CAMPYGEN™ AND ANAEROGEN™ GAS GENERATING SYSTEMS

Cat. no. AN25US AnaeroGen™ (for 2.5L jar) 10 sachets/box
Cat. no. AN35US AnaeroGen™ (for 3.5L jar) 10 sachets/box
Cat. no. CN025A CampyGen™ (for 2.5L jar) 10 sachets/box
Cat. no. CN035A CampyGen™ (for 3.5L jar) 10 sachets/box

INTENDED USE

Oxoid CampyGen™ and AnaeroGen™ are self-contained gas generating systems that are used in closed environmental chambers to rapidly generate microaerophilic and anaerobic atmospheres, respectively. CampyGen™ is recommended for use in producing microaerophilic conditions which are essential for the isolation and growth of Campylobacter while AnaeroGen™ is recommended for establishing an oxygen depleted environment essential for obligate anaerobic microorganisms.

SUMMARY

Nutrients, moisture, pH, temperature, and atmosphere are all factors which are important in the growth of microorganisms. CampyGen™ and AnaeroGen™ are gas generating systems that establish the necessary environments for organisms that require reduced and depleted oxygen concentration. The generators produce microaerophilic and anaerobic atmospheres without the need for catalyst or the addition of water. Each generator is activated immediately upon removal from the mylar pouch.

When placed in a sealed jar, both gas generating sachets produce carbon dioxide simultaneous to rapidly absorbing the atmospheric oxygen in the jar. The AnaeroGen™ sachet will reduce the oxygen level in the jar to below 1% within 30 minutes and results in 9-13% carbon dioxide. The CampyGen™ sachet produces 5% O 2 , 10% CO 2 , and 85% N 2 . Unlike other gas generating systems, CampyGen™ and AnaeroGen™ do not produce hydrogen gas, thereby eliminating the chance of explosion.

The active component within each gas generating paper cachet is ascorbic acid. Although the reaction of ascorbic acid with oxygen is exothermic, the temperature of the paper sachet will not exceed 65ºC.

FORMULA

Ascorbic acid is the reactive component within each paper sachet.

STORAGE AND SHELF LIFE

Storage: Upon receipt store at 2-25ºC. Products should not be used if there are any signs of deterioration (tears), or if the expiration date has passed. Protect from excessive heat and freezing.

PRECAUTIONS

PROCEDURE

Specimen Collection: Consult listed references for information on proper specimen collection. (2-5)

Method of Use:

1. Place the inoculated media plates in the appropriate jar. It is recommended that disposable plastic petri dishes of the vented variety be used in order to aid in the transfer of gas between the interior and exterior of the plates.

2. Tear open the foil pouch at the indicated tear-nick and remove the paper sachet from within.

3. Immediately place the paper sachet in the plate carrier clip within the jar. It is recommended that an Anaerobic Indicator Strip (Cat. no. BR55) be used with the AnaeroGen™ gas generator. The indicator strip should be placed in the jar and used as a visual check that the anaerobic conditions have been achieved and maintained. The indicator strip will turn pink upon exposure to air and will become colorless once oxygen is depleted in the sealed jar.
Note: Indicator Strip must be placed in the jar and the lid immediately attached without delay, in order for the strips to perform properly.

Note: Upon exposure to air, the paper sachet will become warm to the touch.

4. Seal the jar lid immediately.

Note: Do not exceed one minute from the time taken between opening the foil sachet and sealing the jar. Extended exposure to air results in loss of reactivity and microaerophilic/anaerobic conditions may not be achieved in the jar.

5. Remove the plates after the appropriate incubation period and examine for growth and typical colonial morphology. If further incubation is required then a fresh sachet must be used following steps 2-5 above.

6. Discard exhausted sachets with the appropriate laboratory waste.

Note: Upon removal of the AnaeroGen™ after incubation, the paper sachet will retain a small amount of reactivity and will warm up. The sachets should therefore be allowed to cool to room temperature before disposing.

INTERPRETATION OF RESULTS

Consult listed references for the identification of colony morphology and further biochemical tests required for identification. (2-5)

LIMITATIONS

It is essential that the gas generating paper sachet be placed and sealed in the jar within one minute of its removal from the outer foil sachet. Extended exposure to air will result in loss of reactivity and full microaerophilic/anaerobic conditions may not be achieved.

MATERIALS REQUIRED BUT NOT PROVIDED

Standard microbiological supplies and equipment such as loops, other culture media, slides, staining supplies, microscopes, incinerators, incubation jars, and incubators, etc., as well as serological and biochemical reagents, are not provided.

QUALITY CONTROL

Test Organisms Reaction
CampyGen™:
Campylobacter jejuni
ATCC ® 33291
Growth
AnaeroGen™:
Clostridium perfringens
ATCC ® 13124
Growth

PHYSICAL APPEARANCE

CampyGen™ and AnaeroGen™ should appear as white paper sachets individually packaged within a foil pouch.

CampyGen and AnaeroGen

CampyGen™ and AnaeroGen™ Gas Generating Systems.

REFERENCES

1. Anderson, N.L., et al. Cumitech 3A; Quality Control and Quality Assurance Practices in Clinical Microbiology , Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.

2. Jorgensen., et al., Manual of Clinical Microbiology , American Society for Microbiology, Washington D.C.

3. Tille, P., et al. Bailey and Scott's Diagnostic Microbiology , C.V. Mosby Company, St. Louis, MO.

4. Isenberg, H.D. Clinical Microbiology Procedures Handbook , Vol. I & II. American Society for Microbiology, Washington, D.C.

5. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology , J.B. Lippincott Company, Philadelphia, PA.

6. Van Horn, K.G., et al. 1997. Journal of Clinical Microbiology ; Vol. 35, No. 8, p. 2170-2173. American Society for Microbiology.

This document is provided for general product information only. It does not replace the manufacturer's product insert. Always refer to the actual product insert for procedural use and for most recent product information.


ATCC is a registered trademark of the American Type Culture Collection.
AnaeroGen and CampyGen are trademarks of Oxoid Ltd., Basingstoke, Hampshire, England.

IFU-10317[A]