Rapid test for the detection of Colistin resistance via expression of mcr-1 gene on bacterial colony from culture. Not for in vitro diagnostic use
The detection limit was determined using purified recombinant enzymes MCR detection threshold: 300pg/mL.
Validation on a reference strain bank
NG-Test MCR-1 was evaluated on 44 clinical strains at NCR Kremlin bicêtre-Paris (AMR French Reference Center), 117 clinical strains at ANSES-Lyon (National Health Security Agency) and 123 clinical strains at CNR-Clermont Ferrand. (Reference National Center).
A total of 284 Strains were evaluated.
Sensitivity: 100% Confidence interval 95% : 96.3% to 100%
Specificity: 98.3% Confidence interval 95% : 95.3% to 99.4%
Considering targeted MCR-1 enzyme, all results were correlated to the genotype of strains determined by PCR analysis.
NG-Test MCR-1 detects some MCR-2 variants.