VIRAL TRANSPORT MEDIA
|Cat. no. R99||Viral Transport, 15x120mm Tube, 2ml||20 or 100 tubes/box|
Viral Transport Media is provided in plastic centrifuge tubes and is used for the collection and transport of clinical specimens for the recovery of viral agents including; Herpes Simplex Type I, Herpes Simplex Type II, Cytomegalovirus (CMV), Influenzae A, Influenzae B, Respiratory Syncytial Virus (RSV), Echovirus, Adenovirus.
Viral Transport consists of Modified Hank's Balanced Salt Solution supplemented with bovine serum albumin, sucrose, glutamic acid, and gelatin. A buffer is used to maintain a pH 7.3 +/- 0.2. Phenol red is used as a pH indicator. Amphotericin B (4ug/ml), colistin (7.5ug/ml), and vancomycin (100ug/ml) are added to inhibit contaminants. The media contains cryoprotectorants to ensure viability of organisms through freezing and thawing.
STORAGE AND SHELF LIFE
Upon receipt store at 2-8ºC. Products should not be used if there are any signs of deterioration, leakage, pH change, or contamination, or if the expiration date has passed.
Aseptically collect sample. Use swabs and shafts that are non-toxic to viral agents, such as Dacron swabs. Do not use cotton or calcium alginate swabs for collection of specimens.
After collection of specimen, remove cap from tube, insert swab. Submerge the tip of the swab into the media. Break off swab shaft by bending the shaft against the rim of the tube. Recap the tube securely. Label the sample. Transport immediately to the lab at 2-30ºC. within 48 hours. For long-term storage, freeze at -70ºC. Do not freeze at temperatures greater than -70ºC. For more information, consult listed references.(2-5)
More than one tube may be submitted if more than one type of culture is requested to ensure adequate specimen for culture set-ups.
All specimens should be shipped in compliance with all federal, state and hospital guidelines.
INTERPRETATION OF RESULTS
Consult listed references for isolation and identification procedures for specific organisms recovered from transport media.(2-5)
Inoculate specimens as soon after collection as possible. If there is to be a delay in inoculating, store at 2-30 degrees C. For long term storage of specimens for the recovery of viruses, freeze at -70ºC.
Repeated freezing and thawing of frozen specimens may reduce the recovery of viable organisms.
Dacron, or rayon, tipped swabs are recommended. Calcium alginate or cotton swabs, as well as wooden stick swabs, should not be used.
Data represented in the Performance Characteristics section is for culture only. Performance has not been established for EIA testing. It is recommended that each laboratory establish performance characteristics of this product in conjunction with its viral testing methods.
MATERIALS REQUIRED BUT NOT PROVIDED
Standard microbiological supplies and equipment such as loops, incinerators, incubators, centrifuge, pasteur pipets, cell culture media, etc., as well as serological and biochemical reagents, are not provided.
After inoculation to the Viral Transport Media, the organisms listed below are held at 2-8ºC., for the indicated time, and then subcultured to appropriate culture media.
|Herpes Simplex Type I McIntyre
Toxicity: A 0.3ml aliquot of the Viral Transport is inoculated into MRC-5 shell vials. The vials are incubated for 18-24 hours at 35ºC. The result is recorded as normal or abnormal as determined by visual determination of the cells under a microscope.
User Quality Control
Viral Transport Media should appear clear, and light peach in color.
Viral Transport was compared to a commercially available multimicrobe transport media. Both transports are intended for use in the transport and maintenance of specimens for the recovery of viral agents. The results for the eight organisms (wild-type strains) tested in Viral Transport were as follows:
(Percent of recovery was not determined)
|Herpes Simplex Type I||10-3 TCID50||96 hours||96 hours|
|Herpes Simplex Type II||10-3 TCID50||96 hours||96 hours|
|Cytomegalovirus||n/t||72 hours||24 hours|
|Influenzae A||10-1 TCID50||72 hours||72 hours|
|Influenzae B||10-1 TCID50||72 hours||48 hours|
|RSV||10-1 TCID50||72 hours||72 hours|
|Echovirus||10-1 TCID50||96 hours||96 hours|
|Adenovirus||10-1 TCID50||96 hours||72 hours|
1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.
2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.
3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.
4. Clyde, W.A., et al. 1984. Cumitech 19; Laboratory Diagnosis of Chlamydial and Mycoplasmal Infections, Coordinating ed., W.L. Drew. American Society for Microbiology, Washington D.C.
5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.
ATCC is a registered trademark of the American Type Culture Collection.