VEILLONELLA

15 plus species including:

SPECIES

MICROSCOPIC APPEARANCE

Gram Stain: Gram-negative, but tend to resist staining.
Morphology: Spherical, kidney shaped, or cocci cells; appearing by light microscopy as diplococci. Occurring in masses and short chains.
Size: 0.3 micrometers to 0.5 micrometers in diameter.
Motility: Non-motile.
Capsules: None.
Spores: None.
Other: Non-hemolytic on Blood Agar.

MACROSCOPIC APPEARANCE

Colonies in Lactate Agar Media appear diamond or heart shaped, smooth, opaque, grayish-white and butyrous.

KEY BIOCHEMICAL REACTIONS

METABOLIC PROPERTIES

Anaerobic. Chemoorganotrophic, fermentative type metabolism.
Carbohydrates not fermented. CO 2 is required for growth; nutritional requirements are complex.

HABITAT

Parasites in the mouth, intestinal and respiratory tracts of man and other animals.

PATHOGENICITY

Veillonella is seldom a significant pathogen. These organisms produce serologically specific endotoxins (lipopolysaccharides) which induce pyrogenicity and the Schwarzman phenomenon in rabbits.

RECOMMENDED MEDIA

For culture: Brain Heart Infusion (BHI) Agar or Lactate Agar Media.
For selective isolation: Lactate Agar Media with 715mcg/ml Vancomycin.
For maintenance: Lyophilization in Skim Milk for long-term storage.

INCUBATION

Temperature: 30-37 degrees C.
Time: 48 hours.
Atmosphere: Anaerobic.
pH: 6.5-8.0.

REFERENCES

1. Holt, J.G., et al. 1994. Bergey's Manual of Determinative Bacteriology , 9th ed. Williams & Wilkins, Baltimore, MD.

2. Holt, J.G., et al. 1986. Bergey's Manual of Systemic Bacteriology , Vol. I & II. Williams & Wilkins, Baltimore, MD.

3. The Oxoid Vade-Mecum of Microbiology . 1993. Unipath Ltd., Basingstoke, UK.

4. Murray, P.R., et al. 1995. Manual of Clinical Microbiology , 6th ed. American Society for Microbiology, Washington, D.C.

5. Internet: www.hardlink.com /Bacterial Database Search, February, 1998.

6. Hensyl, B.R., et al. 1990. Stedman's Medical Dictionary , 25th ed. Williams & Wilkins, Baltimore, MD.

7. Koneman, et al. 1997. Color Atlas and Textbook of Diagnostic Microbiology , 5th ed. Lippincott, Philadelphia, PA.

8. Howard, B.J., et al. 1994. Clinical and Pathogenic Microbiology , 2nd ed. Mosby, St. Louis, IL.


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