VIRAL TRANSPORT MEDIA

Cat. no. R99 Viral Transport, 15x120mm Tube, 2ml 20 or 100 tubes/box
Cat. no. R99K Viral Transport Kit, 15x120mm Tube with
Dacron Swab in a Zip-lock Bag, 2ml
100 kits/box

Viral Transport Media Kits include 1 tube of transport media, a sterile dacron swab on a scored plastic shaft in a zip-lock bag; packed 100 kits per box.

INTENDED USE

Viral Transport Media is provided in plastic centrifuge tubes and is used for the collection and transport of clinical specimens for the recovery of viral agents including; Herpes Simplex Type I, Herpes Simplex Type II, Cytomegalovirus (CMV), Influenzae A, Influenzae B, Respiratory Syncytial Virus (RSV), Echovirus, Adenovirus.

FORMULA

Viral Transport consists of Modified Hank's Balanced Salt Solution supplemented with bovine serum albumin, sucrose, glutamic acid, and gelatin. A buffer is used to maintain a pH 7.3 +/- 0.2. Phenol red is used as a pH indicator. Amphotericin B (4ug/ml), colistin (7.5ug/ml), and vancomycin (100ug/ml) are added to inhibit contaminants. The media contains cryoprotectorants to ensure viability of organisms through freezing and thawing.

STORAGE AND SHELF LIFE

Upon receipt store at 2-8ºC. Products should not be used if there are any signs of deterioration, leakage, pH change, or contamination, or if the expiration date has passed.

PRECAUTIONS

PROCEDURE

Aseptically collect sample. Use swabs and shafts that are non-toxic to viral agents, such as Dacron swabs. Do not use cotton or calcium alginate swabs for collection of specimens.

After collection of specimen, remove cap from tube, insert swab. Submerge the tip of the swab into the media. Break off swab shaft by bending the shaft against the rim of the tube. Recap the tube securely. Label the sample. Transport immediately to the lab at 2-30ºC. within 48 hours. For long-term storage, freeze at -70ºC. Do not freeze at temperatures greater than -70ºC. For more information, consult listed references. (2-5)

More than one tube may be submitted if more than one type of culture is requested to ensure adequate specimen for culture set-ups.

All specimens should be shipped in compliance with all federal, state and hospital guidelines.

INTERPRETATION OF RESULTS

Consult listed references for isolation and identification procedures for specific organisms recovered from transport media. (2-5)

LIMITATIONS

Inoculate specimens as soon after collection as possible. If there is to be a delay in inoculating, store at 2-30 degrees C. For long term storage of specimens for the recovery of viruses, freeze at -70ºC.

Repeated freezing and thawing of frozen specimens may reduce the recovery of viable organisms.

Dacron, or rayon, tipped swabs are recommended. Calcium alginate or cotton swabs, as well as wooden stick swabs, should not be used.

Data represented in the Performance Characteristics section is for culture only. Performance has not been established for EIA testing. It is recommended that each laboratory establish performance characteristics of this product in conjunction with its viral testing methods.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, incinerators, incubators, centrifuge, pasteur pipets, cell culture media, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

After inoculation to the Viral Transport Media, the organisms listed below are held at 2-8ºC., for the indicated time, and then subcultured to appropriate culture media.

Test Organisms Time Reaction
Herpes Simplex Type I McIntyre

ATCC ® VR-539
48hr
Viability maintained

Toxicity: A 0.3ml aliquot of the Viral Transport is inoculated into MRC-5 shell vials. The vials are incubated for 18-24 hours at 35ºC. The result is recorded as normal or abnormal as determined by visual determination of the cells under a microscope.

User Quality Control

Physical Appearance

Viral Transport Media should appear clear, and light peach in color.

PERFORMANCE CHARACTERISTICS

Viral Transport was compared to a commercially available multimicrobe transport media. Both transports are intended for use in the transport and maintenance of specimens for the recovery of viral agents. The results for the eight organisms (wild-type strains) tested in Viral Transport were as follows:

Organism
(Wild-type strains:
One isolate/organism)

Inoculum
Concentration
Maximum Holding Time
(Percent of recovery was not determined)
2-8°C. 15-30°C.
Herpes Simplex Type I 10 -3 TCID50 96 hours 96 hours
Herpes Simplex Type II 10 -3 TCID50 96 hours 96 hours
Cytomegalovirus n/t 72 hours 24 hours
Influenzae A 10 -1 TCID50 72 hours 72 hours
Influenzae B 10 -1 TCID50 72 hours 48 hours
RSV 10 -1 TCID50 72 hours 72 hours
Echovirus 10 -1 TCID50 96 hours 96 hours
Adenovirus 10 -1 TCID50 96 hours 72 hours

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Clyde, W.A., et al. 1984. Cumitech 19; Laboratory Diagnosis of Chlamydial and Mycoplasmal Infections, Coordinating ed., W.L. Drew. American Society for Microbiology, Washington D.C.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.


ATCC is a registered trademark of the American Type Culture Collection.

041816gr