ANAEROGEN™ COMPACT

Cat. no. AN010C AnaeroGen™ Compact 10 sachets/box

INTENDED USE

AnaeroGen™ Compact is a simple system for the anaerobic incubation of up to four petri dishes or an identification panel.

SUMMARY

The system consists of a plastic pouch and a paper gas generating sachet. The paper sachet contains ascorbic acid and is activated carbon which react on contact with air. Oxygen is rapidly absorbed and carbon dioxide is produced. When the paper sachet is placed in a sealed plastic pouch, this reaction will create ideal atmospheric conditions for the growth of anerobes. It proceeds with no evolution of hydrogen, and therefore does not require a catalyst. No addition of water is necessary to activate the reaction. This gives the system many advantages over the commonly used borohydride systems with increased safety and convenience.

When used as directed the AnaeroGen™ Compact sachet will reduce the oxygen content in the pouch to below 1% within 30 minutes. The resulting carbon dioxide content will be between 8% and 14%. The level of carbon dioxide will depend on how many plates are placed in the pouch. AnaeroGen™ Compact has been designed for use with 1-4 plates.

FORMULA

Ascorbic acid is the reactive component within each paper sachet.

STORAGE AND SHELF LIFE

Store at 2-25ºC. Under these conditions, the AnaeroGen™ Compact sachets will retain their activity until the expiration date declared on the outer box and on the foil wrapped sachet. Products should not be used if there are any signs of deterioration (tears), or if the expiration date has passed. Protect from excessive heat and freezing.

PRECAUTIONS

PROCEDURE

Method of Use:

1. Place the inoculated media plates or identification panel in the plastic pouch provided. Disposable plastic petri dishes should be of the vented variety to aid gas transfer between the interior and exterior of the plates.

2. Tear open an AnaeroGen™ Compact foil sachet at the tear-neck indicated. Remove the AnaeroGen™ Compact paper sachet from within.

3. Immediately place the AnaeroGen™ Compact paper sachet in the plastic pouch.

Note: The AnaeroGen™ Compact paper sachet will become warm to the touch on exposure to air.

4. Expel excess air from the plastic pouch. Seal the plastic pouch immediately with the AnaeroGen™ Compact Clip (Cat. no. AN005C) or equivalent.

Note: The time taken between opening the foil sachet and sealing the plastic pouch should not exceed 1 minute. Extended exposure will result in loss of reactivity, and full anaerobic conditions may not be achieved in the pouch.

5. Incubate appropriately.

6. After the incubation period remove the plates or ID panel and examine for the presence of colonies or biochemical reaction. If the plates require re-incubation then a fresh AnaeroGen™ Compact sachet must be used following steps 2-5 described above.

Note: The plates may be initially inspected through the transparent plastic pouch. If the bag is not opened, a fresh AnaeroGen™ Compact sachet is not required for re-incubation.

7. After incubation, the exhausted AnaeroGen™ Compact paper sachet and plastic pouch should be sterilized and discarded with the non-hazardous laboratory waste.

LIMITATIONS

It is essential that the gas generating paper sachet be placed and sealed in the jar within one minute of its removal from the outer foil sachet. Extended exposure to air will result in loss of reactivity and full micraephilic/anaerobic conditions may not be achieved.

MATERIALS REQUIRED BUT NOT PROVIDED

AnaeroGen™ Compact Sealing Clips (Cat. no. AN005C)

Standard microbiological supplies and equipment such as loops, slides, staining supplies, other culture media, microscope, incinerator, incubators, seal bars or clips, as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Reaction
Clostridium novyii
ATCC® 9690
Growth
Micrococcus luteus
ATCC® 9341
Growth

PHYSICAL APPEARANCE

AnaeroGen™ Compact should appear as white paper sachets individually packaged within a foil pouch.

AnaeroGen Compact

AnaeroGen™ Compact (Cat. no. AN010C)

REFERENCES

1.Van Horn, K.G., et al. 1997. Journal of Clinical Microbiology, Vol. 35, No. 8, p. 2170-2173, American Society for Microbiology.

2. August, M.J., et al. 1990. Cumitech 3A; Quality Control and Quality Assurance Practices in Clinical Microbiology, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

3. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.

4. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.

5. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I & II. American Society for Microbiology, Washington, D.C.

6. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.

7. Package Insert, Oxoid, Unipath Ltd., Wade Road, Basingtoke, Hampshire, RG24 8PW, England. March, 1997.

ATCC is a registered trademark of the American Type Culture Collection.
AnaeroGen is a trademark of Oxoid.

IFU-10034[A]