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HardyCHROM™ O157, 15x100mm plate, order by the package of 10, by Hardy Diagnostics

A selective and differential medium recommended for the isolation of enterohemorrhagic E.coli O157 from food, veterinary, and environmental sources. Chromogenic substances in the media facilitate detection by colony color. Not for human diagnostic use.


Escherichia coli O157 is a pathogen responsible for outbreaks of serious food-borne disease. It has been established that cattle are the major reservoir for E. coli O157, with outbreaks of disease directly associated with the consumption of bovine food products. Traditional selective agars have been based on the ability of the organism to ferment either sorbitol or rhamnose, and the absence of beta-glucuronidase activity in order to isolate E. coli O157.

HardyCHROM™ O157 was developed as a media which is capable of differentiating E. coli O157 from non-E. coli O157 on the basis of colony color due to the presence of specific chromogenic substrates. These chromogens are specific biochemical compounds that produce a visible and qualitative color change when degraded by specific microbial enzymes. E. coli O157 produce purple-pink colored colonies on the plate. Organisms other than E. coli O157 will be inhibited, or appear as blue colonies.

HardyCHROM™ O157 provides an initial screen intended to isolate colonies for further testing. It is necessary to confirm isolated purple-pink colonies taken from HardyCHROM™ O157 with a latex agglutination test (Cat. no. PL070HD), antisera (Cat. no. 295798), or other test methods for a complete identification. Testing for the H7 antigen (Cat. no. PL079) or verotoxin testing may also be required.

  • See the Instructions For Use (IFU) below (under Technical Information) for the complete procedure with color photos.

  • Hardy Diagnostics was the first company to introduce chromogenic prepared media to the United States in 1996. You can expect Hardy Diagnostics to be the leading innovator in chromogenic detection methods.

  • We offer a handy bench-top flip chart to help you interpret the colors of all our chromogenic media. Please call us to receive your copy! (800) 266-2222.

  • We believe in good patient care, just like you do. Look to Hardy and experience "a culture of service."

    This media is to be stored and incubated in the dark. If you have a light in your refrigerator, incubator, or you leave the media exposed to light on the bench top, Hardy Diagnostics offers free Blok-Boxes. Blok-Box is a reusable cardboard box that holds one sleeve of ten petri plates.

    Please ask Customer Service if you would like a free Blok-Box sent with your order. Customer Service – (800) 266-2222

  • To order this product, enter the quantity you wish to order in the QTY field and click the shopping cart icon. When finished, click My Shopping Cart to proceed to checkout or Continue Shopping to shop for more products.

    Cat No. Description Price Curr UOM Qty  
    G305 HardyCHROM O157
    HDx, 15x100mm plt 10/pk R
    $47.70 USD PK10

    Manufacturer Category Organism
    Hardy Diagnostics: prepared media

    Media, Prepared

    E. coli O157

    Instructions for Use Video SDS


    Brochures and Studies BioSafety Level


    Related Products
    PL070HD E. coliPRO™ O157 Latex Kit, rapid latex agglutination test for E. coli O157, 50 tests, by Hardy Diagnostics