YM-11 Agar

Cat. no. G01 YM-11 Agar, 15x100mm Plate, 18ml 10 plates/bag

INTENDED USE

Hardy Diagnostics YM-11 Agar is recommended for the isolation of yeast and molds.

SUMMARY

YM-11 agar is prepared according to the formula published by Wickerham, which suggested that the selectivity of a medium may be enhanced through acidification or addition of selective agents. (7) YM-11 agar contains a rich source of trace elements, vitamins, amino acids, and carbon sources to provide optimum growing conditions for yeast and molds.

Yeast and molds are chemoorganotrophs which consume organic compounds as their primary energy source, and do not require sunlight to grow. Their primary carbon source is obtained from hexose sugars such as dextrose, glucose, and fructose. Yeast and malt extracts are added to provide additional nutrients needed to enhance growth. Gelatin peptone is added to provide a rich source of protein, and selective agents are added to inhibit bacteria.

FORMULA

Ingredients per liter of deionized water:*

Dextrose 10.0gm
Gelatin Peptone 5.0gm
Malt Extract 3.0gm
Yeast Extract 3.0gm
Selective Agents 0.1gm
Agar 20.0gm

Final pH 7.0 +/- 0.3 at 25ºC.

* Adjusted and/or supplemented as required to meet performance criteria.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-8ºC. away from direct light. Media should not be used if there are any signs of deterioration (shrinking, cracking, or discoloration), contamination, or if the expiration date has passed. Product is light and temperature sensitive; protect from light, excessive heat, moisture, and freezing.

PRECAUTIONS

PROCEDURE

Inoculate YM-11 Agar plates with sample to evaluate the presence of yeast and molds. Incubate plates aerobically at 15-30ºC. for up to 7 days until growth is observed.

INTERPRETATION OF RESULTS

Record YM-11 Agar results as colony forming units (CFU) per volume of sample.

LIMITATIONS

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, swabs, applicator sticks, other culture media, incinerators, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Inoculation Method* Incubation Results
Time Temperature Atmosphere
Trichophyton mentagrophytes
ATCC ® 9533**
A 7 days 15-30°C Aerobic Growth
Candida albicans
ATCC ® 10231**
A 7 days 15-30°C Aerobic Growth

** Recommended QC strains for User Quality Control according to the CLSI document M22 when applicable.

USER QUALITY CONTROL

Physical Appearance

Hardy Diagnostics YM-11 Agar should appear clear, and light bluish-gray in color.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.

5. Koneman, E.W., et al. 2006. Color Atlas and Textbook of Diagnostic Microbiology, 6th ed. J.B. Lippincott Company, Philadelphia, PA.

6. Quality Assurance for Commercially Prepared Microbiological Culture Media , M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.

7. Wickerham. 1939. J. Tropical Med. Hyg. ; 42:176.


ATCC is a registered trademark of the American Type Culture Collection.

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