V9 AGAR

Cat. no. G98 V9 Agar, 15x100mm Plate, 22ml 10 plates/bag

INTENDED USE

Hardy Diagnostics V9 Agar is recommended for the isolation, cultivation and rapid sporulation of fungi. The plates are deep-filled to reduce the effects of drying during prolonged incubation.

This product is not intended to be used for the diagnosis of human disease.

SUMMARY

Developed in 1965 as part of a research project, V9 Agar was noted to induce early sporulation in environmentally isolated yeasts and molds when compared with peptone and sugar based formulas. The primary ingredients of the media are V8™ Juice and potatoes, thus the name V9 Agar. (5) The naturally low pH makes the media inhibitory to most bacteria.

FORMULA

Ingredients per 650ml of deionized water:*

Dehydrated Potato Flakes 20.0gm
V8™ Juice 350.0ml
Agar 20.0gm

Final pH 4.5 +/- 0.3 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

PROCEDURE

Specimen Collection: Infectious material should be submitted directly to the laboratory in a sterile container, or other appropriate means of transport. Consult appropriate references for specimen collection and transport. (1-8) Samples should be processed as soon as possible upon arrival in the laboratory. Observe biohazard precautions when handling the specimen.

Method of Use: Prior to inoculation, media should be brought to room temperature. Cutaneous specimens should be lightly imbedded in the agar. Larger specimens should be macerated with sterile normal saline in a tissue grinder, then inoculated onto the medium. Liquid specimens may be streaked directly onto the agar surface using the four quadrant technique to obtain isolated colonies. Incubate 25-30ºC. in an inverted position (agar side up). All cultures should be examined at least weekly for fungal growth and should be incubated 4-6 weeks before being reported out as negative.

INTERPRETATION OF RESULTS

After sufficient incubation, examine fungal cultures microscopically for characteristic mycelia and fruiting structures. Consult appropriate references for fungal identification. (1-8) Biochemical and serological tests should be performed on pure cultures for complete identification.

LIMITATIONS

Colony morphology on this media may not be sufficient for the identification of fungi. Further biochemical, physiological, serological tests and microscopic morphology of pure cultures are recommended for complete identification.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, swabs, applicator sticks, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Trichophyton mentagrophytes
ATCC ® 9533
A 7 days 15-30°C Aerobic Growth
Trichophyton rubrum
ATCC ® 28188
A 7 days 15-30°C Aerobic Growth

User Quality Control

PHYSICAL APPEARANCE

V9 Agar should appear opaque, granular, and orange in color.

T. mentagrophytes growing on V9 Agar

Trichophyton mentagrophytes (ATCC ® 9533) growing on V9 Agar (Cat. no. G98). Incubated aerobically for 7 days at 30ºC.

V9 Agar

Uninoculated plate of V9 Agar (Cat. no. G98).

REFERENCES

1. Jorgensen., et al. Manual of Clinical Microbiology , American Society for Microbiology, Washington, D.C.

2. August, M.J., et al. 1990. Cumitech 3A; Quality Control and Quality Assurance Practices in Clinical Microbiology , Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

3. Koneman, E.W., et al. 1997. Color Atlas and Textbook of Diagnostic Microbiology , 5th ed. J.B. Lippincott Company, Philadelphia, PA.

4. Kwon-Chung, K.J. and J.E. Bennett. 1992. Medical Mycology . Lea and Febiger, Malvern, PA.

5. Dillavou, C.L. 1978. V9 Agar: A new medium for medical mycology. Letterman Army Medical Center, San Francisco, CA, unpublished paper.

6. Isenberg, H.D. Clinical Microbiology Procedures Handbook , Vol. I & II. American Society for Microbiology, Washington, D.C.

7. Larone, D.H. 1993. Medically Important Fungi: A Guide to Identification , 2nd ed. American Society for Microbiology, Washington, D.C.

8. St. Germain, Guy, et al. 1996. Identifying Filamentous Fungi . Star Publishing Company, Belmont, CA.


ATCC is a registered trademark of the American Type Culture Collection.
V8 is a trademark of Campbell Soup Company, Camden, NJ.

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