|Cat. no. G98||V9 Agar, 15x100mm Plate, 22ml||10 plates/bag|
Hardy Diagnostics V9 Agar is recommended for the isolation, cultivation and rapid sporulation of fungi. The plates are deep-filled to reduce the effects of drying during prolonged incubation.
This product is not intended to be used for the diagnosis of human disease.
Developed in 1965 as part of a research project, V9 Agar was noted to induce early sporulation in environmentally isolated yeasts and molds when compared with peptone and sugar based formulas. The primary ingredients of the media are V8™ Juice and potatoes, thus the name V9 Agar. (5) The naturally low pH makes the media inhibitory to most bacteria.
Ingredients per 650ml of deionized water:*
|Dehydrated Potato Flakes||20.0gm|
Final pH 4.5 +/- 0.3 at 25ºC.
* Adjusted and/or supplemented as required to meet performance criteria.
STORAGE AND SHELF LIFE
Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.
Specimen Collection: Infectious material should be submitted directly to the laboratory in a sterile container, or other appropriate means of transport. Consult appropriate references for specimen collection and transport. (1-8) Samples should be processed as soon as possible upon arrival in the laboratory. Observe biohazard precautions when handling the specimen.
Method of Use: Prior to inoculation, media should be brought to room temperature. Cutaneous specimens should be lightly imbedded in the agar. Larger specimens should be macerated with sterile normal saline in a tissue grinder, then inoculated onto the medium. Liquid specimens may be streaked directly onto the agar surface using the four quadrant technique to obtain isolated colonies. Incubate 25-30ºC. in an inverted position (agar side up). All cultures should be examined at least weekly for fungal growth and should be incubated 4-6 weeks before being reported out as negative.
INTERPRETATION OF RESULTS
After sufficient incubation, examine fungal cultures microscopically for characteristic mycelia and fruiting structures. Consult appropriate references for fungal identification. (1-8) Biochemical and serological tests should be performed on pure cultures for complete identification.
Colony morphology on this media may not be sufficient for the identification of fungi. Further biochemical, physiological, serological tests and microscopic morphology of pure cultures are recommended for complete identification.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.
|Test Organisms||Inoculation Method*||Incubation||Results|
ATCC ® 9533
ATCC ® 28188
User Quality Control
V9 Agar should appear opaque, granular, and orange in color.
Trichophyton mentagrophytes (ATCC ® 9533) growing on V9 Agar (Cat. no. G98). Incubated aerobically for 7 days at 30ºC.
Uninoculated plate of V9 Agar (Cat. no. G98).
1. Jorgensen., et al. Manual of Clinical Microbiology , American Society for Microbiology, Washington, D.C.
2. August, M.J., et al. 1990. Cumitech 3A; Quality Control and Quality Assurance Practices in Clinical Microbiology , Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.
3. Koneman, E.W., et al. 1997. Color Atlas and Textbook of Diagnostic Microbiology , 5th ed. J.B. Lippincott Company, Philadelphia, PA.
4. Kwon-Chung, K.J. and J.E. Bennett. 1992. Medical Mycology . Lea and Febiger, Malvern, PA.
5. Dillavou, C.L. 1978. V9 Agar: A new medium for medical mycology. Letterman Army Medical Center, San Francisco, CA, unpublished paper.
6. Isenberg, H.D. Clinical Microbiology Procedures Handbook , Vol. I & II. American Society for Microbiology, Washington, D.C.
7. Larone, D.H. 1993. Medically Important Fungi: A Guide to Identification , 2nd ed. American Society for Microbiology, Washington, D.C.
8. St. Germain, Guy, et al. 1996. Identifying Filamentous Fungi . Star Publishing Company, Belmont, CA.
ATCC is a registered trademark of the American Type Culture Collection.
V8 is a trademark of Campbell Soup Company, Camden, NJ.