Leeds Acinetobacter Medium is a selective and differential medium intended for use as a plating technique to obtain a pure culture of Acinetobacter.
The selective components in Leeds Acinetobacter Medium are traditionally cefsulodin and cephradine, which inhibit the growth of non-Acinetobacter gram-negative bacteria, and vancomycin, which inhibits the growth of gram-positive bacteria. Acinetobacter Medium substitutes antibiotics for the some of the antibiotics in the original reference. The presence of fructose and sucrose and neutral red, a pH indicator, allows the differentiation of bacteria based on the products released when the bacteria use fructose and sucrose as a nutrient source. The colonies of those that release acidic products are yellow, while the colonies of those that release alkaline products are red.
Acinetobacter spp. will produce light pink mucoid colonies with a pink to mauve color diffused into the medium. The colonies are circular, convex, smooth, and opaque with entire margins of 1 to 2 mm in diameter after 24 hours at 35 degrees C.