sternheimer and Malvin procedure.
This stain is made from certified dyes.
STORAGE AND SHELF LIFE
Upon receipt store at 2-30ºC. Product should not be used if there are any signs of deterioration or if the expiration date has past.
Warning! This product is poisonous and may be fatal or cause blindness if ingested.
Warning! This solution is an irritant. Vapor of ethanol is harmful.
Warning! This product is flammable; keep away from heat, sparks, or flames.
Specimen Collection: A fresh, clean-voided urine specimen should be used. When microscopic findings are not normal or a culture is required, the patient must be cleansed before voiding.
Method of Use:
1. Mix the specimen well, as casts tend to settle out.
2. Pour 10-12ml of the sample into a conical tube or test tube and centrifuge at 2000rpm for five minutes.
3. Remove supernatant, leaving a small amount of urine in the tube (1ml).
4. Suspend the sediment. One or two drops of the unstained sediment should be examined with the stained sediment. Place one to two drops of UriStain™ in the remaining sediment and mix.
5. On a clean slide, place one drop of stained sediment and one drop of unstained sediment. Cover each drop with a coverslip, avoiding bubbles.
6. Examine with low power and subdued light, and scan the entire area. Casts will be found along the edges of the coverslip. They are counted under low power (100X) and differentiated under high-dry power (400X). Red blood cells, leukocytes, and epithelial cells are counted in ten fields. Large numbers of squamous epithelial cells, if present, are noted. Bacteria and yeasts are also reported. If crystals are present in large amounts, they are reported.
INTERPRETATION OF RESULTS
|Erythrocytes||Faint pink color|
|Epithelial cells||Nuclei stain deep purple|
|Cytoplasm||Stains a red/purple color|
|Leukocytes||Granulocyte(s) of nuclei stain dark purple|
|Hyaline casts||Stain pink to red|
|Granular elements||Stain red to violet|
|Fat droplets||Brilliant, refractive honeycomb structure|
|Bacteria||Dead: stain dark purple; Alive: colorless to red|
|Fungi mycelial and spores||Appear light purple|
|Trichomonas||Either colorless or light blue|
The presence of bacteria may be due to non-sterile conditions of the patient and collection container.
Air bubbles under the coverslip may be confused with fat droplets or red blood cells.
Slide and coverslip must be clean and free of lint and oils. If precipitate is found on the slide, filter the stain and perform the staining procedure again before reporting.
If the sample cannot be tested immediately, it should be refrigerated or a preservative added.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as slides, coverslips, centrifuge, centrifuge tubes, microscopes and specimen cups, etc., are not provided.
User Quality Control
It is recommended that each new lot and each shipment of reagent be tested with known positive and negative controls and retested each week of use thereafter.(1)
It is recommended that positive controls be run in parallel with patient specimens and that results from any staining procedure be reported only if positive control smears are acceptable.
The microscope should be calibrated (within the last 12 months). The objectives and oculars used for the calibration procedure should be in place on the microscope when objects are measured.(4)
UriStain™ should appear opaque and bright blue-violet to purple in color.
1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.
2. Levinson, S.A. and R.P. MacFate. Clinical Laboratory Diagnosis.
3. Todd, J.C., and A.H. Stanford. Clinical Diagnosis by Laboratory Methods.